ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCountercurrent Distribution of Phosvitin*Chi-Ching Mok, Cynthia T. Grant, and George TaborskyCite this: Biochemistry 1966, 5, 8, 2517–2523Publication Date (Print):August 1, 1966Publication History Published online1 May 2002Published inissue 1 August 1966https://doi.org/10.1021/bi00872a005Request reuse permissionsArticle Views34Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

Ten antigens have been distinguished in the sera of cocks and non-laying hens by immunoelectrophoretic analysis (IEA) against homologous rabbit antisera. An additional antigen has been found in the sera of laying hens by IEA against anti-laying-hen serum.Four of the serum antigens obtained by IEA have been correlated electrophoretically with the filter paper electrophoretic fractions serum albumin, serum α2-globulin, serum β-globulin, and serum γ-globulin.Serum albumin, serum α1-globulin, serum β-globulin, and serum γ-globulin have been identified immunologically with alpha-livetin, a livetin antigen (livetin 2), gamma1-livetin and gamma2-livetin respectively. Another antigen of cock serum and hen serum has been identified by IEA with a livetin antigen detected by IEA of livetin against anti-cock, anti-hen, and anti-yolk sera.

A phosvitin has been prepared from the serum of estrogenized laying hens and partially characterized on the basis of its contents of N (12.3%), P (10.1%), serine (31.0%), and tryptophan (0.56%). Examination of this serum phosvitin and a yolk phosvitin preparation for N-terminal groups yielded identical chromatographic patterns. Alanine was the major N-terminal amino acid with minor amounts of N-terminal lysine. The sedimentation constant [Formula: see text], weight-average molecular weight 4.2 × 104(Archibald method), and number-average molecular weight 4.0 × 104(calculated from the tryptophan content) of the serum phosvitin were similar to those of the phosvitin prepared from egg yolk by the same preparative technique. The two preparations behaved similarly when subjected to zone electrophoresis on paper.Zone electrophoretic evidence is submitted for the presence of phosvitin in the supernatant from dilution precipitation of crude lipophosphoprotein from laying hen's serum as well as in the precipitate itself.