Complementary DNA encoding the Na/Ca,K-exchanger was isolated from bovine retina cDNA libraries. The complete full-length cDNA is approximately 4 kb long and contains an open reading frame of 3597 bp. The deduced amino acid sequence corresponds to a protein of 1199 amino acids with a calculated molecular weight of approximately 130 kDa. Hydrophobicity analysis revealed the presence of two alternating sets of hydrophobic and hydrophilic domains. There also exists a hydrophobic region at the N-terminus which may be part of a cleavable signal peptide. The protein shares limited sequence homology with the Na/Ca-exchanger from cardiac sarcolemma. Northern blot analysis indicates that the approximately 6 kb transcript is highly specific for retinal tissue. Insect cells infected with recombinant baculovirus bearing the full-length cDNA express a functional Na/Ca,K-exchanger with an apparent relative molecular weight of approximately 210 kDa, as determined by Western blotting.

Annals of the New York Academy of SciencesVolume 639, Issue 1 p. 234-244 Biochemical and Molecular Characterization of the Sodium-Calcium Exchanger from Bovine Rod Photoreceptorsa ANITA ACHILLES, Corresponding Author ANITA ACHILLES Max-Planck-Institut für Biophysik Abteilung für Molekulare Membranbiologie D-6000 Frankfurt am Main 71, Federal Republic of GermanybAddress for correspondence: Max-Planck-Institut für Biophysik, Abteilung für Molekulare Membranbiologie, Heinrich-Hoffmann Str. 7, D-6000 Frankfurt am Main 71, Federal Republic of Germany.Search for more papers by this authorUTE FRIEDEL, UTE FRIEDEL Max-Planck-Institut für Biophysik Abteilung für Molekulare Membranbiologie D-6000 Frankfurt am Main 71, Federal Republic of GermanySearch for more papers by this authorWINFRIED HAASE, WINFRIED HAASE Max-Planck-Institut für Biophysik Abteilung für Molekulare Membranbiologie D-6000 Frankfurt am Main 71, Federal Republic of GermanySearch for more papers by this authorHELMUT REILÄNDER, HELMUT REILÄNDER Max-Planck-Institut für Biophysik Abteilung für Molekulare Membranbiologie D-6000 Frankfurt am Main 71, Federal Republic of GermanySearch for more papers by this authorNEIL J. COOK, NEIL J. COOK Max-Planck-Institut für Biophysik Abteilung für Molekulare Membranbiologie D-6000 Frankfurt am Main 71, Federal Republic of GermanySearch for more papers by this author ANITA ACHILLES, Corresponding Author ANITA ACHILLES Max-Planck-Institut für Biophysik Abteilung für Molekulare Membranbiologie D-6000 Frankfurt am Main 71, Federal Republic of GermanybAddress for correspondence: Max-Planck-Institut für Biophysik, Abteilung für Molekulare Membranbiologie, Heinrich-Hoffmann Str. 7, D-6000 Frankfurt am Main 71, Federal Republic of Germany.Search for more papers by this authorUTE FRIEDEL, UTE FRIEDEL Max-Planck-Institut für Biophysik Abteilung für Molekulare Membranbiologie D-6000 Frankfurt am Main 71, Federal Republic of GermanySearch for more papers by this authorWINFRIED HAASE, WINFRIED HAASE Max-Planck-Institut für Biophysik Abteilung für Molekulare Membranbiologie D-6000 Frankfurt am Main 71, Federal Republic of GermanySearch for more papers by this authorHELMUT REILÄNDER, HELMUT REILÄNDER Max-Planck-Institut für Biophysik Abteilung für Molekulare Membranbiologie D-6000 Frankfurt am Main 71, Federal Republic of GermanySearch for more papers by this authorNEIL J. COOK, NEIL J. COOK Max-Planck-Institut für Biophysik Abteilung für Molekulare Membranbiologie D-6000 Frankfurt am Main 71, Federal Republic of GermanySearch for more papers by this author First published: December 1991 https://doi.org/10.1111/j.1749-6632.1991.tb17310.xCitations: 6 a This work was supported by the Deutsche Forschungsgemeinschaft (SFB 169 Projekt C4) and a Leibniz-Programm Grant to Prof. Hartmut Michel. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume639, Issue1Sodium-Calcium Exchange: Proceedings of The Second International ConferenceDecember 1991Pages 234-244 RelatedInformation