The study evaluated fatteners produced by crossing DanBred Yorkshire-Landrace (YL) sows with purebred Landrace, Pietrain, and Duroc boars from Topigs Norsvin. Approximately 200 pigs were assessed per crossbred group. From each group, 60 carcasses were analysed for lean meat content using the AutoFom device, and longissimus lumborum (LL) muscles from 30 carcasses were collected for meat quality evaluation. Pigs sired by Duroc boars exhibited significantly higher average daily gain (ADG) than Pietrain-sired pigs, while feed intake and feed conversion efficiency did not differ among groups. Carcasses from Landrace- and Pietrain-sired pigs contained more lean meat than those from Duroc-sired pigs. Across individual cuts (ham, loin, shoulder, and belly), Pietrain-sired pigs showed the highest lean meat content, followed by Landrace, with the lowest values observed in the Duroc group. Meat quality traits were most favourable in Duroc-sired pigs, as indicated by higher pH values and lower drip and thawing losses in both fresh and frozen meat. Their LL muscles also contained higher dry matter and intramuscular fat (IMF) levels compared with those of Landraceand Pietrain-sired pigs. These attributes translated into superior sensory quality, reflected in higher scores for aroma, tenderness, juiciness, and flavour, as well as lower shear force values.

This research aimed to assess the impact of replacing a partially of post-extraction soybean meal in the diet with varying amounts of distillers yeast (3, 6 and 9%) on the composition and quality in the pectoral muscles of broiler chickens. Findings revealed that cockerels fed with 3% yeast exhibited elevated oleic acid levels and reduced n-6 fatty acids compared to those fed with 6% and 9% yeast. Furthermore, chickens consuming 3% yeast displayed higher antioxidant capacity (ABTS) and decreased levels of linoleic acid and its ratio to α-linolenic acid compared to the 9% yeast group. Moreover, muscles from cockerels on the 3% yeast diet and the control group demonstrated higher shear force, lower n-6/n-3 ratio and lipid peroxidation rate (TBARS) than those on the 9% yeast regimen. Conversely, cockerels on the 9% yeast diet exhibited reduced gumminess and ferric reducing antioxidant power (FRAP) compared to the control group. The study highlights yeast’s role in altering broiler chicken meat’s fatty acid profile, texture, and antioxidant properties.

The study was conducted on 112 broiler chickens fed for 35 and 37 days with various levels of distiller's yeast in diets - 3% in starter, 6% in grower, and 9% in finisher feeds. The results showed that as the level of distiller's dried yeast in the broiler diets increased, the total number of bacteria in the mucosa of the small intestine, cecum, and colon decreased, including mainly Enterobacteriaceae, Enterococcus, and E. coli. The addition of 9% yeast to the diet significantly reduced the abundance of Lactobacillus sp. in the intestinal mucosa of broiler chickens. There was no significant effect of the level of distiller's dried yeast in the diet of broiler chickens on carcass quality, pH, water holding capacity, sensory characteristics, and macronutrient and micronutrient content in breast muscle. On the other hand, there was a significant effect of the analyzed distiller's yeast on color characteristics and basic chemical composition of breast muscle. The highest lightness (L*), yellowness (b*), and chroma (C*) and the lowest dry matter content were found in the meat of chickens receiving 6% and 9% distiller's dried yeast in their diets. The results of the study indicate that for the best results in terms of carcass and meat quality, the optimal content of the analyzed yeast in the diet of broiler chickens should not exceed 3%.

The study aimed to assess the effect of supplementation of pig diet with the Bokashi probiotic on the fatty acid profile of longissimus lumborum (LL) muscles and backfat. The research involved 120 hybrid pigs deriving from Naïma sows and P-76 boars. The experimental group’s pigs received probiotics in their feed (containing Saccharomyces cerevisiae, Lactobacillus casei, and Lactobacillus plantarum). To analyze the fatty acid profile in intramuscular fat (IMF) of LL and backfat, 24 pig carcasses from the control group and 26 from the probiotic-supplemented group were randomly selected. Probiotic supplementation increased the Atherogenic Index, reduced the proportion of C20:4, and increased C12:0 and C18:2 n-6 in IMF LL, without affecting ΣSFA, ΣMUFA, and ΣPUFA. In backfat, probiotic supplementation decreased C18:1 and C18:2 n-6 proportion and increased C18:3 n-3, C20:3 n-6, and C20:4 n-6. These changes resulted in significantly higher ΣMUFA, ΣPUFA, PUFA Σn-3/Σn-6, and lower Saturation Index (SI). From a consumer health and technological point of view, probiotic supplementation improved the lipid profile of backfat to a greater extent than LL muscle. Bokashi, at a dose of 3 g/kg of feed in the last stage of pig production, had no significant effect on the fatty acid profile of the meat.

The aim of this research was to assess the influence of partially substituting post-extraction soybean meal with distillers dried yeast on the efficiency of broiler chicken fattening and the intestinal microflora.I Inactive distillers dried yeast Saccharomyces cerevisiae, cultivated on wheat extract, was employed in the study. The research was conducted on 112 Ross 308 line cockerels subjected to a 35-day fattening period, during which they were fed Starter (days 1–10), Grower (days 11–27), and Finisher (days 28–35) diets. Broiler chickens were randomly divided into four groups: control and three experimental groups with varying levels of distillers dried yeast—3%, 6%, and 9%. Cockerels in the control group (Starter diet) exhibited higher feed consumption compared to those receiving 3% yeast in their diet. Cockerels in the group receiving 3% yeast (Grower diet) demonstrated better feed conversion compared to those receiving 9% yeast in their diet. The study revealed that regardless of the broiler chicken fattening period, there was a decrease in the overall number of isolated microorganisms in the feces with an increase in the proportion of distillers dried yeast in the diet. This was confirmed by the total number of bacteria, anaerobic bacteria, Enterobacteriaceae, E. coli, Enterococcus sp., and S. aureus. Based on cluster analysis (dendrogram), it was observed that only a 9% addition of yeast to the diet significantly influenced the quantitative and qualitative composition of the fecal microbiota of broiler chickens. In summary, a properly balanced feed with distillery yeast can be used as a good source of energy and protein in feed mixtures for broilers, serving as a cost-effective alternative to soybean meal. However, for optimal production results and feed costs, the inclusion of yeast in the feed should not exceed 3%.

For a large number of consumers today, primarily young ones, acceptance of a foodstuff such as meat is often linked to demands for appropriate conditions for the maintenance of productive animals, as well as proper protective treatment during transport and slaughter. The way in which commercial and slaughter animals are handled is being viewed more and more critically by the public. The increasing sensitivity of consumers to the fate of animals in recent years is also reflected in the emergence of new legislation. The quality of pork meat largely depends on how the animals are handled, and in particular on factors related to pre-slaughter marketing and the conditions of slaughter itself. During pre-slaughter marketing, the body of fattening animals and the quality of pork are particularly adversely affected by conditions during loading, transport, holding in the livestock warehouse, and slaughter. Carried out even under the best conditions, they induce a severe stress for pigs, resulting in significant reduction in the quantity and quality of meat.

Purpose The objective of this study is to assess whether pro-health herbal probiotics, ascorbic acid and allicin added to the finishing diets of hybrid pig influenced the intramuscular fat (IMF) content in longissimus lumborum (LL) muscle, the fatty acid profile and lipid quality indices, as it has an impact on human health. Design/methodology/approach After 80 days of equal fattening, the pigs were divided into the control group (CT, n = 30 received commercially allowed and applicable antibiotics) and the experimental group (EX, n = 30), which until 95 days of rearing were supplied with fermented herbs extract (FHE Multikraft® Austria) with probiotics Saccharomyces cerevisiae , Lactobacillus casei , L actobacillus plantarum , L-ascorbic acid and extract of garlic (10% allicin). After slaughter, crude fat content and fatty acid profile were determined in LL muscle samples, and on that basis lipid indices were calculated. Findings Supplementation with FHE, probiotics, L-ascorbic acid and allicin has significantly impacted the crude fat content in the meat and the percentage of fatty acids content: tricosanoic (C23:0), heptadecanoid (C17:1 n -7), eicosanic (C22:1 11cis n -9) and eicosatrienoic (C20:3 11cis n -3), in comparison to CT group. Amongst lipid quality indices, IMF in LL of pigs from EX group, the C18:2 n -6/C18:3 n -3 ratio is characterised by a significantly higher value and thus is more beneficial to the health of the consumer. Originality/value The authors have indicated that using FHE, probiotic supplements with ascorbic acid and allicin in commercial fattening of pigs, as an alternative for antibiotic growth promoters (AGP), improves the fatty acid profile of the meat.

Ross 308 chickens were used to investigate fatty acid (FA) composition and oxidative stability of broiler chicken meat following the controlled inhibition of peroxidation in feed containing a concentrated source of omega-3 fatty acids (flaxseed oil, FLO), approximately 50% omega-3. Ninety-six one-day-old chicks were randomly allocated to four dietary treatments (n = 24/group) that included rapeseed oil (RO), flaxseed oil (FLO), RO with optimised quercetin (Q) - RO_Q, or FLO with optimised Q (FLO_Q). On day 35, breast and thigh muscles were collected in order to analyse their FA profile and malondialdehyde (MDA) levels. Dietary treatments had no effect on weight gain or feed conversion ratio in chickens. However, dietary FLO increased the deposition of α-linolenic acid in both pectoral and thigh meat (P < 0.05), and tended to decrease the ratio of omega-6/omega-3 FA in pectoral muscles (P < 0.07). Addition of an optimised concentration of Q proved to be an efficient way of limiting lipoperoxidation in breast and thigh muscles subjected to refrigeration at 2-3 °C for either 1 or 7 days. Results were consistent with the observed inhibition of peroxidation in feed mixtures and significantly correlated with MDA levels found in feed mixtures. These results provide evidence that it is possible to produce poultry meat with an improved proportion of omega-3 FA without significantly altering the performance of broiler chickens or the oxidative stability of their meat.

This study analyzed the effect of finishing diet supplementation with herbal probiotic preparation, ascorbic acid, and allicin on mortality rates of pigs, and longissimus lumborum (LL) and semimembranosus (SM) muscle quality, including the levels of cholesterol, macroelements and microelements. The experiment was carried out on 3614 hybrid pigs (equal share of gilts and barrows), of the offspring of PIC410 boars and DanBred sows (Landrace-Yorkshire), divided into two groups. The experimental group of pigs received Fermented Herbs Extract (Multikraft, Austria) enriched with probiotics (S. cerevisiae, L. casei, L. plantarum) in addition to ascorbic acid (E300) and allicin during the finishing period (95 days), while the control group was fed a typical finishing diet containing levels of antibiotics consistent with those used in commercial pig production. Our results showed a positive effect of supplementation with herbal probiotics, allicin and ascorbic acid in lowering finishing pig mortality and increasing pork quality as evidenced by higher pH (40 min post mortem in the SM muscle; 24 h and 48 h in the LL muscle), lower thaw loss, higher dry matter and intramuscular fat (IMF), and higher yellowness (b*) and chroma (C*) while maintaining similar carcass lean/fat content. However, results showed no effect of the supplements on the levels of cholesterol, macroelements, and microelements in the LL muscle, except for Mn, whose concentration was higher in the control group. Compared to barrows, the carcasses of gilts were characterized by a greater share of lean meat and lower backfat thickness, with similar physicochemical and functional characteristics of meat. The results also showed interactions between the groups of pigs (control and supplementation) and their sex in shaping L* and the content of ash, potassium and phosphorus in the muscles. In summary, from the point of view of production economics, the use of the analyzed supplements in the finishing feed and through fogging resulted in better health (no need for the use of antibiotics) and lower mortality of pigs, which ultimately resulted in better financial results. In addition, the use of these additives in pig fattening had a beneficial effect on meat quality.

Abstract The primary aim of this study was to estimate the relationship between ATP citrate lyase (ACLY) gene polymorphism (c.*523 T>C) and fattening and pork quality traits. Investigations were carried out on 526 pigs represented by three breeds: Polish Landrace (n=269), Polish Large White (n=189) and Puławska (n=68). ACLY genotypes were determined by PCR–RFLP method. It was demonstrated that the analyzed polymorphism had significant influence (P<0.05 and P≤0.01) on several economically important traits in pigs, e.g. average daily gain, average backfat thickness, lean meat percentage. The results obtained allow for application of c.*523 T>C polymorphism in breeding programs to improve the pig population in terms of fattening and slaughter traits. However, this breeding program may have a slight negative effect on meat texture parameters.

The aim of this study was to determine the effect of probiotics on gut microbiota, on carcass and meat quality and on mineral contents in the longissimus lumborum (LL) muscle in pigs. The research was carried out with 120 hybrid pigs deriving from Naïma sows and P-76 boars. Pigs from the experimental group received the EM®Bokashi probiotic (Greendland Technologia EM®, Janowiec n/Wisłą, Poland) in their feed (containing Saccharomyces cerevisiae, Lactobacillus casei and Lactobacillus plantarum). The study showed that EM®Bokashi probiotic supplementation resulted in a significantly higher count of lactic acid bacteria (LAB) and yeasts in the feed, a lower number of Clostridium in the mucosa and colorectal digesta as well as a lower Enterobacteriaceae count in the colorectal digesta. The research showed that carcasses of the pigs who received the EM®Bokashi probiotic had a higher lean percentage and lower fat content than the carcasses of the control fatteners. Diet supplementation with the EM®Bokashi probiotic resulted in a lower pH and technological yield (TY) and a higher drip loss and shear force at a lower protein content in LL muscle. Moreover, the administration of the probiotic to fatteners resulted in higher yellowness (b*) and saturation (C*) and higher concentrations of Na, Mg and Se in meat.

The objective of this study was to conduct a preliminary assessment of the impact of adding salutogenic formulations to turkey feeding diets on the fatty acid profile of intramuscular fat from breast and on their lipid profile indicators. The experiment was carried out on 60 BIG-6 breed turkeys. Turkey toms were fattened up to 20 weeks of age (140 days). From the rearing day of 42, the turkeys in the control group (BIOPOINT) were given Biopoint (R) supplements in drinking water (copper, selenium, flavonoid, lysine, vitamins C and E, garlic extract), while the experimental group was given Multicraft. fermented herbs extract (FHE) containing Saccharomyces cerevisiae, L. casei, L. plantarum. Twelve hours post mortem, m. pectoralis superficialis was excised and used for sampling (n=30 for each group), then stored for 18 months at the temp. of -20 degrees C. 12 samples from or each group were taken for analysis in this pilot study. Once the samples were defrosted, the lipid profile was evaluated with the use of gas chromatography. The total antioxidant capacity of meat was determined by using the FRAP method. Based on FA contents, the following indexes were calculated: atherogenicity (AGI), thrombogenicity (TI), polyene peroxidisability (PI), nutri value (NVI) and h/H ratio. Regarding consumers' health, the lipids from FHE group of turkeys were characterized by a more expedient lipid acids profile and the following indicators: P/S (1.32), TI (0.63), h/H (1.41), PI (42.1), polygene index (0.06), compared with those from BIOPOINT group turkeys (vs. 1.17; 0.67; 1.26; 0.0; 38.3). Supplementation with FHE had a positive impact on the total antioxidant status of the turkey muscles. It may be concluded that owing to its positive influence on the metabolism of lipids and lipoproteins, the breast meat sourced from turkeys fattened with FHE supplementation with a confirmed salutogenic lipid profile may be beneficial for the health of consumers in the context of prevention of cardiovascular diseases.

The objective of this study was to assess how probiotic bacteria added to diets of 60 hybrid barrows (Landrace-Yorkshire x Duroc) affected the basic chemical composition of meat, the fatty acid profile of intramuscular fat (IMF), m. longissimus lumborum (LL) and lipid quality indices, because IMF cannot be removed before consumption and this inevitably has an impact on human health. After 78 days, the pigs were divided into the control group (CT, n=30) and the experimental group (BP, n=30), which until 97 days of rearing were supplied with BioPlus YC probiotic (Bacillus licheniformis DSM 5749 and Bacillus subtilis DSM 5750). After slaughter, 12 carcasses of similar weight (90 +/- 5 kg) from each group were selected for testing. Supplementation with probiotic Bacillus did not affect the basic chemical composition of meat. It was established that BioPlus YC had a significant impact on capric - C10:0 (0.08 vs. 0.13), eicosatrienoic - C20:3 n-3 (1.76 vs. 2.14) and SPUFA n- 3 acids (1.88 vs. 2.41), causing a reduction in their percentage, which was thereby different than in the CT group. Among lipid quality indices, IMF LL of pigs from the BP group, the thrombogenicity index (TI) was characterized by a significantly (P <= 0.01) higher value than in the CT group (1.08 vs. 0.99). These results suggest that further research is needed to study differences in compounds affecting the fatty acid profile of pork, and to confirm the association of the lipid profile with the use of BioPlus YC probiotic in pig fattening.

The study showed that 0.3% and 0.5% EM (R) probiotics supplementation resulted in a significantly higher count of lactic acid bacteria (LAB) in the feed, lower ADG (average daily gain) in the last four weeks before slaughter, along with higher electrical conductivity (EC2), higher cooking loss, and lower juiciness of the longissimus lumborum (LL) muscle compared to the control group. The 0.5% supplemented group had a higher count of LAB in the mucosa, and a lower number of Enterobacteriaceae in the mucosa and colorectal content, as well as a higher EC24, higher drip and thaw loss, higher shear force and lower score in the color assessment of cooked meat, compared to the 0.3% and control groups, along with the highest redness (a*), yellowness (b*) and chroma (C*), and the lowest hue angle (h(o)).

Background: Growth ability and meat performance are of great economic importance in beef cattle. Therefore, breed comparison experiments are needed to provide valuable information on the suitability of different beef breeds for different production conditions. The aim of the present study was to compare the growth performance and meat quality of young Red Angus, Salers and Limousin bulls. Methods: The material comprised 30 bulls of Red Angus (RA), Salers (SL) and Limousin (LM) breeds. The parameters studied were 210-d bodyweight and daily gains from birth to weaning and to finishing, as well as from weaning to finishing. The finished bulls were slaughtered after 12-hour fasting and post-slaughter evaluation was carried out. The compositions of the samples of meat from the longest dorsal muscle (m. longissimus dorsi) were subjected to basic chemical analysis and sensory analysis. Result: The best results in the period of maternal nursing were obtained by the SL bulls and during intensive fattening, by the LM bulls. The differences existed in the chemical composition and sensory characteristics of meat from the bulls of the studied breeds. The RA breed demonstrated the highest content of intramuscular fat in the longissimus dorsi muscle and received higher scores for sensory characteristics.

The objective of this study was to determine carcass value produced by PIC359 boars' progeny when crossed with DanAvl Hybrid (landrace-york-shire) sows; technological and sensory quality of m. longissimus lumborum muscle was also evaluated. The studies were carried out on DanAvl Hybrid (LY) and LY x PIC359 fatteners. Studies have shown that LY x PIC359 hybrids were characterized by better leanness and lower fatness of carcasses as well as higher redness (a*), yellowness (b*) and saturation (C*) of meat color in relation to fatteners from the LY group. The influence of PIC359 boars on technological quality of meat was not demonstrated, as evidenced by pH (35 min - 96 h), electrical conductivity (2 and 24 h), water binding characteristics of raw and frozen meat and lightness (L*). In addition, there were no differences between the analyzed groups of fatteners in basic chemical composition of meat, its sensory characteristics and shear force, with the exception of flavour, that was significantly better in LY x PIC359 hybrids compared to LY. In summary, it can be pointed out that cross-breeding PIC359 boars with LY sows positively influenced both the quality of their carcass and meat in the progeny.

The objective of the study was to determine the effects of probiotic bacteria Bacillus licheniformis and Bacillus subtilis on microbiological properties of feed mixtures and on the digestive tract content as applicable to production traits and carcass characteristics of fatteners. The experiment was performed on 83,838 fatteners from four successive (insertions) productions in two groups. From the seventy eighth day of age till marketing to the slaughter plant, the pigs were supplied with BioPlus YC probiotic (Chr. Hansen) in the amount of 400 g/t. The preparation contained a complex of probiotic bacteria Bacillus licheniformis DSM 5749, and Bacillus subtilis DSM 5750 spores in a 1:1 ratio. From the fourth insertion, after reaching a body weight of approximately 112 kg, 60 fatteners were selected from each group to measure carcass quality and half of them for meat quality evaluation. Moreover, microbiological analyses in feed and colon were performed. The study showed that BioPlus YC probiotics supplementation resulted in a significantly higher count of B. subtilis and B. licheniformis in the feed, a higher count of B. subtilis, B. licheniformis and LAB, as well as a lower count of Enterobacteriaceae, Enterococcus, Clostridium and Bacillus sp. in the mucosa and in the colorectal content of the test pigs. Our work has shown that supplementation with the BioPlus YC probiotic had a positive effect on the production traits of pigs mainly by reducing mortality (2.83%, p = 0.010), lowering feed conversion ratio-FCR (2.59 kg/kg, p = 0.013), better average daily gain-ADG (0.95 kg/day, p = 0.002) and shorter fattening period (77.25 days, p = 0.019) when compared to the control group (4.19%; 2.79 kg/kg; 0.89 kg/day; 92.8 days, respectively). The addition of the specific Bacillus bacteria did not influence carcass and meat characteristics of the test fatteners.

The research was aimed at determining the physicochemical and sensory properties of the m. longissimus lumborum at DanAvl Hybrid (Landrace – Yorkshire) fatteners and derived from DanAvl Hybrid sows and DanAvl Duroc and Pulawska boars. Fatteners derived from DanAvl Duroc boars were characterized by better meat quality compared to DanAvl Hybrids and their hybrids with Pulawska breed as evidenced by higher pH in 24–96 h post mortem (p.m.), higher sensory evaluation of juiciness, tenderness and flavor as well as lower shear force at lower content of total protein and higher content of intramuscular fat (IMF). However, DanAvl Hybrid fatteners were characterized by poorer meat quality, i.e. higher color lightness (L*) and higher drip loss both from fresh and thawed meat compared to those derived from DanAvl Duroc and Pulawska boars. Meat of fatteners after Pulawska breed was characterized by the highest redness (a*), and that after DanAvl Duroc breed, the lowest yellowness (b*) and saturation (C*).

The research was aimed at determining the physicochemical and sensory properties of the m. longissimus lumborum at DanAvl Hybrid (Landrace – Yorkshire) fatteners and derived from DanAvl Hybrid sows and DanAvl Duroc and Pulawska boars. Fatteners derived from DanAvl Duroc boars were characterized by better meat quality compared to DanAvl Hybrids and their hybrids with Pulawska breed as evidenced by higher pH in 24–96 h post mortem (p.m.), higher sensory evaluation of juiciness, tenderness and flavor as well as lower shear force at lower content of total protein and higher content of intramuscular fat (IMF). However, DanAvl Hybrid fatteners were characterized by poorer meat quality, i.e. higher color lightness (L*) and higher drip loss both from fresh and thawed meat compared to those derived from DanAvl Duroc and Pulawska boars. Meat of fatteners after Pulawska breed was characterized by the highest redness (a*), and that after DanAvl Duroc breed, the lowest yellowness (b*) and saturation (C*).

The study was conducted on BIG-6 heavy white turkey toms. A control group received standard vitamin and mineral supplements under the Biopoint brand (Cardiox, Eselen, Turboalistar, wit. C) during fattening. An experimental group of turkeys received Fermented Herbs Extract (FHE) enriched with Effective Microorganisms (Saccharomyces cerevisiae, Lactobacillus casei, Lactobacillus plantarum). Meat quality and basic chemical composition were analyzed on pectoralis superficialis (PS) muscle. Better meat quality was found in the FHE group, as shown by a higher pH(24), and water holding capacity (WHC); lower electrical conductivity (EC20min) and lightness (L*). However, turkeys supplemented with additives under the Biopoint brand had a higher content of dry matter, including total protein in meat. Supplementation with pro-health additives did neither affect carcass slaughter value, sensory characteristics nor shear force.