The Paraguayan Chaco has experienced, in the last few decades, some of the highest rates of deforestation in the world. In parallel, this region has registered an increase in the number of cattle heads of 60% in the last decade. Taking into account the high environmental and socioeconomic impact of this expansion, the aim of this work was to reveal how Beef–cattle ranching is carried out and to establish a typology that allows us to identify the different land-use patterns followed by the ranches. Data were collected using face-to-face structured interviews of 80 ranch owners. In the region ranches co-exist that practise the cow–calf system, the whole-cycle system and the fattening system. In all cases, ranches are very large, pasture based, highly specialised in Beef–cattle and export-oriented. Three groups of ranches were identified, being the main differentiating drivers: (i) the availability of the different production factors, (ii) the distribution of total area, and (iii) the degree of intensification in the use of capital, labour and/or technology per unit of agricultural area. In addition, it is noted that the years of activity of the ranches are related to these drivers. The typology of ranches contributes to a better understanding of one of the most active livestock frontiers in the world and shows that the expansion process taking place in the Paraguayan Chaco is associated with an intensification of Beef–cattle systems. These results provide a useful approach to develop policies that regulate the expansion of the cattle frontier in the Paraguayan Chaco.

In this paper, we present the simulation results of a Lagrangian particle tracking model that computes the motion of saltating sediment particles, which is considered the most important mode of bedload transport in rivers and channels. The model is one-way coupled to a validated turbulent LES-WALE (Large Eddy Simulation – Wall-Adapting Local Eddy-viscosity) channel flow, i.e., the particles do not affect the computation of the flow velocities and pressures, as suggested for dilute flows. The model addresses the particle trajectories, the collision of the particles with the bottom wall, and collision among particles. The focus of this work is placed on the effect of different particle concentrations and flow intensities (different flow shear stresses) on jump statistics and particle diffusion. Numerical results are validated with experimental laboratory data obtained from the literature for particle diameters in the range of sands. The present results indicate that, at particle concentrations up to 2%, the diffusion coefficients in the streamwise and spanwise directions, γx and γz, for the local range are nearly constants with a value close to one, corresponding to the ballistic regime. At a concentration of 4%, the largest concentration studied herein, values of γx and γz for the local range are slightly smaller, with a representative value of 0.9 regardless of flow intensities. For the intermediate regime, it was found that, on average, γx~1.2γz with γx ranging from 0.6 to 0.85 and γz within the range 0.45–0.70. For a fixed flow intensity, both diffusion coefficients increase with the particle concentration, which is an indication of the contribution of the collision among particles to particle diffusion. For highly controlled simulation conditions, the differences in particle velocity at a given concentration may change drastically, which should translate to important fluctuations in the computation of sediment transport rates. Finally, the employed computational resources are described as a function of particle concentration. Although the number of total collisions increases linearly with the number of particles, the number of collisions per particle reaches a plateau, thus indicating that there exists an upper limiting value for the number of collisions per particle.

The composition of extracellular vesicles (EVs) is altered in many pathological conditions, and their molecular content provides essential information on features of parent cells and mechanisms of crosstalk between cells and organs. Metabolic Syndrome (MetS) is a cluster of clinical manifestations including obesity, insulin resistance, dyslipidemia and hypertension that increases the risk of cardiovascular disease and type 2 diabetes mellitus. Here, we investigated the crosstalk between liver and adipocytes by characterizing EVs secreted by primary hepatocytes isolated from Zucker rat model, and studied the effect they have on 3T3-L1 adipocytes. We found that steatotic hepatocytes secrete EVs with significantly reduced exosomal markers in comparison with their lean counterpart. Moreover, proteomic analysis revealed that those EVs reflect the metabolic state of the parent cell in that the majority of proteins upregulated relate to fat metabolism, fatty acid synthesis, glycolysis, and pentose phosphate pathway. In addition, hepatocytes-secreted EVs influenced lipolysis and insulin sensitivity in recipient 3T3-L1 adipocytes. Untargeted metabolomic analysis detected alterations in different adipocyte metabolic pathways in cells treated with hepatic EVs. In summary, our work showed that steatosis has a significant impact in the amount and composition of EVs secreted by hepatocytes. Moreover, our data point to the involvement of hepatic-EVs in the development of pathologies associated with MetS.

El principal objetivo de esta investigación es determinar el efecto de altas concentraciones de gases y material particulado de vehículos diésel sobre el rendimiento cuántico máximo del fotosistema II (Fv/FmPD) a pre-alba de la especie arbórea Quillaja saponaria Mol. (Quillay), la especie arbórea nativa más utilizada en zonas urbanas de Santiago, Chile. Se examinaron dosis de riego en individuos jóvenes de Q. saponaria en una cámara de crecimiento. Los resultados muestran que las plantas con riego restringido (-30 bares en promedio) resisten mejor el material particulado grueso y presentan valores de Fv/FmPD significativamente mayores que las plantas regadas (-8 bares en promedio). Las emisiones de monóxido de carbono, material particulado fino y ozono redujeron significativamente el Fv/FmPD en las plantas con irrigación, incluidas las plantas con riego restringido. Se concluye que las plantas Q. saponaria regadas presentan una tendencia a ser más afectada en el Fv/FmPD por dichos contaminantes que las plantas con riego restringido.

Abstract Study question Is it possible to use free and extracellular vesicle-associated microRNAs from endometrial fluid as non-invasive biomarkers for implantative endometrium? Summary answer It is feasible to use free and extracellular vesicle-associated miRNAs as non-invasive tools for the detection of an implantative endometrium. What is known already MicroRNAs (miRNAs) and extracellular vesicles (EVs) from endometrial fluid (EF) have been described as mediators of embryo-endometrial crosstalk. Therefore, the miRNA analysis of EF could provide a non-invasive technique for recognizing an implantative endometrium and consequently improve implantation rates. Study design, size, duration A cohort of 162 women who assisted the human reproduction unit from January 2018 to February 2021. Of them, 72 participated in the setup and samples were collected before starting any fertility treatment in natural cycles. For the discovery of the predicted models (n = 30) and validation (n = 60), the EF was obtained from women undergoing frozen embryo transfer on day 5 and the sample was collected immediately before embryo transfer. Participants/materials, setting, methods We compared five different methodologies, two of which consisted of direct RNA extraction while the other three had EV enrichment prior to RNA extraction. A smallRNA-seq was performed to determine the most efficient method and to find a predictive model to differentiate between implantation and non-implantation endometrium. The models were confirmed by qPCR in two set of samples, discovery and validation cohorts with a different implantation outcome. Main results and the role of chance Our results showed that EV-enrichment protocols permit detection of a higher number of miRNAs. In addition, we obtained two predictive models based on three miRNAs that allow us to differentiate between an implantative and non-implantative endometrium. Results for model 1 in discovery cohort were: AUC=0.93; p-value = 0.003 and in validation cohort were: AUC= 0.69; p-value = 0.019. Results for model 2 in discovery cohort were: AUC=0.92; p-value = 0.0002 and in validation cohort were: AUC= 0.78; p-value= 0.0002. Limitations, reasons for caution One limitation to consider is the inherent variability of both the women involved in the trial and the embryos transferred. In our study, we have previously selected embryos based on morphology but we lacked genetic and molecular studies, a good complement that will certainly improve our test’s accuracy. Wider implications of the findings This study introduces new protocols to analyze miRNAs from very small volumes of EF, which could be implemented in clinical practice for the assessment of the endometrial status using miRNA-based non-invasive tools. Our results suggest that with model-2 it is possible to identify a non-implantative endometrium with 0.6-sensitivity and 0.93-specificity. Trial registration number not applicable

We describe a new locality with ctenochasmatid pterosaurs found in a tidal estuarine paleoenvironment of the Quebrada Monardes Formation (Lower Cretaceous). The new locality, which is named "Cerro Tormento", is in Cerros Bravos in the northeast Atacama region, Northern Chile. Here, we describe four cervical vertebrae, one of them belonging to a small individual, the impression of a right scapulocoracoid, a left coracoid, an impression of a left humerus, an incomplete left humerus, a distal fragment of the right humerus, and impressions of a left femur and tibiotarsus. The presence of three humeri and a cervical vertebra belonging to a small pterosaur indicate that these materials represent more than one individual. The cervical vertebrae present diagnostic traits shared with ctenochasmatid pterosaurs, such as elongated vertebral centra, with integrated neural arch, low neural spines, and dorsally located neural canal. It is currently not possible to determine if there are one or more species represented. This finding is the second geographic occurrence of pterosaurs of the clade Ctenochasmatidae in the Atacama region, although it is currently uncertain if ctenochasmatids from both locations were contemporaneous. This suggests that the clade Ctenochasmatidae was widespread in what is now northern Chile. In addition, the presence of bones belonging to more than one individual preserved in Cerro Tormento suggest that pterosaur colonies were present at the southwestern margin of Gondwana during the Early Cretaceous.

The traditional production of the Iberian breed pig involves a long production cycle. It might be shortened by using Iberian pigs crossed with Duroc and by reducing the growing phase, but the age-related changes on productive performance and carcass quality should be addressed. Thus, productive performance, live measurements and carcass and primal cut traits were evaluated on Iberian × Duroc 50:50 crossed pigs according to animal age at the beginning of the free-range finishing phase (Montanera): 10, 12 and 14 months old (IBxD10 (n = 15), IBxD12 (n = 17) and IBxD14 (n = 18) animal batches, respectively) and gender (immunologically castrated female -consisted of the Improvac® vaccination- and surgically castrated males). During the growing period, animals were fed with restrictions; 1.49, 1.29 and 1.20 ± 0.023 (mean ± SEM) kg/day of commercial feeds to start Montanera with similar BW; 103.9, 102.9 and 102.1 ± 0.22 kg, for IBxD10, IBxD12 and IBxD14, respectively. IBxD14 animals yielded the highest average daily gain (ADG) and BW after Montanera, as well as larger rump height and croup width. In contrast, these animals had the lowest carcass yield. Although animals from IBxD10 yielded hams of inferior size, this could be of interest to the sector, as there is a certain segment of the market that demands hams of smaller size and, generally, this is difficult to obtain with the traditional Montanera production system. The gender had no major effects on performance and carcass and primal cut traits, so both immunologically castrated female and surgically castrated males are suitable for finishing in Montanera.

Delgado, Maria Cruz Martín; Gonzalez, Elena; Rodado, Isabel; Camino, Nuria; Muñoz, Carlos; Gómez, Eva; Riesco, Laura; Estévez, Maria Angeles; Cordoba, Luis; Stanescu, Dennis; Navarro, Gonzalo; Pavalascu, Amelia; Moliner, Noelia; Algaba, Ángela; Abril, Elena Author Information

Se presenta un caso de sarcoidosis que presentó afectación suprarrenal. Hombre de 44 años fue diagnosticado de sarcoidosis pulmonar y ganglionar según hallazgos clínicos, laboratoriales y anatomopatológicos. Tres años después acude por debilidad generalizada, náuseas, hiponatremia persistente, hipotensión arterial, pérdida de peso e hiperpigmentación cutánea. La evaluación endocrinológica confirma insuficiencia suprarrenal primaria, lo que sugiere la infiltración de la sarcoidosis en las glándulas suprarrenales.

Introducción: La adherencia a las medidas de bioseguridad por el personal de salud es una estricta necesidad en los tiempos de pandemia por COVID-19.

ABSTRACT The SEDIGISM (Structure, Excitation and Dynamics of the Inner Galactic Interstellar Medium) survey used the APEX telescope to map 84 deg2 of the Galactic plane between ℓ = −60° and +31° in several molecular transitions, including 13CO (2 – 1) and C18O (2 – 1), thus probing the moderately dense (∼103 cm−3) component of the interstellar medium. With an angular resolution of 30 arcsec and a typical 1σ sensitivity of 0.8–1.0 K at 0.25 km s−1 velocity resolution, it gives access to a wide range of structures, from individual star-forming clumps to giant molecular clouds and complexes. The coverage includes a good fraction of the first and fourth Galactic quadrants, allowing us to constrain the large-scale distribution of cold molecular gas in the inner Galaxy. In this paper, we provide an updated overview of the full survey and the data reduction procedures used. We also assess the quality of these data and describe the data products that are being made publicly available as part of this First Data Release (DR1). We present integrated maps and position–velocity maps of the molecular gas and use these to investigate the correlation between the molecular gas and the large-scale structural features of the Milky Way such as the spiral arms, Galactic bar and Galactic Centre. We find that approximately 60 per cent of the molecular gas is associated with the spiral arms and these appear as strong intensity peaks in the derived Galactocentric distribution. We also find strong peaks in intensity at specific longitudes that correspond to the Galactic Centre and well-known star-forming complexes, revealing that the 13CO emission is concentrated in a small number of complexes rather than evenly distributed along spiral arms.

Summary The land acquired by ICE to harness geothermal sites are subject to restoration and preservation of forest ecosystems which are suitable to generate atmospheric carbon removal. As part of the Carbon Neutral Country Program 2.0, since 2016, ICE conducts forests inventories to determine the annual rate of CO2 removal of the natural ecosystems on Alfredo Mainieri Protti Geothermal Camp (AMPGC). The total reservoir or stock of carbon accumulated in the forest biomass at the AMPGC is 348,592 tCO2-e, equivalent to 194 tCO2-e/ha ( PGF-INISEFOR, 2017 ). The base inventory conducted in 2016 identified high forests as the main carbon drain from forestall biomass. This class contributed to 71% of the stock (138 tCO2-e/ha / 193 tCO2-e/ha). Secondarily, it is the pole stands class accumulated 28% of carbon fixed by forest biomass (55 tCO2-e/ha). The other smaller fractions (necromass, leaf litter, and herbaceous vegetation) contribute with only 1% (estimated as an average of 0,34%, equivalent to 0,7 tCO2-e/ha). At the end of the fourth periodic measurement from 2016-2020, there was an average annual increase of the total mass o removals of 16.745 tCO2-e/year in a total area of 1.450 ha with forest coverage, equivalent an average estimate by ha of 11.55 tCO2-e/ha-year.

We use the 13CO(2-1) emission from the SEDIGISM high-resolution spectral-line survey of the inner Galaxy, to extract the molecular cloud population with a large dynamic range in spatial scales, using the SCIMES algorithm. This work compiles a cloud catalogue with a total of 10663 molecular clouds, 10300 of which we were able to assign distances and compute physical properties. We study some of the global properties of clouds using a science sample, consisting of 6664 well resolved sources and for which the distance estimates are reliable. In particular, we compare the scaling relations retrieved from SEDIGISM to those of other surveys, and we explore the properties of clouds with and without high-mass star formation. Our results suggest that there is no single global property of a cloud that determines its ability to form massive stars, although we find combined trends of increasing mass, size, surface density and velocity dispersion for the sub-sample of clouds with ongoing high-mass star formation. We then isolate the most extreme clouds in the SEDIGISM sample (i.e. clouds in the tails of the distributions) to look at their overall Galactic distribution, in search for hints of environmental effects. We find that, for most properties, the Galactic distribution of the most extreme clouds is only marginally different to that of the global cloud population. The Galactic distribution of the largest clouds, the turbulent clouds and the high-mass star-forming clouds are those that deviate most significantly from the global cloud population. We also find that the least dynamically active clouds (with low velocity dispersion or low virial parameter) are situated further afield, mostly in the least populated areas. However, we suspect that part of these trends may be affected by some observational biases, and thus require further follow up work in order to be confirmed.

Low protein diets supplied during the growing period of pigs can diminish their growth rate and increase the intramuscular fat (IMF) content which affects the sensorial and technological characteristics of the products. In the present study, the effects of a low protein diet supplied during the growing period of Duroc × Iberian crossbred pigs on several phenotypic traits and on liver and longissimus dorsi transcriptome were analysed at the beginning (EARLY) and at the end (LATE) of the growing period. Two experimental groups of 10 crossbred pigs each were fed two isocaloric diets with different protein content: control diet (C) with 16.5% protein and 0.8% lysine and low protein diet (LP) with 11% CP and 0.6% lysine. Animals fed LP diet have a slower growth than those fed C diet, but no effect of LP diet was observed on the IMF content. The transcriptomes of liver and longissimus dorsi were characterised and quantified through RNA-sequencing (RNA-seq). In liver, 134 and 480 differentially expressed annotated genes and new isoforms (DEGs) were detected between C and LP diets for EARLY and LATE animals, respectively. In muscle, 128 and 68 DEGs were detected at EARLY and LATE time-points. Functional interpretation revealed that LP diet may inhibit immune system molecules and processes in both tissues at EARLY stage. In liver, the DEGs mainly affect lipid and cholesterol metabolic processes, while in muscle, the expression changes would be involved in growth, development and meat quality. In conclusion, a low protein diet supplied during the growing period seems to slow down the growth of Duroc × Iberian crossbred pigs, but it also seems to affect multiple biological processes that could compromise the immune system of Duroc × Iberian crossbred pigs. Therefore, these results question the adequacy of this type of regime in Duroc × Iberian pigs that must be studied in greater depth before being implemented.

Angiotensin converting enzyme 2 (ACE2), a component of the renin-angiotensin system (RAS), has been identified as the receptor for the SARS-CoV-2. Several RAS components including ACE2 and its substrate Ang II are present in both eye and skin, two stratified squamous epithelial tissues that isolate organisms from external environment. Our recent findings in cornea and others in both skin and eye suggest contribution of this system, and specifically of ACE2 in variety of physiological and pathological responses of these organ systems. This review will focus on the role RAS system plays in both skin and cornea, and will specifically discuss our recent findings on ACE2 in corneal epithelial inflammation, as well as potential implications of ACE2 in patients with COVID-19.