The exoskeleton of crabs serves functions in protection, support, and sensing. Among the microstructures that compose the exoskeleton, the Bouligand structure is known to contribute to its mechanical properties. Previous research on the influence of microstructures on the mechanical properties of the crustacean exoskeleton has primarily focused on stacking height (SH), yet it remained controversial whether SH is the dominant determining factor of the mechanical properties. In this study, we comprehensively analyzed the pitch angle, diameter of the chitin-protein fiber, and the interlamellar spacing in the Bouligand structure to compare their contribution to the mechanical properties. We found that in vent crabs, the carapace was harder than the claw, while the opposite was observed in ghost crabs. In vent crabs, SH was 1.95 times greater than in the claw, a difference likely attributable to the pitch angle-the only microstructural feature that varied. In contrast, no structural differences were detected between regions in ghost crabs, where SH was extremely small (< 1 μm) and thus mechanical properties appear to be governed by material characteristics rather than structure. These findings indicate that pitch angle influences the mechanical properties of the crab exoskeleton only when SH is sufficiently large.

Plastic contamination is a global pervasive issue, extending from coastal areas and open oceans to polar regions and even the deep sea. Microplastic (MP) contamination in hydrothermal vents, which are known for their high biodiversity even under extreme conditions, has remained largely unexplored. Here, we present, for the first time, MP pollution in a deep-sea hydrothermal vent at one of the biodiversity hotspots & horbar;the Central Indian Ridge. Not only the environment (seawater: 2.08 +/- 1.04 MPs/L, surface sediments: 0.57 +/- 0.19 MP/g) but also all six major benthic species investigated were polluted by MPs. MPs mainly consisted of polypropylene, polyethylene terephthalate, and polystyrene fragments <= 100 mu m and were characterized as being either transparent or white in color. Remarkably, bioaccumulation and even biomagnification of microplastics were observed in the top predators of the ecosystem, such as squat lobsters (14.25 +/- 4.65 MPs/individual) and vent crabs (14.00 +/- 2.16 MPs/individual), since they contained more MPs than animals at lower trophic levels (e.g., mussels and snails, 1.75-6.00 average MPs/individuals). These findings reveal MP contamination of an ecosystem in a hydrothermal vent, thereby suggesting that their accumulation and magnification can occur in top-level animals, even within remote and extreme environments.

Limitations on energetic resources create evolutionary trade-offs, prompting us to investigate if investment in claw strength remains consistent across crustaceans living in diverse habitats. Decapod crustaceans living in deep-sea hydrothermal vents are ideal for this study due to their extreme environment. In this study, we investigated whether decapods (blind crab Austinograea sp. and the squat lobster Munidopsis lauensis) living in deep-sea hydrothermal vents prioritize investing in strong claws compared to the carapace, like coastal decapods. We analyzed exoskeleton morphology, mechanical properties, structures, and elemental composition in both the carapace and claws of four Decapoda species (two each from Brachyura and Anomura infraorders) in vent and coastal habitats. Coastal decapods had ∼4-9 times more teeth on their claw cutting edge than the vent species. Further, only the coastal species exhibited higher firmness in their claws than in their carapaces. Each infraorder controlled exoskeletal hardness differently: Brachyura changed the stacking height of the Bouligand structure, while Anomura regulated magnesium content in the exoskeleton. The vent decapods may prioritize strengthening their carapace over developing robust claws, allocating resources to adapt to the harsh conditions of deep-sea hydrothermal vents. This choice might enhance their survival in the extreme environment, where carapace strength is crucial for protecting internal organs from environmental factors, rather than relying on the powerful claws seen in coastal decapods for a competitive advantage.

Continuous emissions of anthropogenic CO2 are changing the atmospheric and oceanic environment. Although some species may have compensatory mechanisms to acclimatize or adapt to the changing environment, most marine organisms are negatively influenced by climate change. In this study, we aimed to understand the compensatory mechanisms of the Antarctic clam, Laternula elliptica, to climate-related stressors by using archived shells from 1995 to 2018. Principal component analysis revealed that seawater pCO2 and salinity in the Antarctic Ocean, which have increased since the 2000's, are the most influential factors on the characteristics of the shell. The periostracum thickness ratio and nitrogen on the outermost surface have increased, and the dissolution area (%) has decreased. Furthermore, the calcium content and mechanical properties of the shells have not changed. The results suggest that L. elliptica retains the mechanism of protecting the shell from high pCO2 by thickening the periostracum as a phenotype plasticity.

Animals living in extreme environments, such as hydrothermal vents, would be expected to have evolved protective shells or exoskeletons to maintain homeostasis. The outer part of the exoskeleton of vent crabs ( Austinograea sp.) in the Indian Ocean hydrothermal vent was one of the hardest (approximately 7 GPa) biological materials ever reported. To explore the exoskeletal characteristics of vent crabs which enable them to adapt to severe environments, a comparative analysis was conducted with the Asian paddle crab ( Charybdis japonica ) living in coastal areas. Nanoindentation, thermogravimetric analysis, scanning electron microscopy, energy dispersive x-ray analysis, and Raman spectroscopy were used to analyze the mechanical properties, thermal stability, structure, surface components, and the composition of compounds, respectively. Though both species have four-layered exoskeletons, the outermost layer of the vent crab, a nano-granular structure, was much thicker than that of the coastal crab. The proportions of aluminum and sulfur that constitute the epicuticle of the exoskeleton were higher in the vent crab than in the coastal crab. There was a lack of water or volatile substances, lots of CaCO 3 , and no carotenoid-based compounds in the exoskeleton of the vent crab. These might have improved the mechanical properties and thermal stability of the hydrothermal species.

The Antarctic limpet, Nacella concinna, is one of the most abundant benthic marine invertebrates found in the intertidal zone of King George Island, Antarctica. The shell of N. concinna is often encrusted with the coralline algae Clathromorphum obtectulum. In this study, to reveal the relationship between the limpet and coralline algae, we examined how the coralline algae affect the physical condition (survival and health) and morphology of the limpet. We cultured the limpets for 22 days and compared mortality, weight, condition factor (CF), fatty acid content, and the structure of the shell surface between limpets both with and without coralline algae in the laboratory. We also measured the environmental factors (i.e., temperature, pH, and salinity) of the seawater at each sampling site and the CF of the limpets and correlated them with coverage of coralline algae. The presence of coralline algae significantly increased the mortality of the limpets by 40% and the shell weight by 1.4-fold but did not affect the CF. Additionally, coralline algae altered the fatty acid profiles related to the limpet’s lipid metabolism (saturated fatty acids (SFA) and some polyunsaturated fatty acids (PUFA)). Specifically, C16:0, C17:0, C18:0, and total SFA increased, whereas C18:2 and C18:3 decreased. However, observations with a scanning electron microscope showed that shell damage in limpets with coralline algae was much less than in limpets without coralline algae, suggesting that coralline algae may provide protection against endolithic algae. The area of coralline algae on the limpet shell was positively correlated with the pH and temperature of the seawater. The results suggest that although coralline algae are generally assumed to be parasitical, the relationship between N. concinna and coralline algae may change to mutualism under certain conditions.

Ghost fishing via a derelict fishing gear (DFG) is a critical threat to marine organisms. To explore the effect of DFG on sea turtle strandings, the DFG distribution was compared at two sites on Jeju Island (South Korea) with a contrasting number of strandings. Coastal areas in northern Jeju Island were surveyed during dives with scuba equipment, and the DFG from two sites, Gwideok-ri and Sinchang-ri was collected and compared in terms of quantity and size of the items. Fishing line was more common, longer, and thicker in Gwideok-ri than in Sinchang-ri, while other types of DFG did not differ between the two sites. In addition, necropsies on two loggerhead sea turtles discovered on Jeju Island found fishing lines with fishing hooks in the oral cavity of both carcasses. This suggests that derelict recreational fishing lines may pose a significant threat to sea turtles in coastal areas.

Hydrothermal vents are chemosynthetically driven ecosystems and one of the most extreme environments on Earth. Vent communities exhibit remarkable taxonomic novelty at the species and supra-species levels, and over 80% of vent species are endemic. Here, we used mitochondrial DNA to identify the biogeographic distribution of Munidopsis lauensis and the heme-binding regions of A1-type COX1 from six species (including M. lauensis) to investigate whether genetic variation in the protein structure affects oxygen-binding ability. We verified the identity of Indian Ocean specimens by comparing sequences from the barcoding gene mitochondrial cytochrome oxidase subunit 1 (COI) with known M. lauensis sequences from the NCBI database. The data show that these are the first recorded specimens of M. lauensis in the Indian Ocean; previously, this species had been reported only in the southwest Pacific. Our findings support the hypothesis that vent fauna in the Pacific and Indian Oceans can interact via active ridges. In the case of the mitochondrial DNA-binding site, the arrangement of heme-binding ligands and type A1 motif of M. lauensis was identical to that in other species. Moreover, our findings suggest that the mechanism of oxygen binding is well conserved among species from terrestrial organisms to hydrothermal extremophiles. Overall, dispersal of the same species to geologically separated hydrothermal vents and conserved heme-binding regions in mitochondrial proteins suggest that hydrothermal species might have evolved from shallow sea organisms and became distributed geographically using a dispersion corridor.

We investigated the response of the Manila clam Venerupis philippinarum to possible temperature and salinity changes in a holding facility. First, clams were exposed to four temperatures for 15 days. Valve closure and survival of clams exposed to seawater at 18 °C were higher than that of those exposed to seawater at 24 °C. Second, clams were exposed to six salinities for 15 days. Survival of clams exposed to two salinity fluctuation conditions (24–30 and 27–24 psu) was lower than that of clams exposed to constant 30 psu conditions. Valve closures of clams exposed to constant low salinity conditions (24 psu) and two salinity fluctuation conditions (24–30 and 27–24 psu) were higher than those exposed to constant 30 psu conditions. Lastly, clams were exposed to two different temperatures and three different salinity conditions for 8 days. Valve closure and survival decreased significantly under the combination of 24 °C and 18 psu. These results suggest that an increase in temperature or a wider range of salinity fluctuations are detrimental to the survival of the Manila clam. The synergistic effect of temperature and salinity stressors may decrease the survival period of clams compared to the effect of a single stressor.

The Indian Ocean hydrothermal vent is a region where a new oceanic crust is formed by magma at the interface of the deep-sea bed over 2000 m in depth. Here we examined for the first time the exoskeleton structure and mechanical properties of the bythograeid crab Austinograea Rodriguezensis living in hydrothermal vents. Scanning electron microscope and energy dispersive x-ray were used for structural analysis, and a nanoindentation system was used for mechanical analysis. The exoskeleton was divided into four layers: epicuticle, exocuticle, endocuticle, and membrane. The thickness of each layer was different from that of other crustaceans previously reported. Additionally, the number of constituent elements, composition ratio, and hardness of each layer were unique among previously studied crabs. This observation indicates that those characteristics might have evolved for creatures with a hard exoskeleton living in the deep-sea hydrothermal vent.