Journal of Immunotherapy with Emphasis on Tumor Immunology: November 1993 - Volume 14 - Issue 4 - p 365

Murine Interleukin 2 (IL2) was denatured with sodium dodecyl sulfate (SDS) with or without concomitant reduction of disulfide bonds. Between 50 and 100% of the activity was recovered upon removal of SDS. When SDS-denatured IL2 was chromatographed on a calibrated gel filtration column in the presence of SDS, it eluted with proteins of m.w. 16,000. This value is supported by sedimentation velocity studies in SDS-containing glycerol gradients. Three activities previously associated with IL2, namely the obligatory role in thymocyte mitogenesis, helper activity in the generation of cytotoxic T lymphocytes, and T cell growth factor activity, co-purified after SDS denaturation. These results indicate that the essential component of murine IL2 is a peptide of m.w. about 16,000. The 3 species of Interleukin 2 studied so far--rat, human, and murine--thus can all exist as polypeptide chains of 15,000 to 16,000 m.w. The murine factor is normally isolated as a larger entity, of about twice this m.w.

Immunological ReviewsVolume 51, Issue 1 p. 157-175 Cellular Origins and Targets of Costimulator (IL2) Verner Paetkau, Verner Paetkau Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, CanadaSearch for more papers by this authorJennifer Shaw, Jennifer Shaw Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, CanadaSearch for more papers by this authorGordon Mills, Gordon Mills Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, CanadaSearch for more papers by this authorBarry Caplan, Barry Caplan Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, CanadaSearch for more papers by this author Verner Paetkau, Verner Paetkau Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, CanadaSearch for more papers by this authorJennifer Shaw, Jennifer Shaw Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, CanadaSearch for more papers by this authorGordon Mills, Gordon Mills Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, CanadaSearch for more papers by this authorBarry Caplan, Barry Caplan Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, CanadaSearch for more papers by this author First published: August 1980 https://doi.org/10.1111/j.1600-065X.1980.tb00320.xCitations: 36AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat REFERENCES Aarden, L. A. et al. (1979) Revised nomenclature for antigen-nonspecific T cell proliferation and helper factors. J. Immunol. 123, 2928–2929. Bevan, M. J. & Cohn, M. (1975) Cytotoxic effects of antigen and mitogen-induced T cells on various targets. J. Immunol. 114, 559–565. Cantor, H. & Boyse, E. A. (1977) Lymphocytes as models for the study of mammalian cellular differentiation. Immunol. Rev. 33, 105–124. Chen, D.-M. & DiSabato, G. (1976) Further studies on the thymocyte stimulating factor. Cell. Immunol. 22, 211–224. Delovitch, T. L. & McDevitt, H. O. (1977) In vitro analysis of allogeneic lymphocyte interaction. I. Characterization and cellular origin of an la-positive helper factor- allogeneic effect factor. J. exp. Med. 146, 1019. DiSabato, G., Chen, D.-M. & Erickson, J. W. (1975) Production by murine spleen cells of an activity stimulating the PHA-responsiveness of thymus lymphocytes. 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The lymphokine Interleukin 2(IL2) restores T cell responses in a number of in vitro systems where immunogenicity has been compromised. UV irradiation of the stimulating allogeneic cells in a mixed leukocyte culture eliminates the production of cytotoxic T lymphocytes and greatly reduces the DNA synthesis response. IL2 restores both parameters. UV-irradiated stimulators are also unable to induce the normal production of IL2 which is observed in a mixed leukocyte culture. The cytotoxic activity of allogeneically stimulated thymocytes is almost completely lost within 24 hours after removal of IL2 at 5 days, indicating that the lymphokine is continuously required to maintain CTL. Thymocytes in 4-day cultures do not adsorb IL2 unless they are simultaneously activated with a mitogen. Finally, IL2 does not adequately restore a secondary response to the purified protein derivative of tuberculin (PPD) in adherent-cell-depleted cultures, indicating that macrophages, in addition to being required for IL2 production, have other functions. These probably include the presentation of soluble antigens to responding cells.