AbstractIntroductionWhile seasonal influenza and other respiratory pathogens cause significant morbidity and mortality each year, the community-based burden of these infections remains incompletely understood. Understanding the prevalence, epidemiology, and transmission dynamics of respiratory pathogen infections among community-dwelling individuals is essential during pandemic and epidemic settings and for developing pandemic-preparedness infrastructure.Methods and AnalysisWe present the protocol for a novel, city-wide home-based cross-sectional study in the Seattle Metropolitan area, utilizing rapid delivery systems for self-collection of a nasal swab and return to the laboratory for respiratory pathogen testing. All participation takes place electronically, including recruitment, consent, and data collection. Within 48 hours of participants self-reporting respiratory symptoms, a nasal swab kit is delivered to the household via a courier service. Demographic and illness characteristics are collected at the time of sample collection and recovery and behavioral information collected one week later. Specimens are tested in the laboratory for multiple respiratory pathogens, and results are available on a public website for participants.Ethics and DisseminationThe study was approved by the University of Washington Institutional Review Board (Protocol #00006181). Results will be disseminated through peer-reviewed publications, talks at conferences, and on the Study Website (www.seattleflu.org).Article SummaryThe findings of this study will inform whether a home-based approach to city-wide respiratory surveillance is possible in epidemic settingsA key strength of this study is that it is conducted across diverse neighborhoods spanning a major metropolitan areaClinical findings may not be generalizable, as they are reliant on self-report (vaccination status, symptoms, healthcare utilization, etc.)

87: Theileria buffeli Infections in Grazing Cattle Located in Mountainous Areas of the Republic of Korea Kyoung-Seong Choi*, Joon-Seok Chae, Do-Hyeon Yu, Jinho Park, Bae-Keun Park, Hyeong-Chul Kim Abstract #88: Small Mammals as Hosts of Ehrlichia muris eauclairensis and Other Rickettsial Pathogens in Eau Claire, Wisconsin Bryan N. Ayres*, Audrey E. Osterbind, Jamie L. Perniciaro, Joy A. Hecht, William L. Nicholson Abstract #89: Serological Survey of Canine Tick-borne Infections Using Species-specific Serological Markers Jiayou Lui*, M. E. McCown, C. Fiorello, D. Scorpio, M. Filipovic, J. Saucier, B. Thatcher, R. Chandrashekar Abstract #90: Consistencies in Spatio-Temporal Trends, and Climatic and Other Geospatial Risk Factors for Rickettsial Diseases in the Central Midwestern United States Ram K. Raghavan*, Gary A. Anderson, Doug. G. Goodin, Roman R. Ganta88: Small Mammals as Hosts of Ehrlichia muris eauclairensis and Other Rickettsial Pathogens in Eau Claire, Wisconsin Bryan N. Ayres*, Audrey E. Osterbind, Jamie L. Perniciaro, Joy A. Hecht, William L. Nicholson Abstract #89: Serological Survey of Canine Tick-borne Infections Using Species-specific Serological Markers Jiayou Lui*, M. E. McCown, C. Fiorello, D. Scorpio, M. Filipovic, J. Saucier, B. Thatcher, R. Chandrashekar Abstract #90: Consistencies in Spatio-Temporal Trends, and Climatic and Other Geospatial Risk Factors for Rickettsial Diseases in the Central Midwestern United States Ram K. Raghavan*, Gary A. Anderson, Doug. G. Goodin, Roman R. Ganta Abstract #91: A Distributional Survey of Ticks (Acari: Ixodida) in Georgia, USA William L. Nicholson* Abstract #92: Spotted Fever Group Rickettsiae in Baltic Region Algimantas Paulauskas*, Jana Radzijevskaja, Asta Aleksandraviciene91: A Distributional Survey of Ticks (Acari: Ixodida) in Georgia, USA William L. Nicholson* Abstract #92: Spotted Fever Group Rickettsiae in Baltic Region Algimantas Paulauskas*, Jana Radzijevskaja, Asta Aleksandraviciene Abstract #93: Spotted Fever Group Rickettsia Species Identified among Ticks in Georgia Ekaterine Zhgenti*, Roena Sukhiashvili, Richard J. Obiso, Christina M. Farris, Ju Jiang, Allen L. Richards Abstract #94: Risk Assessment for Vector-borne Diseases at Gateway National Recreation Area, NY: Monitoring Ticks, Preliminary Study, 2014 Ellen Stromdahl*, Garrett Heck, Mary Vince, Waheed Bajwa, Zahir Shah, David Wong93: Spotted Fever Group Rickettsia Species Identified among Ticks in Georgia Ekaterine Zhgenti*, Roena Sukhiashvili, Richard J. Obiso, Christina M. Farris, Ju Jiang, Allen L. Richards Abstract #94: Risk Assessment for Vector-borne Diseases at Gateway National Recreation Area, NY: Monitoring Ticks, Preliminary Study, 2014 Ellen Stromdahl*, Garrett Heck, Mary Vince, Waheed Bajwa, Zahir Shah, David Wong Abstract #95: Evaluating an Ecological Niche Model for Rickettsia montanensis Infected Dermacentor variabilis Based on Ticks Collected from Humans Using Environmentally Captured Ticks Heidi K. St. John*, Penny M. Masuoka, Melissa K. Miller, Melissa L. Adams, Johanna G. Flyer-Adams, Ju Jiang, Patrick J. Rozmajzl, Ellen Y. Stromdahl, Allen L. Richards95: Evaluating an Ecological Niche Model for Rickettsia montanensis Infected Dermacentor variabilis Based on Ticks Collected from Humans Using Environmentally Captured Ticks Heidi K. St. John*, Penny M. Masuoka, Melissa K. Miller, Melissa L. Adams, Johanna G. Flyer-Adams, Ju Jiang, Patrick J. Rozmajzl, Ellen Y. Stromdahl, Allen L. Richards Abstract #96: Survey for Rickettsia Species in Dermacentor variabilis Ticks Collected from National Parks and Monuments across the Eastern United States Sandor E. Karpathy*, Joy A. Hecht, Tammi L. Johnson, Rebecca J. Eisen, Christopher D. Paddock Abstract #97: Flea-borne Rickettsiae in Southeastern Georgia, USA Marina E. Eremeeva*, Danielle Capps, Amanda Jo Williams-Newkirk, Gregory A. Dasch, Lorenza Beati, Lance Durden96: Survey for Rickettsia Species in Dermacentor variabilis Ticks Collected from National Parks and Monuments across the Eastern United States Sandor E. Karpathy*, Joy A. Hecht, Tammi L. Johnson, Rebecca J. Eisen, Christopher D. Paddock Abstract #97: Flea-borne Rickettsiae in Southeastern Georgia, USA Marina E. Eremeeva*, Danielle Capps, Amanda Jo Williams-Newkirk, Gregory A. Dasch, Lorenza Beati, Lance Durden Abstract #98: Prevalence of Q Fever and Rickettsia in Ticks in Northeastern Azerbaijan Rita Ismayilova*, Agarahim Mammadsalahov, Rakif Abdullayev, Esmiralda Seyidova, Sabina Ibrahimova, Elvin Naghiyev98: Prevalence of Q Fever and Rickettsia in Ticks in Northeastern Azerbaijan Rita Ismayilova*, Agarahim Mammadsalahov, Rakif Abdullayev, Esmiralda Seyidova, Sabina Ibrahimova, Elvin Naghiyev Abstract #99: Coxiella burnetii Small RNA 12 (CbsR12) Targets carA Transcripts In vitro Shaun Wachter*, Indu Warrier, Michael F. Minnick99: Coxiella burnetii Small RNA 12 (CbsR12) Targets carA Transcripts In vitro Shaun Wachter*, Indu Warrier, Michael F. Minnick Abstract #100: Alterations in Dermacentor andersoni Transcription in Response to Anaplasma marginale Infection Susan M. Noh*, Forgivemore Magunda, Sebastian Aguilar, Kelly A. Brayton Abstract #101: Characterization of Small, Noncoding RNAs in Rickettsia prowazekii Casey L. C. Schroeder*, Hema P. Narra, Mark Rojas, Abha Sahni, Kamil Khanipov, Jignesh Patel, Yuriy Fofanov, Sanjeev K. Sahni100: Alterations in Dermacentor andersoni Transcription in Response to Anaplasma marginale Infection Susan M. Noh*, Forgivemore Magunda, Sebastian Aguilar, Kelly A. Brayton Abstract #101: Characterization of Small, Noncoding RNAs in Rickettsia prowazekii Casey L. C. Schroeder*, Hema P. Narra, Mark Rojas, Abha Sahni, Kamil Khanipov, Jignesh Patel, Yuriy Fofanov, Sanjeev K. Sahni Abstract #102: Metagenomics of Head Lice Using the Ion Torrent PGM Gregory A. Dasch*, Zachary C. Holmes, T. Brian Shirey, Marina E. Eremeeva, Sarah Zohdy102: Metagenomics of Head Lice Using the Ion Torrent PGM Gregory A. Dasch*, Zachary C. Holmes, T. Brian Shirey, Marina E. Eremeeva, Sarah Zohdy Abstract #103: Decoding the Post-transcriptional Regulatory Networks in Rickettsia conorii Hema P. Narra*, Casey L.C. Schroeder, Abha Sahni, Yuriy Fofanov, Sanjeev K. Sahni Abstract #104: Cytoskeleton Interactions with the Coxiella burnetii Parasitophorous Vacuole Heather Miller*, Charlie Larson, Robert Heinzen103: Decoding the Post-transcriptional Regulatory Networks in Rickettsia conorii Hema P. Narra*, Casey L.C. Schroeder, Abha Sahni, Yuriy Fofanov, Sanjeev K. Sahni Abstract #104: Cytoskeleton Interactions with the Coxiella burnetii Parasitophorous Vacuole Heather Miller*, Charlie Larson, Robert Heinzen Abstract #105: Examination of Tick Vectorial Capacity Factors during Tick-borne Bacterial Infection Kathryn E. Reif*, Jessica K. Ujzco, Deb C. Alprin, Susan M. Noh Abstract #106: Nuclear Trafficking of the Anti-apoptotic Coxiella burnetii Effector Protein AnkG Requires Binding to p32 and Importin-α1 Walter Schäfer*, Rita A. Eckart, Benedikt Schmid, Kerstin Hof, Yves Muller, Bushra Amin, Anja Lührmann105: Examination of Tick Vectorial Capacity Factors during Tick-borne Bacterial Infection Kathryn E. Reif*, Jessica K. Ujzco, Deb C. Alprin, Susan M. Noh Abstract #106: Nuclear Trafficking of the Anti-apoptotic Coxiella burnetii Effector Protein AnkG Requires Binding to p32 and Importin-α1 Walter Schäfer*, Rita A. Eckart, Benedikt Schmid, Kerstin Hof, Yves Muller, Bushra Amin, Anja Lührmann Abstract #107: Effects of Novel Bacteriophage on Abalone Exposed to Candidatus Xenohaliotis californiensis – The Causative Agent of Abalone Withering Syndrome James D. Moore*, Ashley Vater, Barbara A. Byrne Abstract #108: Ehrlichia chaffeensis TRP120 Effector Autoubiquitinates and Contains a C-terminal HECT E3 Ligase Domain Bing Zhu*, Tierra R. Farris, Seema Das, Jere W. McBride Abstract #109: Ehrlichia chaffeensis TRP32 is a Nucleomodulin that Directly Regulates Host Gene Expression Tierra Farris*, Bing Zhu, Jere W. McBride107: Effects of Novel Bacteriophage on Abalone Exposed to Candidatus Xenohaliotis californiensis – The Causative Agent of Abalone Withering Syndrome James D. Moore*, Ashley Vater, Barbara A. Byrne Abstract #108: Ehrlichia chaffeensis TRP120 Effector Autoubiquitinates and Contains a C-terminal HECT E3 Ligase Domain Bing Zhu*, Tierra R. Farris, Seema Das, Jere W. McBride Abstract #109: Ehrlichia chaffeensis TRP32 is a Nucleomodulin that Directly Regulates Host Gene Expression Tierra Farris*, Bing Zhu, Jere W. McBride Abstract #110: Molecular Detection of Rickettsia Species in Ticks and Fleas in Eastern Missouri and Southern Illinois Catherine Santanello*, Redir Barwari110: Molecular Detection of Rickettsia Species in Ticks and Fleas in Eastern Missouri and Southern Illinois Catherine Santanello*, Redir Barwari Abstract #111: Molecular Survey of Arthropod Vectors for Rickettsia in the Eastern Thailand Darunee Utennam*, Kamonwan Siriwatthanakul, Chakrit Hirunpetcharat, Wuttikorn Rodkvamtook, Charnchudhi Chanyasanha, Pimmada Sakpisal, Narupon Kuttasingkee, Yutthapong Sudsawat, Chanakan Suwanbongkot, Jariyanart Gaywee Abstract #112: Rickettsia cf sp. Infection in Thailand Jariyanart Gaywe*, Wuttikon Rodkvamtook, Walairat Pronwiroon, Darunee Utainnam, Piyanate Salyakumthorn, Pimmada Sakpisal, Narupon Kuttasingkee, Kamonwan Siriwatthanakul, Chanakan Suwanbongkot, Allen L. Richards, Suradet Jaruchinda111: Molecular Survey of Arthropod Vectors for Rickettsia in the Eastern Thailand Darunee Utennam*, Kamonwan Siriwatthanakul, Chakrit Hirunpetcharat, Wuttikorn Rodkvamtook, Charnchudhi Chanyasanha, Pimmada Sakpisal, Narupon Kuttasingkee, Yutthapong Sudsawat, Chanakan Suwanbongkot, Jariyanart Gaywee Abstract #112: Rickettsia cf sp. Infection in Thailand Jariyanart Gaywe*, Wuttikon Rodkvamtook, Walairat Pronwiroon, Darunee Utainnam, Piyanate Salyakumthorn, Pimmada Sakpisal, Narupon Kuttasingkee, Kamonwan Siriwatthanakul, Chanakan Suwanbongkot, Allen L. Richards, Suradet Jaruchinda Abstr