This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/policies/article-withdrawal). This article is being retracted at the request of the Editors. An Expression of Concern was previously published for this article while an investigation was conducted. This retraction notice replaces the earlier Expression of Concern. In response to several reports concerning the above paper, regarding the collection of data, the accuracy of analysis and data presentation, and conclusions presented in the article, RBMO Editors investigated to verify the validity of the issues raised and determine the appropriate status of the paper. The investigation followed Elsevier's policy on publishing ethics, the flowcharts and guidelines provided by COPE, and the ‘REAPPRAISED’ checklist for the evaluation of publication integrity. The corresponding author and their institution have responded ​to the editor's inquiries and are unable to provide the trial data required to resolve the concerns. The authors advised that at the time the research was conducted, local policy required records to be destroyed after 5 years, thus, as the trial data are no longer available, post-publication concerns cannot be further investigated. Note at the time of submission, retention of raw data and data sharing was not a requirement. Additionally, the Editors were made aware that this article had been previously published in the Egyptian Journal of Fertility and Sterility: Khaled R. Zalata, A. A. E. (2013). ‘Combined coenzyme Q10 as an adjuvant for ovulation induction in polycystic ovary syndrome’, The Egyptian Journal of Fertility and Sterility, 17(2), pp. 12-15. doi: 10.21608/egyfs.2013.257474 The previous publication was not referred to in this article. One of the conditions of submission of a paper for publication is that authors declare explicitly that their work is original and has not appeared in a publication elsewhere. Re-use of any data should be appropriately cited. Without the possibility of publishing a correction or explanation, the data presented in the paper remain unreliable and already form part of the published record in the previous article, and therefore, in line with COPE recommendations, the journal Editors agree that retraction is appropriate. RETRACTED: Combined coenzyme Q10 and clomiphene citrate for ovulation induction in clomiphene-citrate-resistant polycystic ovary syndromeReproductive BioMedicine OnlineVol. 29Issue 1PreviewThis article has been retracted: please see Elsevier Policy on Article Withdrawal ( https://www.elsevier.com/about/policies/article-withdrawal ). Full-Text PDF

Introduction/Background Systematic paraaortic and pelvic lymphadenectomy is the standard of surgical staging in suspected early epithelial ovarian cancer, However, this procedure is associated with high morbidity. The concept of sentinel lymph node is to determine whether the cancer has spread to the first lymph node, If the sentinel node is negative for malignancy, then there is a high likelihood that the cancer has not spread to other lymph nodes, so if we can prove its accuracy, we can skip complete lymphadenectomy and its co morbidities. Objectives To assess the feasibility and the accuracy of the sentinel node procedure in early ovarian cancer. Methodology this is a prospective single-arm study included patients with presumed early stages epithelial ovarian cancer planned for staging laparotomy. After abdominal exploration and before removal of the ovary, 0.5 ml of methylene blue dye was injected on the dorsal and ventral side of the ovarian ligament and the infundiblo-pelvic ligament. The pelvic and lumboaortic retroperitoneum is then accessed and inspected to identify and remove the sentinel nodes, then staging is completed as usual including systematic pelvic and paraaortic lymphadenectomy. Results 37 patients were included. Sentinel nodes were identified in 20 patients (detection rate, 54%). 3 patients had positive nodes out of 4 patients with lymphatic dissemination, (sensitivity, 75%) false-negative rate, 5%; negative predictive value, 93.7%, 3 (8%) intra- and 1 postoperative complication occurred. Conclusion The detection of sentinel node in early epithelial ovarian cancer is low using blue dye alone. However, the sentinel node procedure is feasible and has the potential to provide reliable and useful information on nodal status and may allow the avoidance of systematic lymphadenectomy in the future. Disclosures Nothing to disclose

•Decreased visfatin gene expression and some antioxidant have an important pathophysiological factor of preeclampsia leading to complication in pregnancy. So, we recommend that all pregnant women should be supplemented with antioxidants to prevent overwhelming effect of oxidative stress.

Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder and the criteria are specified by common complex genetic hyperandrogenism, oligomenorrhea or amenorrhea and polycystic ovary morphology.It is a leading cause of female infertility.The prevelance of PCOS among reproductive age women has been estimated to be 4-12%.The association between PCOS and FSH receptor (FSHR) polymorphism attracts wide attention.The aim of the present study was to evaluate whether polymorphism of FSHR at Ala307Thr codon is associated with PCOS and with

Susceptibility to preeclampsia is believed to have a genetic component. Several studies have reported associations between polymorphisms of oxidative stress- related genes and preeclampsia. The aim of the present study was to study the polymorphisms in anti-oxidant genes glutathione S-transferase P1 (GSTP1) and Mn- superoxide dismutase (Mn-SOD) in patients with of preeclampsia. Seventy four preeclampsia patients and fifty age-matched healthy pregnant female controls were genotyped for GSTP1 and Mn-SOD. DNA was extracted from peripheral blood of all patients and control women. DNA analysis was carried out by polymerase chain reaction (PCR), and then digestion of the PCR products by restriction enzymes (RFLP) for both genes was performed. As regards GSTP1, the carriers of Val (G) allele were significantly more frequent among preeclampsia patients when compared to the control group (45.95% Vs. 19%, X 2 =10.40, OR=2.418, 95%CI (1.3698 - 4.269); p=0.0023). Preeclampsia patients had a lower frequency of GSTP1 Ile/Ile (AA) genotype (31.1% Vs. 68% in control; X 2 =13.09, OR=0.457, 95%CI (0.241 - 0.866); p =0.0164). The frequency of GSTP1 Ile/Val (AG) and Val/Val (GG) genotypes was higher in preeclampsia patients than control (68.9%Vs. 32%; X 2 = 12.83, OR=2.154, 95%CI (1.106 - 4.194); p =0.0241). However, non significant frequencies differences as regards Mn-SOD genotypes or alleles were found between preeclampsia patients and control. It could be concluded that, pregnant Egyptian women carrying the Val (G) allele of GSTP1 GSTP1 -105 Ile →Val (-313 A to G) polymorphism may be more susceptible to preeclampsia either in homozygous or heterozygous state.

Objective: To assess the relationship between paternal age and the outcome of percutaneous epididymal sperm aspiration-intracytoplasmic sperm injection (ICSI) in patients with congenital bilateral absence of the vas deferens (CBAVD).Design: Prospective cohort study.Setting: In vitro fertilization centers.Patient(s): Eighty-five (male) CBAVD patients who underwent percutaneous epididymal sperm aspiration with ICSI were divided into 3 age groups; <30 years (n = 27); aged 30-40 years (n = 32); and aged >40 years (n = 26).Intervention(s): History taking, clinical examination, semen analysis, percutaneous epididymal sperm aspiration with subsequent ICSI.Main Outcome Measure(s): Sperm retrieval, clinical pregnancy, and "take-home baby.''Result(s): The number of sperm retrieved by percutaneous epididymal sperm aspiration significantly decreased with advancing age in the studied groups (0.63, 0.31, and 0.18 million, respectively), concomitant with significant decreases in the outcomes of clinical pregnancy (55.5%, 43.7%, and 23.1%, respectively) and "take-home baby'' (48.1%, 40.6%, and 11.5%, respectively). Male age was significantly negatively correlated with retrieved sperm count, sperm motility, sperm vitality, and normal sperm morphology. Multiple logistic regression, adjusted for confounding factors, with male age, and sperm count obtained using percutaneous epididymal sperm aspiration, was significant. The receiver operating characteristic curve showed that a sperm count of 0.55 million as a cutoff value could predict clinical pregnancy with an accuracy of 77.4%, with 82.9% sensitivity and 62% specificity, and "take-home baby'' with an accuracy of 81.7%, with 93.1% sensitivity and 62.5% specificity.Conclusion(s): In cases of CBAVD, male age has a negative effect on retrieved-sperm count, motility, vitality, and normal sperm morphology; number of retrieved sperm predicted both clinical pregnancy and "take-home baby'' outcomes in subsequent ICSI. (C) 2015 by American Society for Reproductive Medicine.

PURPOSE:Polycystic ovary syndrome (PCOS) is associated with oxidative stress (OS) and serum superoxide dismutase (SOD) activity has been reported with mixed results. The objective of this study was to examine the activity of SOD both in the serum and FF from women with PCOS undergoing ICSI, as well as the expression of Cu/Zn-SOD mRNA in the cells recovered from the FF. METHODS:Forty women undergoing an ICSI trial were divided into: group I, included 20 PCOS cases, group II included 20 age-matched controls with tubal factor infertility. Both groups were similarly stimulated. A total of 204 metaphase II (MII) oocytes were aspirated; (108) from PCOS, and (96) from the control group. SOD activities in the serum and FF, as well as Cu/Zn-SOD (SOD1) mRNAs in follicular fluid (FF) cells were analyzed. RESULTS:There was a statistically highly significant decrease (p < 0.001) both in the mean serum SOD (45.56 ± 18.06) and FF SOD activity (42.49 ± 11.46) in PCOS than the control group (77.38 ± 7.82), (74.37 ± 6.15) respectively. The mean relative levels of Cu, Zn SOD mRNAs was significantly lower (p < 0.001) in cells isolated from the FF in PCOS (0.36 ± 0.14) than the control group (0.81 ± 0.15). SOD activity in FF had no effects on fertilization rate (p > 0.05), or embryo quality after intracytoplasmic sperm injection (ICSI). CONCLUSION:Although decreased SOD activity in FF has no effect on fertilization rate and/or embryo quality, serum SOD activity could be a clinical parameter for determining systemic oxidative stress in PCOS.

PURPOSE:To study the effect of combined oral prednisolone and LMW heparin in ICSI in women with previously unexplained, failed implantation.METHODS:A prospective quasi-randomized, controlled trial was conducted at a university teaching hospital and a private practice setting. A total of 334 cycles (women with previously unexplained, failed one or two ICSI attempts) were assigned randomly to receive standard treatment or combined prednisolone (20 mg/day), starting on the first day of ovarian stimulation and LMW heparin 1 mg/kg/day starting 1 day after oocyte retrieval in addition to standard treatment.RESULTS:The mean age, number of previously failed IVF attempts, and basal FSH levels were comparable between both groups. The pregnancy and the implantation rates were significantly different between the study and control groups.CONCLUSIONS:A combination of oral prednisolone and low molecular weight heparin may have a significant effect on pregnancy and implantation rates in prior unexplained, failed implantation.

This prospective randomized controlled trial evaluated the effect of combined oral coenzyme Q10 (CoQ10) and clomiphene citrate for ovulation induction in clomiphene-citrate-resistant polycystic ovary syndrome (PCOS). A total of 101 infertile women with PCOS resistant to clomiphene citrate were randomized either to combined CoQ10 and clomiphene citrate (51 patients, 82 cycles) or to clomiphene citrate alone (50 patients, 71 cycles). The outcome measures were number of follicles, serum oestradiol, serum progesterone, endometrial thickness and ovulation, clinical pregnancy and miscarriage rates. Numbers of follicles >14 mm and ≥18 mm were significantly higher in the CoQ10 group. Endometrial thickness on the day of human chorionic gonadotrophin was significantly greater in the CoQ10 group (8.82 ± 0.27 mm versus 7.03 ± 0.74 mm). Ovulation occurred in 54/82 cycles (65.9%) in the CoQ10 group and 11/71 cycles (15.5%) in the control group. Clinical pregnancy rate was significantly higher in the CoQ10 group (19/51, 37.3%) versus the control group (3/50, 6.0%). Combination of CoQ10 and clomiphene citrate in the treatment of clomiphene-citrate-resistant PCOS patients improves ovulation and clinical pregnancy rates. It is an effective and safe option and can be considered before gonadotrophin therapy or laparoscopic ovarian drilling.

OBJECTIVES:To study the agreement of preoperative curettage and definitive histology after hysterectomy as well as the correlation of time factor and hormonal therapy to this agreement.METHODS:This retrospective study was done in the Departments of Obstetrics and Gynecology and Pathology, Faculty of Medicine, Mansoura University, Egypt. Pathology reports of patients exposed to dilatation and curettage followed by hysterectomy during the period from May, 2004 to April, 2011 were reviewed.RESULTS:We reviewed 83 cases who fulfilled the inclusion criteria. There were non-significant differences between findings of D&C and hysterectomy specimens (P = 0.42). The concordance rate between D&C and hysterectomy was 79.5%. One case of endometrial carcinoma (1.2%), one case of simple endometrial hyperplasia, one case of atrophic endometrium, and two cases of endometrial polyp were diagnosed only after hysterectomy (4.8%). Furthermore, histologic examination of hysterectomy specimens confirmed only 8 of 13 cases (61.5%) of endometrial hyperplasia with atypia. No significant correlation of time factor and hormonal therapy to the agreement between findings of D&C and hysterectomy specimens (P = 0.58 & 0.19 respectively).CONCLUSION:There was no significant difference between preoperative endometrial histology and hysterectomy specimens (P = 0.41).This agreement had not been significantly affected by time interval and hormonal therapy. Future researches with larger number of cases are needed to confirm or disagree with our findings.