The urinary catecholamine metabolites, vanimandelic acid, homovanillic acid, 3,4-dihydroxyphenylacetic acid and 5-hydroxyindoleacetic acid, were extracted on a silica-bonded strong-anion-exchanger cartridge (SAX) and then injected into an high-performance liquid chromatographic (HPLC) system by column switching. Chromatography was performed on a reversed-phase analytical column with electrochemical detection. Full automation was obtained by coupling two devices: a solid-phase automatic sampler and intelligent autosampler. For each substance the recovery was greater than 95% and the coefficient of variation was ca. 3%; the analysis takes 11 min. Substance instability problems are overcome, because the samples are extracted and injected in rapid succession. The normal values and correlation with manual HPLC were established for a large number of samples.
A fully automated analyzer is described for the HPLC analysis of catecholamines.
R Battistoni合作论文数11G Grossi合作论文数9Alberto Bargossi合作论文数4
Carlo Sturani合作论文数3L Comellini合作论文数2G Sprovieri合作论文数2Gabriele Grossi合作论文数2Gp Salvioli合作论文数2Ms Morini合作论文数2Fabrizio Sandri合作论文数2E Mule合作论文数1T Lubich合作论文数1S Cassarani合作论文数1Gp Bianchi合作论文数1Ga Checchia合作论文数1renato calliva合作论文数1M Zoli合作论文数1Enrico Strocchi合作论文数1
Giulio Marchesini Reggiani合作论文数Dipartimento di Medicina Interna e Gastroenterologia, “Alma Mater Studiorum”, Università di Bologna1
Maurizio Battino合作论文数Department of Specialised Clinical Sciences and Odontostomatology, Polytechnic University of Marche;Dept Clinical Sciences, Università Politecnica delle Marche;Centre for Health and Nutrition, Universidad Europea del Atlantico;Jiangsu University1
Spyros Papiris合作论文数2nd Pulmonary Medicine Department, School of Medicine, National and Kapodistrian University of Athens;"ATTIKON" University Hospital1Virginia L. Wyss合作论文数1
Stefano Boschi合作论文数1AM Bargossi合作论文数1g p ganzit合作论文数1PL Fiorella合作论文数1
