Data from a controlled environment experiment investigating effects of temperature on maturation of Leptosphaeria maculans pseudothecia were used to derive equations describing the times until 30% or 50% of pseudothecia were mature as a function of temperature. A wetness sensor was developed to estimate the oilseed rape debris wetness and operated with debris exposed in natural conditions in 2000 and 2001. The maturation of L. maculans pseudothecia on debris and concentrations of airborne L. maculans ascospores were observed from 1999 to 2004. There were considerable differences between years, with the first mature pseudothecia observed in September in most years. There were linear relationships between the first date when 10% of maximum ascospore release was observed and the dates when 30% or 50% of pseudothecia were mature. By summing the daily temperature-dependent rate of pseudothecial maturation for days after I August with rainfall > 0.5 mm, the dates when 30% or 50% of pseudothecia were mature were predicted. There was good agreement between predicted and observed dates when 30% or 50% of pseudothecia were mature. These equations for predicting the timing of L. maculans ascospore release could be incorporated into schemes for forecasting, in autumn, the severity of phoma stem canker epidermics in the following spring/summer in the UK.

Methods to assess light leaf spot (Pyrenopeziza brassicae) on winter oilseed rape cultivars were compared in laboratory, controlled‐environment and field experiments. In controlled‐environment experiments with seedling leaves inoculated at GS 1,4, the greatest differences in percentage area affected byP. brassicaesporulation were observed with inoculum concentrations of 4 × 103or 4 × 104spores mL−1, rather than 4 × 102or 4 × 105spores mL−1, but older leaves had begun to senesce before assessment, particularly where they were severely affected byP. brassicae. In winter oilseed rape field experiments, a severe light leaf spot epidemic developed in 2002/03 (inoculated, September/October rainfall 127·2 mm) but not in 2003/04 (uninoculated, September/October rainfall 40·7 mm). In‐plot assessments discriminated between cultivars best in February/March in 2003 and June in 2004, but sometimes failed to detect plots with many infected plants (e.g. March/April 2004). Ranking of cultivar resistance differed between seedling experiments done under controlled‐environment conditions and field experiments. The sensitivity of detection ofP. brassicaeDNA extracted from culture was greater using the PCR primer pair PbITSF/PbITSR than using primers Pb1/Pb2.P. brassicaewas detected by PCR (PbITS primers) in leaves from controlled‐environment experiments immediately and up to 14 days after inoculation, and in leaves sampled from field experiments 2 months before detection by visual assessment.

The Representative Soil Sampling Scheme of England and Wales has recorded information on the soil of agricultural land in England and Wales since 1969. It is a valuable source of information about the soil in the context of monitoring for sustainable agricultural development. Changes in soil nutrient status and pH were examined over the period 1971-2001. Several methods of statistical analysis were applied to data from the surveys during this period. The main focus here is on the data for 1971, 1981, 1991 and 2001. The results of examining change over time in general show that levels of potassium in the soil have increased, those of magnesium have remained fairly constant, those of phosphorus have declined and pH has changed little. Future sampling needs have been assessed in the context of monitoring, to determine the mean at a given level of confidence and tolerable error and to detect change in the mean over time at these same levels over periods of 5 and 10 years. The results of a non-hierarchical multivariate classification suggest that England and Wales could be stratified to optimize future sampling and analysis. To monitor soil quality and health more generally than for agriculture, more of the country should be sampled and a wider range of properties recorded.

Despite differences in climate and in timing of light leaf spot epidemics between Poland and the UK, experiments provided no evidence that there are epidemiological differences between populations of Pyrenopeziza brassicae in the two countries. Ascospores of Polish or UK P. brassicae isolates germinated on water agar at temperatures from 8 to 24 ° C. After 12 h of incubation, percentages of ascospores that germinated were greatest at 16 ° C: 85% (Polish isolates) and 86% (UK isolates). The percentage germination reached 100% after 80 h of incubation at all temperatures tested. The rate of increase in germ tube length increased with increasing temperature from 8 to 20 ° C but decreased from 20 to 24 ° C, for both Polish and UK isolates. Percentage germination and germ tube lengths of UK P. brassicae ascospores were less affected by temperature than those of conidia. P. brassicae produced conidia on oilseed rape leaves inoculated with ascospores or conidia of Polish or UK isolates at 16 ° C with leaf wetness durations from 6 to 72 h, with most sporulation after 48 or 72 h wetness. Detection of both mating types of P. brassicae and production of mature apothecia on leaves inoculated with mixed Polish populations suggest that sexual reproduction does occur in Poland, as in the UK.

In controlled environment experiments, sporulation ofPyrenopeziza brassicaewas observed on leaves of oilseed rape inoculated with ascospores or conidia at temperatures from 8 to 20°C at all leaf wetness durations from 6 to 72 h, except after 6 h leaf wetness duration at 8°C. The shortest times from inoculation to first observed sporulation (l0), for both ascospore and conidial inoculum, were 11–12 days at 16°C after 48 h wetness duration. For both ascospore and conidial inoculum (48 h wetness duration), the number of conidia produced per cm2leaf area with sporulation was seven to eight times less at 20°C than at 8, 12 or 16°C. Values of Gompertz parametersc(maximum percentage leaf area with sporulation),r(maximum rate of increase in percentage leaf area with sporulation) andl37(days from inoculation to 37% of maximum sporulation), estimated by fitting the equation to the observed data, were linearly related to values predicted by inserting temperature and wetness duration treatment values into existing equations. The observed data were fitted better by logistic equations than by Gompertz equations (which overestimated at low temperatures). For both ascospore and conidial inoculum, the latent period derived from the logistic equation (days from inoculation to 50% of maximum sporulation,l50) ofP. brassicaewas generally shortest at 16°C, and increased as temperature increased to 20°C or decreased to 8°C. Minimum numbers of spores needed to produce sporulation on leaves were ≈25 ascospores per leaf and ≈700 conidia per leaf, at 16°C after 48 h leaf wetness duration.

Relationships between the incidence and severity of brown foot rot and of pathogenic fungi, determined by diagnostic and quantitative PCR, were investigated during the growth of nine winter wheat crops in three cropping seasons. Microdochium nivale vars nivale and majus were the only brown foot rot pathogens present in significant amounts. Relationships between disease symptoms and amounts of pathogen DNA were often weak in early spring (when shoot-base symptoms are usually most difficult to ascribe to particular pathogens by visual examination) because of indistinct symptoms and small amounts of pathogen. Relationships were strongest during stem elongation. The amount of M. nivale in the tissues tended to decline in the summer as the plants matured, apparently disappearing partially from necrotic lesions to which it contributed, resulting in a weakened relationship between symptoms and pathogen DNA. Regression analyses of brown foot rot on amounts of M. nivale DNA for different wheat cultivars generally produced lines with similar slopes but were often most significant for the cultivar with most eyespot resistance (i.e. with least confounding eyespot) or most apparently genuine brown foot rot. DNA of Fusarium spp. was rarely present in amounts sufficient to quantify.

The effects of supplying the fertilizer nitrogen (N) as a recommended quantity of ammonium nitrate or as a commonly used dose of poultry manure on yield of sugarbeet infected with Beet mild yellowing virus (BMYV) or Beet yellows virus (BYV) were studied in field experiments at IACR-Broom's Barn in 1990, 1991 and 1992. Three N fertilizer treatments comprising Zero (N0), standard rate of 110 kg N/ha (N1) and poultry manure equivalent to c. 300 kg/ha of available N (N2) were applied to plots which were uninoculated or were subsequently inoculated with either BMYV or BYV. Averaged over virus treatments, N1 increased sugar yields by 23% relative to N0: there was no further increase when N2 was applied. When averaged over N treatments, early virus yellows infection reduced the sugar yields by 23%. Generally there was no significant interaction between N supply and virus infection. There was no evidence that the large N supply could reduce the yield effect of virus yellows infection, as had previously been thought. Crops infected from late July produced similar yields to uninoculated controls. The main effect of virus yellows was to reduce the efficiency of radiation conversion even when account was taken of the light intercepted by yellow foliage. Whilst the N2 treatment helped to maintain a green leaf cover throughout the season on virus yellows infected crops, it had no effect on virus replication. Beet processing quality was impaired by increasing the N supply and by virus infection, but again there were generally no significant interactions between infection and N rate.

Results from field experiments with mobile pests and air-borne pathogens are subject to bias as a result of inter-plot interference. Serially balanced designs (SBDs) allow interference to be estimated but other designs may be better for decreasing such effects. To investigate this, systematic replicated designs, comparing sprays applied at different times to control powdery mildew of spring barley, were sited in 2 years alongside SBDs testing similar treatments. Yields of grain and assessments of mildew on the leaves were analysed. Results from the balanced designs provided strong evidence of interference in both years but not in a third (when the systematic design was omitted). Estimates of treatment effects from the systematic designs were often, but not consistently, greater than corresponding estimates from the SBDs. A method of analysis from Draper & Guttman (1980) was adapted to produce estimates of the differences between treatments as if applied to all plots of an experiment; this showed larger differences between treatments than the conventional analysis in 1975 and 1976 (when there was appreciable interference), but failed in 1977 when interference was slight. This method fails when applied to the systematic designs; SBDs (which are a subset of all designs in randomized blocks) are probably optimal for this type of analysis. The difficulties of analysing data in the form of percentages or proportions (with consequent non-orthogonality) are discussed.

SummaryAscospores of both A‐group and B‐group Leptosphaeria maculans germinated at temperatures from 5–20°C on distilled water agar or detached oilseed rape leaves. After 2 h of incubation on water agar, some A‐group ascospores had germinated at 10–20°C and some B‐group ascospores had germinated at 5–20°C. The percentages of both A‐group and B‐group ascospores that had germinated after 24 h of incubation increased with increasing temperature from 5–20°C. The observed time (Vo50) which elapsed from inoculation until 50% of the spores had germinated was shorter for B‐group than for A‐group ascospores. Germ tube length increased with increasing temperature from 5–20°C for both ascospore groups. Germ tubes from B‐group ascospores were longer than germ tubes from A‐group ascospores at all temperatures tested, but the mean diameter of germ tubes from A‐group ascospores (1.8 μm) was greater than that of those from B‐group ascospores (1.2μm) at 15°C and 20°C. The average number of germ tubes produced from A‐group ascospores (3.8) was greater than that from B‐group ascospores (3.1) after 24 h of incubation at 20°C, on both water agar and leaf surfaces. Germ tubes originated predominantly from interstitial cells or terminal cells of A‐group or B‐group ascospores, respectively, on both water agar and leaf surfaces. Hyphae from A‐group ascospores grew tortuously with extensive branching, whilst those from B‐group ascospores were predominantly long and straight with little branching, whether the ascospores were produced from oilseed rape debris or from crosses between single ascospore isolates, and whether ascospores were germinating on water agar or leaf surfaces.

Three experiments on winter wheat, each lasting 5 years and on different soil types, were used to test the effects of incorporating different amounts of straw, mainly to determine the importance of achieving uniform distribution to avoid adverse effects on grain yield. Decreases in crop growth and/or grain yield as a consequence of incorporating straw were detected in the first year. The decreases were much larger in one experiment where straw was imported and applied to soil that had been fallowed for 12 months before sowing the wheat than in the other two where the straw was incorporated following the harvest of a winter wheat crop. In the subsequent 4 years, incorporating up to 20 t straw/ha had no significant effects on grain yield but there were some significant effects on concentrations and uptakes of N, P and K, especially on the heavier textured soils. The effects on crop growth and yield that were detected in the first year on each site are tentatively attributed to decreases in available N representing that which was required to support the decomposition of the incorporated straw. The relative lack of significant effects in subsequent years seems to imply that a significant proportion of this N was remineralized relatively quickly, and thus available to support the decomposition of the straw that was incorporated in the second year and, after further recycling, in the years after that. Eyespot, caused by the fungus Pseudocercosporella herpotrichoides, was decreased by incorporating straw but there were few significant effects on other diseases. The results provide a generally reassuring message for farmers in suggesting that on most, if not all, soils there is little cause for concern about the consequences of incorporating even large amounts of wheat straw before sowing a further crop of winter wheat.

Diagnostic and quantitative polymerase chain reaction (PCR) provided clarification of the causes of symptoms and the extent of infection by eyespot (Tapesia spp.) and sharp eyespot (Rhizoctonia cerealis) on winter wheat at early growth stages. Disease assessments made before stem extension, when decisions to apply fungicides are usually made, often did not agree with the pathogen diagnoses using PCR, suggesting that such early visual diagnoses may be unreliable. Visual and PCR diagnoses made on stems in summer generally supported each other, but there were often discrepancies in relating disease severity to amounts of pathogen present when determined by regression analyses of incidence or severity of symptoms on amount of pathogen DNA. Mixed symptoms caused by different pathogens may sometimes have been confounded. Relationships between symptoms and DNA of eyespot pathogens were less clear on some cultivars, often those with least disease. Sharp eyespot symptoms had a stronger relationship to DNA of its pathogen. Significant regressions often accounted for a small percentage of the variance, suggesting either that pathogens not assayed were contributing to symptoms or that lesions were in some cases persisting longer into the season than pathogen DNA. The frequency of pathogen detection before stem extension was a poor predictor of the amounts of pathogen DNA measured later in the season.

In controlled‐environment experiments, ascospores of both A‐group and B‐groupLeptosphaeria maculanswere able to infect leaves of oilseed rape and produce phoma leaf spot lesions at temperatures from 5 to 20°C and wetness durations from 8 to 72 h after inoculation. Lesions formed on leaves inoculated with B‐group ascospores had few pycnidia and were darker, smaller and less noticable than the larger, pale grey lesions with many pycnidia produced by A‐group ascospores. Lesions formed by A‐group or B‐groupL. maculanson naturally infected winter oilseed rape experimental crops were similar to lesions produced by the two groups on inoculated plants. The greatest numbers of lesions were produced with a leaf wetness duration of 48 h and at temperatures of 15–20°C for both A‐group and B‐group ascospores. As leaf wetness duration and temperature decreased below the optimal values, the number of lesions decreased. The incubation period, estimated as the time from inoculation to the appearance of the first lesions (t1), or the time to the appearance of 50% of the lesions (t50), of B‐group was often shorter than that of A‐groupL. maculans. As temperature decreased below 20°C, the length of the incubation period of both A‐group and B‐groupL. maculansincreased.

In the UK, recommended field trial protocols for assessing within-season effects of insecticides on non-target arthropods in cereals utilise either large (1ha or greater) open plots or small (not less than 10m×10m) enclosed plots. Prior to this study, no direct comparison of the relative effectiveness and reliability in discerning such effects of these two approaches had been attempted. In a 2-year study, the effects of dimethoate and pirimicarb on polyphagous predators were investigated using both small enclosed plots and large open plots in the same experiment. The activity-density of Carabidae, Staphylinidae and Linyphiidae was measured using pitfall traps over at least five pre- and four post-treatment weeks in each year. More species were caught in greater numbers in large open plots than in small enclosed plots. Both approaches caught sufficient individuals to analyse effects of insecticides on whole taxonomic families but too few individuals were caught in small enclosed plots to analyse effects on species other than those most abundant. More individuals were caught per trap and catch variability was less in large open plots than in small enclosed plots. The impact of plot type on pitfall trap catch was greatest for Carabidae and least for Staphylinidae. Treatment with dimethoate led to significant short-term reductions in catches of Carabidae and Linyphiidae, while treatment with pirimicarb had no significant effect on polyphagous predators. Neither of the insecticides applied in 1993 affected pitfall trap catches, in the following year, of predators that were active pre-treatment. No significant interactions were recorded between plot type and insecticide treatment, but low and variable pitfall trap catches in small enclosed plots makes the detection of such interactions difficult. Small enclosed plots, with pitfall traps placed centrally, should not be used in field trials as an alternative to large open plots without modifying sampling methods to increase trap captures and decrease overall variability in numbers caught. The use of more traps, more efficient trapping and greater replication all need to be investigated.

A series of experiments were carried out at 14 sites in the major arable areas of the UK, in the years 1993–94, 1994–95 and 1995–96 to determine the causes and extent of over-winter plant losses of two autumn-sown cultivars of white lupin (Lupinus albus L.). Over the three seasons frost was the major cause of plant losses. Two mechanisms (one known and one not previously reported in the UK) of frost tolerance under field conditions were identified: (i) lignification of the root parenchyma early in the life of the seedling, and (ii) a large vernalization requirement of the main-stem apex, which delays stem elongation in older plants. A model was developed that could be used to predict the susceptibility of these lupin cultivars to the first severe frost of the winter using accumulated thermal time (above a base temperature of 3 °C). The effect of sowing at the beginning or end of a sowing window, calculated to optimize plant architecture the following summer, therefore varied with the weather during the autumn/winter period. A combination of cool autumn weather and late sowing (outside of the sowing window) resulted in plant losses due to a lack of lignification of the root parenchyma. In unusually warm autumn conditions (1994–95 and 1995–96) plants sown at the beginning of the sowing window were well developed before the first frosts occurred, with consequent stem elongation and plant losses. Although losses due to pests were not severe (c. 3 to 5 plants/m2), plants were attacked during the autumn by grey field slugs (Deroceras reticulatum Muller), bean seed flies (Delia platura Meigen), and probably thrips (Thrips angusticeps Uzel, Noctuidae), although this has not been confirmed. The fungal pathogens Fusarium spp. and Botrytis cinerea Pers. caused losses in conjunction with frost damage.

Metarhizium anisopliae has potential as a biological control agent. Included among its hosts are certain insect pests of brassica crops. Brassica species produce isothiocyanates, some of which are known to be fungitoxic. In our study, isothiocyanates inhibited both germination and subsequent growth by M. anisopliae in vitro and its ability to infect insects. Conidia were more sensitive than the mycelium to these compounds, the most fungistatic of which were phenylethyl, 2-chlorophenyl- and allyl-isothiocyanates. Appressorium production in vitro was suppressed by all isothiocyanates except allyl- and propyl-isothiocyanates, which appeared to stimulate appressorium formation. Phenylethyl- and 3-butenyl isothiocyanates, which are present in several of the plant hosts of Phaedon cochleariae, reduced the pathogenicity of M. anisopliae when inoculated insects were exposed to their vapours. These findings have implications for the efficacy of biocontrol of brassica pests by this fungus.

Between 1989 and 1993, 17 experiments tested the effect of cover crop species, sowing date and destruction date on cover crop dry matter (DM) yield, N uptake and on soil mineral nitrogen (SMN) content. All the experiments were carried out in Suffolk, Norfolk, Lincolnshire and Yorkshire on sandy-loam textured soils after crops of cereals or oilseed rape had been harvested. The largest DM yields were obtained with early sowings and averaged 1·6 t/ha. Cover crop N uptake was less dependent upon sowing date and averaged 35 kg N/ha. The average reduction in SMN was from 46 to 32 kg N/ha. Differences between cover crop species were small when compared with season/site variations.Cereal cover crop DM yields were closely related to the thermal time accumulated from the first significant rainfall after sowing, whilst the yields of non-cereal cover crops were more affected by the moisture content of the soil at sowing. The amount of SMN in the soil at sowing had little or no effect on cover crop yield. The yields of cereal cover crops were much more predictable than those of non-cereal cover crops. Water usage by cover crops was estimated to be 20 mm/t DM and large cover crops delayed the onset of leaching and reduced the amount of water leached. However, even in dry autumns and winters, soils are likely to reach field capacity before the following beet crop is sown. Due to their large C[ratio ]N ratio (20[ratio ]1) little N would be mineralized after cover crop destruction. Cover crops comprising volunteer cereals and weeds often performed as well as the other cover crops and in most cases will be the most cost-effective cover crops.

Different management regimes for 1-year rotational set-aside were tested in three experiments that followed winter wheat and started in autumn 1988–90. The regimes included operations that prevented the establishment of volunteers or allowed them to establish and persist until either spring or summer, and also altered the distribution of debris from the winter wheat that preceded the set-aside. For comparison, treatments in the set-aside year also included winter wheat.Samples taken in spring from the first test crop showed that there were few significant or consistent effects on leaf diseases of growing the wheat after different set-aside treatments or after winter wheat. There were significant effects of the set-aside treatments on root and stem base diseases but some of the effects, and the apparent absence of others, are not easily reconciled with current understanding of the biology of the pathogens concerned. In summer, eyespot (Pseudocercosporella herpotrichoides) was most severe after winter wheat and least severe after ryegrass. Severity after the other set-aside treatments did not differ significantly. There was more sharp eyespot (Rhizoctonia cerealis) in plots that had been ploughed at the start of the set-aside year, including those sown with winter wheat, than in those that had not. Brown foot rot (Fusarium spp.) was equally severe where the wheat followed wheat or where it followed set-aside treatments that allowed volunteers to develop, and less so where the development of volunteers was prevented. Take-all (Gaeumannomyces graminis var. tritici) was most severe after winter wheat and more severe after set-aside treatments that allowed volunteers to develop and survive through the winter than after those that did not. Effects of ryegrass (Lolium perenne ssp. multiflorum) on take-all in the following wheat were particularly variable, perhaps because ryegrass is a host of both the take-all fungus and of Phialophora graminicola, one of its principal antagonists.

Between 1989 and 1993, 17 experiments tested the effects of autumn-sown cover crops on the yield, processing quality and N nutrition of subsequent sugarbeet crops. Cover crops had no effect on sugarbeet plant population density or pesticide requirement. In nitrogen response experiments, the mean beet yield at the economic optimum was 83 t/ha. The mean N fertilizer requirement was 96 kg N/ha and the N uptake at maximum yield averaged 180 kg N/ha. Cover crops had no effect on yield, fertilizer requirement or N uptake. In addition, cover crops generally had no effect on the efficiency of N fertilizer use, the mineralization of N from the soil organic matter nor the amount of soil mineral nitrogen at sowing or at harvest of the beet crop. Processing quality was also not affected by cover crops. The cost of growing a cover crop ranged from 0 to 50 £/ha. Since these costs cannot be offset against increases in yields or reduced fertilizer application rates, cover crops need to be low cost, i.e. cheap seed and minimal cultivation. Cover crops using volunteer cereals and weeds or farm-saved grain that are established with a single stubble-cultivation should fulfil these criteria.

The Representative Soil Sampling Scheme of England and Wales was started in 1969. During the 25 year period 1969-1993 mean soil pH values under arable and ley-arable cropping changed little, but under grassland they fell by 0.3 units. Under arable cropping, mean available phosphorus declined whilst available potassium increased fairly steeply for the first eight years then declined again to near the start value by 1993. No significant changes in soil phosphorus and potassium levels were found under ley-arable cropping, but the percentage of fields under ley-arable rotations declined from 33% to 14%. Under grassland, phosphorus and potassium levels both declined, although potassium levels rose initially before falling.For this report England and Wales were also divided into five regions. Wales and the West Country had the low est pH values; these regions together with the Northern region were also found to have declining pH values. Phosphorus and potassium concentrations have been falling in the Northern region which stands out as having the lowest current potassium status with 20% of all fields deficient at index 0.Changes in pH and nutrients are discussed for soils sampled following the main arable crops. The largest changes found were the reductions in phosphorus and potassium in soils after potatoes. In 1993 almost one in ten sugarbeet crop soils had an excessive phosphorus level (index 5+) giving an increased risk of water pollution by this element.Under arable cropping the proportion of soils with low ( < 1.8%) organic matter declined. Average soil organic matter levels for all crops and grass have remained static during the period. It is concluded that agricultural soils in England and Wales have not contributed to an increase in the greenhouse gas carbon dioxide.