Objective This study aimed to identify differentially expressed (DE) mRNAs and long noncoding RNAs (lncRNAs) between oral lichen planus (OLP) and skin lichen planus (SLP) to elucidate the molecular mechanisms driving the higher malignant transformation risk associated with OLP. Design Tissue samples were collected from SLP and OLP patients who were diagnosed at between 2008 and 2012. RNA sequencing was conducted to analyze the differentially expressed (DE) mRNAs and lncRNAs in OLP compared to SLP. We performed Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Set Enrichment Analysis (GSEA), and Protein-Protein Interaction (PPI) Network analyses using the identified DE mRNAs in OLP. Results Analysis revealed 1,106 OLP-specific DE mRNAs (555 upregulated) and 348 DE lncRNAs (201 upregulated). KEGG and GSEA highlighted significant enrichment in IL-17 and TNF-α/NF-κB signaling pathways. PPI network analysis identified key hub genes (CXCL1, CXCL2, CXCL8, JUN, IL1B, IL6) linked to IL-17 signaling. Notable dysregulation included upregulated oncogenic lncRNAs (H19, MIR31HG, SNHG15) and downregulated tumor suppressors (CA3-AS1, HAND2-AS1). Furthermore, 1,228 co-expressed lncRNA-mRNA pairs were identified through trans-target prediction. Conclusion The identified DE mRNAs enriched in IL-17 and TNF-α/NF-κB signaling pathways, along with the dysregulation of oncogenic and tumor suppressor lncRNAs, provide molecular evidence for the higher risk of malignant transformation associated with OLP compared to SLP. These DE mRNAs and lncRNAs, along with their target genes, represent potential novel biomarkers or therapeutic targets for the diagnosis and management of OLP.

Triptolide, the major component of Chinese herbal medicine Tripterygium wilfordii Hook F, possesses potent anticancer and anti-inflammatory effects. IL-8, a proinflammatory cytokine, is associated with cancer cell proliferation and angiogenesis. Here, we found that Triptolide has an inhibitory effect on IL-1β-induced IL-8 expression in human gastric cancer cells, via the suppression of reactive oxygen species (ROS) production, AP-1, and NF-κB activation, which in turn affects human endothelial cell angiogenetic activity in tumor microenvironments. Human gastric AGS cells were treated with IL-1β (10 ng/mL) and Triptolide (0–20 nM), and the ROS generation, ERK, AP-1, and NF-κB signaling were all investigated. These results demonstrate that Triptolide inhibits the IL-1β-induced IL-8 expression in gastric cancer cells by inhibiting ROS production and angiogenesis, via the dose-dependent attenuation of ERK, AP-1, and NF-κB activation. In this study, we showed that Triptolid inhibits ROS/ERK-mediated AP-1 and ROS-mediated NF-κB axes potentially leading to an improved treatment outcome for gastric cancer and its associated tumor microenvironment.

Obesity is a critical global health challenge linked to cardiovascular disease, type 2 diabetes, metabolic syndrome, and cancers. Current therapies often lack sustained efficacy, prompting interest in molecular strategies targeting adipogenesis. Exosomes, cell-derived extracellular vesicles, exhibit therapeutic potential due to high biocompatibility and ability to mediate intercellular communication. This study explored the anti-adipogenic effects of exosomes derived from human dental pulp stem cells (hDPSC-Exos). hDPSC-Exos were isolated from conditioned media and characterized by TEM, nanoparticle tracking analysis, and Western blotting. Their anti-adipogenic effects were evaluated in vitro using 3T3-L1 and OP9 preadipocytes via Oil Red O staining, RT-qPCR, western blotting and multi-omics analyses (RNA-seq, proteomics). Therapeutic efficacy was further validated in a diet-induced obesity mouse model. Treatment with hDPSC-Exos markedly suppressed adipocyte differentiation in vitro, reducing lipid accumulation and downregulating key adipogenic transcription factors and their downstream targets. Mechanistically, Wnt/β-catenin signaling activation mediated this inhibition. In vivo, hDPSC-Exos administration reduced body weight, fat mass, and adipogenic gene expression in obese mice. These findings reveal a novel regulatory function of hDPSC-Exos in adipogenesis, highlighting their potential as a cell-free therapy for obesity and related metabolic disorders.

Alzheimer's disease is a major neurodegenerative disorder that leads to dementia, yet specific treatments remain elusive. Although Albizzia julibrissin has been used in traditional oriental medicine to treat insomnia and disorientation by its anti-inflammatory properties, there are currently no studies in animal models. This study aims to identify potential therapeutic candidates for Alzheimer's disease by examining how 3',4',7-trihydroxyflavone (THF), isolated from Albizzia julibrissin stem bark, as a potential therapeutic candidate for Alzheimer's disease by examining memory recovery in a scopolamine-induced memory deficit mouse model. THF administration both orally and centrally in scopolamine-induced AD mice led to significant improvements in cognitive performance. Biochemical assays revealed restoration of cholinergic markers (ACh, AChE, ChAT) and an increase in BDNF levels in the hippocampus. Electrophysiological recordings confirmed that THF restored LTP reduced by scopolamine, indicating improved synaptic plasticity. These findings suggest that THF has the potential neuropharmacological agent to protect the brain from memory loss induced by Alzheimer's disease through enhancing cholinergic system activity and activating the CREB-BDNF signaling pathway in the hippocampus.

Gastric cancer (GC) is the fifth most common cancer worldwide and makes up a significant component of the global cancer burden. Helicobacter pylori (H. pylori) is the most influential risk factor for GC, with the International Agency for Research on Cancer classifying it as a Class I carcinogen for GC. H. pylori has been shown to persist in stomach acid for decades, causing damage to the stomach's mucosal lining, altering gastric hormone release patterns, and potentially altering gastric function. Epidemiological studies have shown that eliminating H. pylori reduces metachronous cancer. Evidence shows that various molecular alterations are present in gastric cancer and precancerous lesions associated with an H. pylori infection. However, although H. pylori can cause oxidative stress-induced gastric cancer, with antioxidants potentially being a treatment for GC, the exact mechanism underlying GC etiology is not fully understood. This review provides an overview of recent research exploring the pathophysiology of H. pylori-induced oxidative stress that can cause cancer and the antioxidant supplements that can reduce or even eliminate GC occurrence.

Microglia, similar to peripheral macrophages, are the primary immune cells of the central nervous system (CNS). Microglia exist in the resting state in the healthy CNS, but can be activated and polarized into either M1 or M2 subtypes for immune defense and the maintenance of CNS homeostasis by multiple stimuli. Several long noncoding RNAs (lncRNAs) mediate human inflammatory diseases and neuropathologies by regulating their target genes. However, the function of common lncRNAs that contribute to microglial activation remains unclear. Thus, we used bioinformatic approaches to identify common lncRNAs involved in microglial activation in vitro. Our study identified several lncRNAs as common regulators of microglial activation. We identified 283 common mRNAs and 53 common lncRNAs during mouse M1 microglial activation processes, whereas 26 common mRNAs and five common lncRNAs were identified during mouse M2 microglial activation processes. A total of 648 common mRNAs and 274 common lncRNAs were identified during the activation of human M1 microglia. In addition, we identified 1,920 common co-expressed pairs in mouse M1 activation processes and 25 common co-expressed pairs in mouse M2 activation processes. Our study provides a comprehensive understanding of common lncRNA expression profiles in microglial activation processes in vitro. The list of common lncRNAs identified in this study provides novel evidence and clues regarding the molecular mechanisms underlying microglial activation.

This study examined the effect of the experience of traditional food culture on value co-creation attitude. The analysis with the Q methodology of the value co-creation attitude into 17 items of 4 sub-concepts: interaction attitude, knowledge-sharing attitude, responsive attitude, and public attitude. This study was significant in that, the participants continue to seek new and special experiences; therefore, it was necessary to expand and offer unique traditional food culture experiences, and the public and private sectors should actively seek to develop and expand such experiences. And through the analysis above, it discussed ways to invigorate and diversify traditional food culture experience and the measures to hand down and inherit the traditional food culture and suggests improvements in traditional food culture experience.

Flexible intramedullary nailing is gaining popularity as an effective method of treating long-bone fractures in children.We retrospectively reviewed the records and radiographs of 56 unstable fractures of the tibia in 54 children treated between March 1997 and May 2005. All were followed up for at least two months after the removal of the nails.Of the 56 tibial fractures, 13 were open. There were no nonunions. The mean time to clinical and radiological union was ten weeks. Complications included residual angulation of the tibia, leg-length discrepancy, deep infection and failures of fixation. All achieved an excellent functional outcome.We conclude that flexible intramedullary fixation is an easy and effective method of management of both open and closed unstable fractures of the tibia in children.

Neurons in the hypothalamic arcuate nucleus relay and translate important cues from the periphery into the central nervous system. However, the gene regulatory program directing their development remains poorly understood. Here, we report that the LIM-homeodomain transcription factor Isl1 is expressed in several subpopulations of developing arcuate neurons and plays crucial roles in their fate specification. Mice with conditional deletion of the Isl1 gene in developing hypothalamus display severe deficits in both feeding and linear growth. Consistent with these results, their arcuate nucleus fails to express key fate markers of Isl1-expressing neurons that regulate feeding and growth. These include the orexigenic neuropeptides AgRP and NPY for specifying AgRP-neurons, the anorexigenic neuropeptide αMSH for POMC-neurons, and two growth-stimulatory peptides, growth hormone-releasing hormone (GHRH) for GHRH-neurons and somatostatin (Sst) for Sst-neurons. Finally, we show that Isl1 directly enhances the expression of AgRP by cooperating with the key orexigenic transcription factors glucocorticoid receptor and brain-specific homeobox factor. Our results identify Isl1 as a crucial transcription factor that plays essential roles in the gene regulatory program directing development of multiple arcuate neuronal subpopulations.

LIM homeodomain factors regulate the development of many cell types. However, transcriptional coactivators that mediate their developmental function remain poorly defined. To address these, we examined how two related NLI-dependent LIM complexes, which govern the development of spinal motor neurons and V2a interneurons, activate the transcription in the embryonic spinal cord. We found that single-stranded DNA-binding proteins are recruited to these LIM complexes via NLI, and enhance their transcriptional activation potential. Ssdp1 and Ssdp2 (Ssdp1/2) are highly expressed in the neural tube and promote motor neuron differentiation in the embryonic spinal cord and P19 stem cells. Inhibition of Ssdp1/2 activity in mouse and chick embryos suppresses the generation of motor neurons and V2a interneurons. Furthermore, Ssdp1/2 recruit histone-modifying enzymes to the motor neuron-specifying LIM complex and trigger acetylation and lysine 4 trimethylation of histone H3, which are well-established chromatin marks for active transcription. Our results suggest that Ssdp1/2 function as crucial transcriptional coactivators for LIM complexes to specify spinal neuronal identities during development.

사지마비를 보이는 6년령 시쮸 숫컷이 내원하였다. 신경검사소견 및 영상진단소견에서 경추 4번과 5번 사이, 경추 5번과 6번 사이 및 경추 6번과 7번 사이의 디스크가 탈출되어 척수를 압박하고 있는 것으로 나타났다. 오존가스를 경추 4번과 5번 사이 디스크 및 경추 6번과 7번 사이 디스크에 주입하고 경추 5번과 6번 사이는 복측 감압술로 탈출된 디스크를 제거하였다. 환축은 수술후 목 통증이 사라지고 수술후 3주경 정상보행을 나타냈다. 수술후 2개월째 자기공명영상에서 경추 5번과 6번 사이 디스크와 경추 6번과 7번 사이 디스크의 탈출된 소견이 사라졌고 경추 4번과 5번 사이의 디스크는 탈출된 정도가 현저히 줄어든 소견을 보였다. 【A 6-year-old castrated male Shih-tzu was presented because of a 1-month history of progressive tetraplegia. On the basis of the findings of neurological examination, radiographs and magnetic resonance imaging, herniated intervertebral disc of C4-C5, C5-C6 and C6-C7 compressed spinal cord. The oxygen-ozone was intra-operatively injected into the C4-C5 and C6-C7 intervertebral disc and a ventral decompression performed at C5-C6 intervertebral space. Immediately after the surgery, neck pain was disappeared and three weeks after surgery, there was complete resolution of the neurological deficits with normal gaits. On magnetic resonance images at 2-month after surgery, herniated disc materials of C5-C6 and C6-C7 were completely removed and that of C4-C5 remarkably reduced. Consequently decompression which was performed at all of the three lesions resulted in good clinical outcome without additional internal fixation for minimizing postoperative instability.】