In La Réunion, pineapple is the main fruit crop, but it is threatened by Pineapple Mealybug Wilt-associated Viruses, which are vectored by Dysmicoccus brevipes (Cockerell, 1893). In a context where control methods are drastically limited, new management strategies must be examined, including the use of local natural enemies. In this study, we evaluated the potential of three predator species present in La Réunion and known to prey on mealybugs as biological control agents of D. brevipes: the ladybirds Exochomus laeviusculus Weise, 1909 (Coleoptera: Coccinellidae) and Nephus apolonia Magro Almeida, 2020 (Coleoptera: Coccinellidae) and the brown lacewing Psectra iniqua (Hagen, 1859) (Neuroptera: Hemerobiidae). Stage-specific predation assays were conducted under laboratory conditions using four developmental stages of D. brevipes, and predator development was monitored over a 30-day period. Among the three species tested, E. laeviusculus exhibited the highest predation rates and was the only predator able to complete its development on D. brevipes. Predation was significantly higher on first- and second-instar nymphs than on older nymphs and adults, indicating a preference for early developmental stages. Conversely, N. apolonia and P. iniqua failed to complete development on D. brevipes, with predation restricted to adults feeding on early developmental stages of the pineapple mealybug.

The genus Sciomesa Tams & Bowden (Lepidoptera: Noctuidae: Noctuinae: Apameini: Sesamiina) is a predominantly Afrotropical group of stemborer moths whose convoluted taxonomic history needs to be clarified. In this study, a comprehensive examination of specimens in collections was carried out in a complementary manner with molecular analyses, resulting in the following changes: (i) 12 new Sciomesa species are described: S. lalokeli n. sp. and S. metiminko n. sp. from Ethiopia; S. elgonensis n. sp., S. nigra n. sp. and S. simillima n. sp. from Kenya; S. umvoti n. sp. from South Africa; S. iboya n. sp., S. lilomwi n. sp., S. makete n. sp., S. njombe n. sp. and S. tanganyika n. sp. from Tanzania; S. kalinzu n. sp. from Uganda; (ii) four species are reinstated as valid species: three of them, S. constantini Laporte stat. rev., S. etchecopari Laporte stat. rev. and S. mirifica Laporte stat. rev., were previously synonymized with S. mesophaea (Hampson) while S. congitae (Laporte) stat. rev. was previously synonymized with S. boulardi (Laporte); (iii) two species are synonymized: S. minuscula (Laporte) n. syn., which was previously synonymized with S. boulardi, is a synonym of S. congitae, and S. franciscae Laporte n. syn. is a synonym of S. mesophaea; (iv) one species, Sesamia punctipennis Kr & uuml;ger stat. rev., which was transferred to the Sciomesa genus, is removed from the genus Sciomesa and temporarily reinstated to the genus Sesamia Guen & eacute;e pending a new combination. A supplemental description for 12 previously described species is also provided: S. boulardi, S. bua Moyal et al., S. constantini, S. etchecopari, S. excelsa (Laporte), S. gnosia Moyal et al., S. mesophaea, S. congitae, S. mirifica, S. renibifida Berio, S. scotochroa (Hampson) and S. venata Fletcher. Host-plant records are provided for 10 Sciomesa species, confirming a pattern of phylogenetic niche conservatism on Poales plants in the Cyperaceae family. Phylogenetic and molecular species delimitation analyses were carried out on a multimarker (four mitochondrial and two nuclear genes) molecular dataset encompassing 63 Sciomesa specimens, 24 species belonging to other genera of Sesamiina, and one representative of the sister subtribe Apameina. This sampling also includes the three Malagasy species (S. betschi Viette, S. janthina Viette and S. oberthueri Viette) whose status is debated. The resulting phylogenetic framework provides the most complete picture of the evolutionary relationships of the genus Sciomesa, and results of topological tests provide further support for removing S. betschi and S. janthina from the genus Sciomesa pending new combinations. The results of molecular species delimitation analyses also overwhelmingly support the species status of the Sciomesa species sampled, even for S. mesophaea, a species with a very wide range in the Afrotropics. These analyses also reveal the existence of up to three potential new species, whose status cannot be ascertained because the sequenced individuals were either extracted at a larval stage or correspond to specimens whose genitalia were not studied when their legs were collected. R & eacute;vision du genre Sciomesa (Lepidoptera : Noctuidae : Apameini : Sesamiina) : taxonomie, phylog & eacute;nie et & eacute;cologie, avec la description de 12 nouvelles esp & egrave;ces. Le genre Sciomesa Tams & Bowden (Lepidoptera : Noctuidae : Noctuinae : Apameini : Sesamiina) est un groupe principalement afrotropical de papillons de nuit foreurs de tige, dont l'histoire taxonomique complexe doit & ecirc;tre clarifi & eacute;e. Dans cette & eacute;tude, l'examen d & eacute;taill & eacute; de nombreux sp & eacute;cimens de collections a & eacute;t & eacute; effectu & eacute; de fa & ccedil;on compl & eacute;mentaire & agrave; des analyses mol & eacute;culaires, ce qui a conduit aux changements suivants : (i) 12 nouvelles esp & egrave;ces de Sciomesa sont d & eacute;crites : S. lalokeli n. sp. et S. metiminko n. sp. d'& Eacute;thiopie ; S. elgonensis n. sp., S. nigra n. sp. et S. simillima n. sp. du Kenya ; S. kalinzu n. sp. d'Ouganda ; S. umvoti n. sp. d'Afrique du Sud ; S. iboya n. sp., S. lilomwi n. sp., S. makete n. sp., S. njombe n. sp. et S. tanganyika n. sp. de Tanzanie; (ii) quatre esp & egrave;ces sont r & eacute;tablies comme esp & egrave;ces valides : trois d'entre elles, S. constantini Laporte stat. rev., S. etchecopari Laporte stat. rev. et S. mirifica Laporte stat. rev. & eacute;taient pr & eacute;c & eacute;demment synonymes de S. mesophaea (Hampson) tandis que S. congitae (Laporte) stat. rev. & eacute;tait pr & eacute;c & eacute;demment synonyme de S. boulardi (Laporte); (iii) deux esp & egrave;ces sont mises en synonymie : Sciomesa minuscula n. syn., qui & eacute;tait pr & eacute;c & eacute;demment synonyme de S. boulardi, est un synonyme de S. congitae, et S. franciscae Laporte n. syn. est un synonyme de S. mesophaea ; (iv) une esp & egrave;ce, Sesamia punctipennis Kr & uuml;ger stat. rev., qui avait & eacute;t & eacute; transf & eacute;r & eacute;e dans le genre Sciomesa, en est retir & eacute;e et temporairement r & eacute;int & eacute;gr & eacute;e dans le genre Sesamia Guen & eacute;e en attendant une nouvelle combinaison. Une description suppl & eacute;mentaire de 12 esp & egrave;ces pr & eacute;c & eacute;demment d & eacute;crites est & eacute;galement fournie : S. boulardi, S. bua Moyal et al., S. constantini, S. etchecopari, S. excelsa (Laporte), S. gnosia Moyal et al., S. mesophaea, S. congitae, S. mirifica, S. renibifida Berio, S. scotochroa (Hampson) et S. venata Fletcher. Des relev & eacute;s de plantes-h & ocirc;tes sont fournis pour dix esp & egrave;ces de Sciomesa, confirmant un mod & egrave;le de conservatisme de niche phylog & eacute;n & eacute;tique sur les plantes Poales de la famille des Cyperaceae. Des analyses phylog & eacute;n & eacute;tiques et mol & eacute;culaires de d & eacute;limitation des esp & egrave;ces ont & eacute;t & eacute; effectu & eacute;es sur un ensemble de donn & eacute;es mol & eacute;culaires multimarqueurs (quatre g & egrave;nes mitochondriaux et deux g & egrave;nes nucl & eacute;aires) comprenant 63 sp & eacute;cimens de Sciomesa, 24 esp & egrave;ces appartenant & agrave; d'autres genres de Sesamiina et un repr & eacute;sentant dans la sous-tribu des Apameina. Cet & eacute;chantillonnage englobe & eacute;galement des repr & eacute;sentants des trois esp & egrave;ces malgache (S. betschi Viette, S. janthina Viette et S. oberthueri Viette) dont l'appartenance au genre Sciomesa a & eacute;t & eacute; pr & eacute;c & eacute;demment remise en qu janthina au genre Sciomesa. Les r & eacute;sultats des analyses de d & eacute;limitation mol & eacute;culaire d'esp & egrave;ces soutiennent tr & egrave;s largement le statut d'esp & egrave;ce des taxa de Sciomesa & eacute;chantillonn & eacute;s, y compris pour S. mesophaea, l'esp & egrave;ce pr & eacute;sentant l'aire de r & eacute;partition la plus & eacute;tendue. Ces analyses r & eacute;v & egrave;lent & eacute;galement l'existence de trois potentielles nouvelles esp & egrave;ces, dont on ne peut pr & eacute;ciser le statut car les sp & eacute;cimens s & eacute;quenc & eacute;s l'ont & eacute;t & eacute; soit & agrave; un stade larvaire ou correspondent & agrave; des sp & eacute;cimens dont les genitalia n'ont pas & eacute;t & eacute; & eacute;tudi & eacute;s lors du pr & eacute;l & egrave;vement de leurs pattes.

The opening of habitats associated with the emergence of C4 grasslands during the Neogene had a massive influence on the evolution of plant and animal communities. Strikingly, the impacts of grassland expansion on species diversification in Africa, where the largest surface of grasslands and savannas in the world is located, are not well understood. To explore the impact of habitat opening, we investigate the evolution of noctuid stemborers, a group of moths mostly associated with open habitats, and whose diversity is centered in the Afrotropics. We generate a dated molecular phylogeny for ca 80% of the known stemborer species, and assess the role of habitat opening on the evolutionary trajectory of the group through a combination of parametric historical biogeography, ancestral character state estimation, life history traits and habitat-dependent diversification analyses. Our results support an origin of stemborers in Southern and East Africa ca 20 million years ago (Ma), with range expansions linked to the increased availability of open habitats to act as dispersal corridors, and closed habitats acting as potent barriers to dispersal. Early specialization on open habitats was maintained over time, with shifts towards closed habitats being rare and invariably unidirectional. Analyses of life history traits showed that habitat changes involved specific features likely associated with grassland adaptations, such as variations in larval behavior and color. We compare these findings to those previously inferred for an Afrotropical butterfly group that diversified roughly in parallel with the stemborers but distributed predominantly in closed habitats. Remarkably, these two groups show nearly opposite responses in relation to habitat specialization, whether in terms of biogeographical patterns, or in terms of rates of transition between open and closed habitats. We conclude that habitat opening played a major role in the evolutionary history of Afrotropical lineages through dispersal and adaptation linked to habitat shifts.

In this study, we reassess the phylogenetic relationships of the genus Sesamia Guenée, 1852 and examine in more detail the members of the nonagrioides species group, for which three distinct species complexes are identified. The calamistis subgroup comprises eight species, of which four new species are described: Sesamia kabirara Le Ru sp. nov., Sesamia kalale Le Ru sp. nov., Sesamia mapalense Le Ru sp. nov. and Sesamia teke Le Ru sp. nov. The incerta subgroup consists of 11 species, of which four new species are described: Sesamia kamba Le Ru sp. nov., Sesamia lalaci Le Ru sp. nov., Sesamia lusese Le Ru sp. nov. and Sesamia msowero Le Ru sp. nov. The nonagrioides subgroup comprises ten species of which two new species are described: Sesamia libode Le Ru sp. nov. and Sesamia satauensis Le Ru sp. nov. Phylogenetic and molecular species delimitation analyses of a multi-marker molecular dataset allow us to investigate and clarify the status of Sesamia species and species complexes. Our results yield a well-supported phylogenetic hypothesis for the genus, which supports the monophyletic nature of all but one species subgroup. The results of 16 distinct molecular species delimitation analyses show some levels of incongruence and, overall, a tendency towards over-splitting. We also present an updated list of species for the genus Sesamia and provide morphological keys based on male and female genitalia to determine the species group of any Sesamia species and to identify all species belonging to the nonagrioides species group.

Parasitoid wasps are haplodiploid, meaning that sperm stored by egg laying females are only used to produce daughters. Thus, the sex ratio of the offspring depends on the availability of sperm after mating. In these insects, males are sensitive to temperature at the pupal stage. This stress leads to subfertility due to a drastic reduction in the number of sperm produced and transferred to females. Experiments were conducted under controlled conditions on the parasitoid wasp Cotesia typhae (Hymenoptera, Braconidae), a natural enemy of the invading pest Sesamia nonagrioides (Lepidoptera, Noctuidae). At 25-27 degrees C, sperm production was measured for 7 days, and found to reach a plateau at the third day of adult life. It leads to a final amount around 25,000 sperm per male. A male can successfully inseminate at least 10 females, producing predominantly female offspring. Sperm production decreased significantly after 1 day of pupal exposure to heat at 34 or 36 degrees C and 7 days of cold at 0, 5 or 10 degrees C. This highlights that both cold and heat are stressful. After mating with one male treated at 10 or 34 degrees C, females store fewer sperm than the control, and produce fewer daughters. The sex ratio of the offspring is male biased when males experienced temperature stresses during development, like other parasitoid wasps. In the field, C. typhae populations would be affected by heat and cold, at least at the pupal stage. This lowers over wintering risk in case this biological agent was introduced in Europe. This risk is both economical, as companies seek to establish costly continuous production to sell beneficial insects, and ecological as the introduced population would not settle in the ecosystem. Lastly, the transport and storage of this insect of agronomic interest would need to consider temperature variations to ensure successful application.

Hymenopterans are haplodiploids and unlike most other Arthropods they do not possess sexual chromosomes. Sex determination typically happens via the ploidy of individuals: haploids become males and diploids become females. Arrhenotoky is believed to be the ancestral reproduction mode in Hymenopterans, with haploid males produced parthenogenetically, and diploid females produced sexually. However, a number of transitions towards thelytoky (diploid females produced parthenogenetically) have appeared in Hymenopterans, and in most cases populations or species are either totally arrhenotokous or totally thelytokous. Here we present the case of Cotesia typhae (Fernandez-Triana), a Braconidae that produces parthenogenetic females at a low frequency. The phenotyping of two laboratory strains and one natural population showed that this frequency is variable, and that this rare thelytokous phenomenon also happens in the wild. Moreover, mated females from one of the laboratory strains produce a few parthenogenetic daughters among a majority of sexual daughters. The analysis of daughters of heterozygous virgin females allowed us to show that a mechanism similar to automixis with central fusion is very likely at play in C. typhae. This mechanism allows some parts of the genome to remain heterozygous, especially at the chromosomes’ centromeres, which can be advantageous depending on the sex determination system involved. Lastly, in most species, the origin of thelytoky is either bacterial or genetic, and an antibiotic treatment as well as PCR experiments did not demonstrate a bacterial cause in C. typhae. The unusual case of low parthenogenetic frequency described in this species constitutes another example of the fascinating diversity of sex determination systems in Arthropods.

Hymenopterans are haplodiploids and unlike most other Arthropods they do not possess sexual 13 chromosomes. Sex determination typically happens via the ploidy of individuals: haploids become 14 males and diploids become females. Arrhenotoky is believed to be the ancestral reproduction mode 15 in Hymenopterans, with haploid males produced parthenogenetically, and diploid females produced 16 sexually. However, a number of transitions towards thelytoky (diploid females produced 17 parthenogenetically) have appeared in Hymenopterans, and in most cases populations or species are 18 either totally arrhenotokous or totally thelytokous. Here we present the case of Cotesia typhae 19 (Fernandez-Triana), a Braconidae that produces parthenogenetic females at a low frequency. The 20 phenotyping of two laboratory strains and one natural population showed that this frequency is 21 variable, and that this rare thelytokous phenomenon also happens in the wild. Moreover, mated 22 females from one of the laboratory strains produce a few parthenogenetic daughters among a 23 majority of sexual daughters. The analysis of daughters of heterozygous virgin females allowed us to 24 show that a mechanism similar to automixis with central fusion is very likely at play in C. typhae . This 25 mechanism allows some parts of the genome to remain heterozygous, especially at the 26 chromosomes’ centromeres, which can be advantageous depending on the sex determination 27 system involved. Lastly, in most species, the origin of thelytoky is either bacterial or genetic, and an 28 antibiotic treatment as well as PCR experiments did not demonstrate a bacterial cause in C. typhae . 29 The unusual case of low parthenogenetic frequency described in this species constitutes another 30 example of the fascinating diversity of sex determination systems in Arthropods.

SummaryIn this study, 31 species of noctuid stemborers belonging to the genus Sesamia Guenée, 1852 (Lepidoptera: Noctuidae: Noctuinae: Apameini: Sesamiina) are reviewed. All these species are assigned to the Sesamia cretica group sensu Tams & Bowden (1953). Based on genitalic characters, several subgroups are hereby defined. Nine species belong to a species complex defined as the Sesamia albivena Hampson, 1902 subgroup; it consists of S. albivena, S. mocoensis Tams & Bowden, 1953, n. stat., S. sudanensis Tams & Bowden, 1953, n. stat. S. taenioleuca (Wallengren, 1863), and five new species that are described (S. aethiopica Le Ru n. sp. from Ethiopia, S. kafulo Le Ru n. sp. from Botswana and Zambia, S. kavirondo Le Ru n. sp. from Kenya and Uganda, S. maloukou Le Ru n. sp. from Republic of Congo, and S. soyema Le Ru n. sp. from Ethiopia). Four species belong to a species complex defined as the Sesamia cretica subgroup; this encompasses S. cretica, S. rufescens Hampson, 1910, and two new species that are described (S. ihambane Le Ru n. sp. from Mozambique and Tanzania and S. kikuyuensis Le Ru n. sp. from Kenya); two new synonyms are introduced for Sesamia cretica: Nonagria uniformis Dudgeon, 1905 n. syn. and Sesamia griselda Warren, 1913, n. syn. Ten species belong to a species complex defined as the Sesamia fuscifrontia Hampson, 1914 subgroup; this includes S. fuscifrontia, S. geyri (Strand, 1915) and eight new species that are described (S. babati Le Ru n. sp. from Tanzania, S. babessi Le Ru n. sp. from Cameroon and Zambia, S. mabira Le Ru n. sp. from Uganda, S. nangaensis Le Ru n. sp. from Cameroon and Republic of Congo, S. rungwa Le Ru n. sp. from Tanzania, S. simillima Le Ru n. sp. from Benin, Cameroon, Kenya and Uganda, S. taveta Le Ru n. sp. from Kenya and S. ulaukae Le Ru n. sp. from Ethiopia). One species belongs to a species complex defined as the Sesamia salama Le Ru n. sp. subgroup; this consists of S. salama Le Ru n. sp. from Kenya and another undescribed Sesamia species from South Africa. One species belongs to a species complex defined as the Sesamia viettei Rungs, 1954 subgroup. Six species belong to a species complex defined as the Sesamia wiltshirei Rungs, 1963 subgroup; this groups S. wiltshirei and five new species that are described (S. djenoensis Le Ru n. sp. from Republic of Congo, S. inexpectata Le Ru n. sp. from South Africa and Zambia, S. lefini Le Ru n. sp. from Republic of Congo, S. echinochloa Le Ru n. sp. from Botswana, Kenya, Mozambique, South Africa, Tanzania and Zambia and S. rindini Le Ru n. sp. from Tanzania). A supplemental description of the previously described species is also provided. Novel host plant records are also provided for 11 species of the S. cretica group. To complement the morphological study, both phylogenetic and molecular species delimitation analyses were carried out on a multimarker (four mitochondrial and two nuclear genes) molecular dataset encompassing 144 specimens representing 35 species (including 25 species from the S. cretica group). Molecular analyses provide a well-supported phylogenetic framework for the species of interest, which are all recovered monophyletic. Molecular species delimitation analyses also support the species status of almost all sampled species. Interestingly, the inferred tree indicates that the S. cretica group and the S. fuscifrontia subgroup are both paraphyletic; this indicates that, while highly informative, the chosen genitalic characters in Sesamia are not all synapomorphies.

The success of biological bontrol (BC) introductions can be enhanced by considering theory and knowledge of biological systems. The gregarious braconid parasitoid Cotesia sesamiae (Cameroon) is one of the best studied biological control agent from the perspective of molecular ecology. Its evolutionary adaptation to the target host involves symbiotic partners. Polydnaviruses are responsible for immune and developmental adaptations whereas Wolbachia bacteria may reinforce this local adaptation though genetic isolation mechanisms. The noctuid Busseola fusca is a major stemborer pest of maize in sub-Saharan Africa. In contrast to eastern Africa, C. sesamiae is rarely found on B. fusca in western Africa. It is however often obtained from other stemborer species feeding on wild grasses. A biological control project was launched in 2006-2007 by introducing to Cameroon seven crosses of Kenyan populations of C. sesamiae collected in different ecozones. They included populations adapted to B. fusca that develop on maize as well as populations adapted to other hosts feeding on wild plants to allow carryover between cropping seasons. Wolbachia strains responsible for cytoplasmic reproductive incompatibilities with endemic strains were included in the crosses to limit genetic exchanges between introduced and endemic C. sesamiae and preserve genetic adaptation to B. fusca of the introduced populations, while at the same time preserving their ability to survive on wild plants. Six post release surveys were carried out on maize from 2007 to 2013, and on wild grasses in 2013. A total of 393 C. sesamiae individuals, each from one cocoon mass, were genotyped for 11 microsatellite loci. Multidimensional scaling analysis, STRUCTURE and GENE CLASS analyses assigned almost all the parasitoids recovered from maize to those introduced from Kenya. The introduced strains were also recovered from wild host plants with little genetic exchanges with endemics. Each population remained strongly associated with its original Wolbachia component, suggesting that Wolbachia may contribute to genetic isolation between endemics and introduced populations in wild host plants when maize is absent, thereby conciliating biological control success and safety.

Most endogenous viruses, an important proportion of eukaryote genomes, are doomed to slowly decay. Little is known, however, on how they evolve when they confer a benefit to their host. Bracoviruses are essential for the parasitism success of parasitoid wasps, whose genomes they integrated ~103 million years ago. Here we show, from the assembly of a parasitoid wasp genome, for the first time at a chromosomal scale, that symbiotic bracovirus genes spread to and colonized all the chromosomes. Moreover, large viral clusters are stably maintained suggesting strong evolutionary constraints. Genomic comparison with another wasps revealed that this organization was already established ~53 mya. Transcriptomic analyses highlight temporal synchronization of viral gene expression, leading to particle production. Immune genes are not induced, however, indicating the virus is not perceived as foreign by the wasp. This recognition suggests that no conflicts remain between symbiotic partners when benefits to them converge.

Dissecting the genetic basis of intraspecific variations in life history traits is essential to understand their evolution, notably for potential biocontrol agents. Such variations are observed in the endoparasitoid Cotesia typhae (Hymenoptera: Braconidae), specialized on the pest Sesamia nonagrioides (Lepidoptera: Noctuidae). Previously, we identified two strains of C. typhae that differed significantly for life history traits on an allopatric host population. To investigate the genetic basis underlying these phenotypic differences, we used a quantitative trait locus (QTL) approach based on restriction site‐associated DNA markers. The characteristic of C. typhae reproduction allowed us generating sisters sharing almost the same genetic content, named clonal sibship. Crosses between individuals from the two strains were performed to generate F2 and F8 recombinant CSS. The genotypes of 181 clonal sibships were determined as well as the phenotypes of the corresponding 4,000 females. Informative markers were then used to build a high‐quality genetic map. These 465 markers spanned a total length of 1,300 cM and were organized in 10 linkage groups which corresponded to the number of C. typhae chromosomes. Three QTLs were detected for parasitism success and two for offspring number, while none were identified for sex ratio. The QTLs explained, respectively, 27.7% and 24.5% of the phenotypic variation observed. The gene content of the genomic intervals was investigated based on the genome of C. congregata and revealed 67 interesting candidates, as potentially involved in the studied traits, including components of the venom and of the symbiotic virus (bracovirus) shown to be necessary for parasitism success in related wasps.

In this study, we review six morphologically similar species of Sesamia Guenee, 1852 from Africa, including three new species that are described: Sesamia corymbosus Le Ru n. sp., S. schoenoplectus Le Ru n. sp. and S. temberma Le Ru n. sp. These six species belong to a species complex that we hereby define as the Sesamia coniota group. Host plants of four species are recorded: Schoenoplectus corymbosus (Roth ex Roem. & Schult.) J. Raynal. for S. corymbosus and S. schoenoplectus, Echinochloa pyramidalis (Lam.) Hitschc. & Chase, Eriochloa meyeriana (Nees) Pilg., Schoenoplectus corymbosus and Typha latifolia L. for S. jansei, Tams & Bowden, 1953 and Pennisetum sp. for S. temberma. We also conduct molecular phylogenetic analyses on a multimarker (four mitochondrial and two nuclear genes) molecular dataset encompassing 36 specimens (including 32 specimens belonging to the S. coniota group). Molecular analyses allow assessing the phylogenetic relationships of five out of six species of the group.

Summary In this study, we review six morphologically similar species of Sesamia Guenée, 1852 from Africa, including three new species that are described: Sesamia corymbosus Le Ru n. sp., S. schoenoplectus Le Ru n. sp. and S. temberma Le Ru n. sp. These six species belong to a species complex that we hereby define as the Sesamia coniota group. Host plants of four species are recorded: Schoenoplectus corymbosus (Roth ex Roem. & Schult.) J. Raynal. for S. corymbosus and S. schoenoplectus, Echinochloa pyramidalis (Lam.) Hitschc. & Chase, Eriochloa meyeriana (Nees) Pilg., Schoenoplectus corymbosus and Typha latifolia L. for S. jansei, Tams & Bowden, 1953 and Pennisetum sp. for S. temberma. We also conduct molecular phylogenetic analyses on a multimarker (four mitochondrial and two nuclear genes) molecular dataset encompassing 36 specimens (including 32 specimens belonging to the S. coniota group). Molecular analyses allow assessing the phylogenetic relationships of five out of six species of the group.

In this study, we review six morphologically similar species of Sesamia Guenée, 1852 from Africa, including three new species that are described: Sesamia corymbosus Le Ru n. sp., S. schoenoplectus Le Ru n. sp. and S. temberma Le Ru n. sp. These six species belong to a species complex that we hereby define as the Sesamia coniota group. Host plants of four species are recorded: Schoenoplectus corymbosus (Roth ex Roem. & Schult.) J. Raynal. for S. corymbosus and S. schoenoplectus, Echinochloa pyramidalis (Lam.) Hitschc. & Chase, Eriochloa meyeriana (Nees) Pilg., Schoenoplectus corymbosus and Typha latifolia L. for S. jansei, Tams & Bowden, 1953 and Pennisetum sp. for S. temberma. We also conduct molecular phylogenetic analyses on a multimarker (four mitochondrial and two nuclear genes) molecular dataset encompassing 36 specimens (including 32 specimens belonging to the S. coniota group). Molecular analyses allow assessing the phylogenetic relationships of five out of six species of the group.

The genusPoeonomaTams & Bowden, 1953 is revised to include two speciesP. serrata(Hampson, 1910) andP. ugandensisLe Run. sp., from the Congolian bioregion. Several new synonyms are introduced forPoeonoma serrata:Conicofrontia sjoestedtiAurivillius, 1925n. syn. forPoeonoma serrata(Hampson, 1910),Poeonoma similisTams & Bowden, 1953n. syn. forPoeonoma serrata(Hampson, 1910), andPoeonoma nigribasisLaporte, 1974n. syn. forPoeonoma serrata(Hampson, 1910). A new genus,NyalutemeLe Run. gen., is proposed for two species formerly included inPoeonoma,N. acantha(Tams & Bowden, 1953)n. comb. andN. inermis(Laporte, 1973)n. comb., andN. nigraLe Run. sp., described from the Congolian bioregion. Host-plants for three of the species are recorded:Pennisetum purpureumSchumach. forP. serrataandP. ugandensis, andMiscanthus violaceus(K. Schum) Pilg. forN. nigra. A key to species, descriptions, illustrations of adults and genitalia, and distribution maps are included. Results of previously published phylogenetic analyses also allow the status of the new genus to be confirmed and to infer the phylogenetic placement of the two genera.

SummaryWith a species count reaching almost 100 species, the genus Acrapex is the most diverse genus of sesamiine stemborers (Lepidoptera: Noctuidae: Noctuinae: Apameini: Sesamiina). Acrapex species are mostly distributed in the Afrotropics and consist of several large clades corresponding to distinct species complexes. In this study, 45 morphologically similar species of Acrapex from sub-Saharan Africa are reviewed, including 22 new species that are described: Acrapex alemura n. sp., A. barnsi n. sp., A. capelongo n. sp., A. congoensis n. sp., A. elgona n. sp., A. elisabethiana n. sp., A. eucantha n. sp., A. grandis n. sp., A. igominyi n. sp., A. inexpectata n. sp., A. ketoma n. sp., A. lilomwi n. sp., A. mafinga n. sp., A. makete n. sp., A. marungu n. sp., A. mazoe n. sp., A. mlanje n. sp., Acrapex muchinga n. sp., A. ngorongoro n. sp., A. obscura n. sp., A. ruiru n. sp. and A. wittei n. sp. Supplemental descriptions for previously described species are provided as well. These 45 species are assigned to the newly defined Acrapex aenigma species group. We also conduct molecular phylogenetic analyses and molecular species delimitation analyses on a multi-marker (four mitochondrial and two nuclear genes) molecular dataset encompassing 304 specimens (including 256 Acrapex specimens from 54 species of which 16 species belong to the A. aenigma group). Molecular phylogenetics analyses recover well-supported relationships within Acrapex and support the monophyly of the newly defined group. Results of molecular species delimitation analyses are mostly congruent and tend to corroborate the status of the sampled Acrapex species. Consistent with what has been previously found in other studies, the comparison of results from distinct methods and settings for molecular species delimitation analyses allows us to assess species boundaries with more confidence.

Summary With a species count reaching almost 100 species, the genus Acrapex is the most diverse genus of sesamiine stemborers (Lepidoptera: Noctuidae: Noctuinae: Apameini: Sesamiina). Acrapex species are mostly distributed in the Afrotropics and consist of several large clades corresponding to distinct species complexes. In this study, 45 morphologically similar species of Acrapex from sub-Saharan Africa are reviewed, including 22 new species that are described: Acrapex alemura n. sp., A. barnsi n. sp., A. capelongo n. sp., A. congoensis n. sp., A. elgona n. sp., A. elisabethiana n. sp., A. eucantha n. sp., A. grandis n. sp., A. igominyi n. sp., A. inexpectata n. sp., A. ketoma n. sp., A. lilomwi n. sp., A. mafinga n. sp., A. makete n. sp., A. marungu n. sp., A. mazoe n. sp., A. mlanje n. sp., Acrapex muchinga n. sp., A. ngorongoro n. sp., A. obscura n. sp., A. ruiru n. sp. and A. wittei n. sp. Supplemental descriptions for previously described species are provided as well. These 45 species are assigned to the newly defined Acrapex aenigma species group. We also conduct molecular phylogenetic analyses and molecular species delimitation analyses on a multi-marker (four mitochondrial and two nuclear genes) molecular dataset encompassing 304 specimens (including 256 Acrapex specimens from 54 species of which 16 species belong to the A. aenigma group). Molecular phylogenetics analyses recover well-supported relationships within Acrapex and support the monophyly of the newly defined group. Results of molecular species delimitation analyses are mostly congruent and tend to corroborate the status of the sampled Acrapex species. Consistent with what has been previously found in other studies, the comparison of results from distinct methods and settings for molecular species delimitation analyses allows us to assess species boundaries with more confidence.