1. Mephenesin is oxidatively metabolized in vitro by rat and mouse liver slices to 3-(o-tolyloxy) lactic acid. 2. Mephenesin metabolism was markedly inhibited by increasing substrate concentration, but was not altered by presence of increasing concentrations of the product, 3-(o-tolyloxy)lactic acid, in the medium. 3. Metabolism of mephenesin was readily inhibited by iodoacetic acid, but was unaltered by aldehyde trapping agents at 10-3 M.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSpectrophotometric Determination of 3-o-Tolyloxy-1,2-propanediol (Mephenesin) and Its Metabolite in Plasma and UrineA. R. Maass, P. L. Carey, and A. E. HemingCite this: Anal. Chem. 1959, 31, 8, 1331–1334Publication Date (Print):August 1, 1959Publication History Published online1 May 2002Published inissue 1 August 1959https://pubs.acs.org/doi/10.1021/ac60152a025https://doi.org/10.1021/ac60152a025research-articleACS PublicationsRequest reuse permissionsArticle Views23Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Dougherty (1950, 1951, 1952) reported that cortisone and hydrocortisone had antiphlogistic activity when injected together with a phlogistic agent into the subcutaneous connective tissue of mice. Since as little as 0.08 μg./ml. of steroid dissolved in isotonic saline solution was required for this response, we were interested in adapting the procedure to a screening technique for antiphlogistic agents. This necessitated some changes in the technical details of the original method. Because of the success of this revised method during the last two years, numerous requests have been received for an outline of the procedure. An essential requisite for consistent results is the strict adherence to minute detail in technique. Accordingly, such details are described in this report.
A polarographic assay for khellin in serum has been developed. The method has been applied to in vivo studies and has been found adequate to show the presence of khellin in small amounts (0–10 μg./ml.). The average recovery of khellin was 90 per cent, the precision of the method varying with the blood level. It was ±5 per cent at a level of 10 μg./ml. and was ±40 per cent at 1 μg./ ml.
