An investigation was conducted during the year 2016-2017 with 20 germplasm accessions of Okra (Abelmoschus esculentus (L.) Moench) were evaluated in a randomized block design with two replications at Vegetable Research Farm, The field experiment was carried out at Horticulture Research Scheme (Vegetable) and Department of Horticulture, College of Agriculture, VNMKV, Parbhani, during the Kharif season,2016-17 to identify the diverse genetic stocks for the use in hybridization programme through genetic diversity based on Mahalanobis D2 statistics. The data was recorded on eighteen yield contributing characters on accessions raised in Randomized Block Design in two replications. The germplasm accessions were grouped into five different clusters. The maximum inter-cluster distance was found between clusters IV and V (D=281.31) followed by clusters I and V (D=253.84), clusters I and III (D=228.56), cluster III and IV (D=228.16) and clusters II and IV (D=180.62) indicated that these groups of genotypes were highly divergent from each other. Among the five clusters, the Cluster I recorded high mean performance for five characters viz., Plant height, Number of branches per plant, Fruit weight, Iron content, Fruit yield per plant,. Regarding the grouping of clusters, cluster I has maximum number of entries i.e., 10 genotypes followed by Cluster II with 6 genotypes, Cluster IV with 2 genotypes and Cluster III and Cluster V with one each respectively. The D2 analysis for eighteen characters partitioned the twenty genotypes into five clusters. The maximum genetic divergence was observed between cluster III and V followed by between clusters I and V. The maximum intra cluster distance was shown by cluster II. The characters number of branches per plant, number of fruits per plant, total number of pickings, fruit weight, fruit length and fruit yield per plant contributed greatly towards diversity. The clusters showing high genetic divergence could be effectively utilized in heterosis breeding programme. If a breeding programme is used at improving growth attributes like plant height, then cluster III showing maximum plant height can be utilized in breeding programme. Therefore, a plant breeder may keep in mind the above aspects to obtain superior hybrids and good recombinants.