Three chromatographically distinct tRNAs fMet from E. coli K-12 MO were separated by reversed-phase chromatography and designated tRNA A fMet , tRNA B fMet , and tRNA 3 fMet . The tRNA A fMet corresponds to the published sequence for tRNA fMet ( E. coli ). The tRNA B fMet differs from tRNA A fMet in that the 4-thiouridine in nucleotide position 8 has interacted with cytidine in position 13 to form a cross-linked product. The tRNA 3 fMet differs from tRNA A fMet in that 7-methyl-guanosine (in position 47) has been replaced by adenosine.

Reversed-phase chromatography has been applied to the rapid separation of E. coli 5S rRNA and has also been adapted for use on an analytical scale for the rapid (about 30 min) separation of small quantities (0.1 Ap260 unit) of l6s and 23S rRNAs. Compared to other techniques, this nondestructive method is faster, more sensitive, and gives better resolution.

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSeparation of Escherichia coli ribosomal ribonucleic acids by reversed-phase chromatographyB. Zane Egan, J. E. Caton, and A. D. KelmersCite this: Biochemistry 1971, 10, 10, 1890–1894Publication Date (Print):May 11, 1971Publication History Published online1 May 2002Published inissue 11 May 1971https://doi.org/10.1021/bi00786a025RIGHTS & PERMISSIONSArticle Views34Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (529 KB) Get e-Alertsclose Get e-Alerts