The purpose of this study was to investigate the efficacy of antiseptics meatal care in preventing catheter-related urinary tract infections (UTIs) in patients with an indwelling urinary catheter in the intensive care unit of Osmangazi University Medical School. One hundred patients were divided into four groups (25 per group) and treated with once or twice daily application of chlorhexidine gluconate or povidone–iodine. A control group was also studied (N=30). Urine samples were taken weekly and cultures were evaluated quantitatively. Meatal swabs were obtained on the first, fifth, and 10th day and determinated semiquantitatively. UTI was defined as bacteriuria with 105 cfu/L. Cultures showing no growth or mixed growths were stated as negative for UTI. UTI developed in 16 patients on days two, three, four, five and seven (including control group). Dominant micro-organisms in the meatal area were found to be Candida species. In nine cases the causative agents of UTI were Candida species. It was therefore decided that the use of antiseptics to clean the periurethral area provides no benefit in decreasing the rate of bacteriuria.

A simple, precise, rapid, and accurate reversed-phase high performance liquid chromatographic (RP-HPLC) method has been developed for the determination of sildenafil citrate (SLD) in pharmaceutical dosage forms and spiked human plasma. Chromatography was carried out on a C-18 reversed-phase column, using a mixture of acetonitrile:water(45:55, v/v) as a mobile phase at a flow rate of 1 mL . min(-1). Phenobarbital sodium was used as an internal standard (IS) and detected using a diode array detector at 220 nm. Retention times of SLD and IS were 7.2 and 3.2 min, respectively. The linear range of SLD was found to be 5 x 10(-8)-1 X 10(-5)mol . L-1. Limit of quantitation (LOQ) and limit of detection (LOD) were calculated to be 7.5 x 10(-8) and 2.2 x 10(-8) mol . L-1, respectively. The method was validated for its linearity, precision, accuracy, stability, robustness, and ruggedness. The proposed method was applied to sildenafil tablets and spiked human plasma. In addition, the results were compared with those obtained from UV-spectrophotometry.