Molecular MicrobiologyVolume 9, Issue 1 p. 221-223 The general amivno acid control regulates MET4, which encodes a methionine-pathway-specific transcriptional activator of Saccharomyces cerevisiae Harry A. Mountain, Harry A. Mountain Department of Microbiology, Umeå University, S-901 87 Umeå, Sweden. Division of Biology, Schooi of Sciences, Staffordshire University, Coliege Road, Stoke-on-Trent STH 2DE, UK.Search for more papers by this authorAnders S. Byström, Anders S. Byström Department of Microbiology, Umeå University, S-901 87 Umeå, Sweden.Search for more papers by this authorChristopher Korch, Corresponding Author Christopher Korch Department of Microbiology, Umeå University, S-901 87 Umeå, Sweden.*For correspondence: Imperial Holly Research and Development Center, 5320 Mark Dabling Boulevard, Colorado Springs, Colorado 80918, USA. Tel. (719) 471 0123; Fax (719) 599 7006.Search for more papers by this author Harry A. Mountain, Harry A. Mountain Department of Microbiology, Umeå University, S-901 87 Umeå, Sweden. Division of Biology, Schooi of Sciences, Staffordshire University, Coliege Road, Stoke-on-Trent STH 2DE, UK.Search for more papers by this authorAnders S. Byström, Anders S. Byström Department of Microbiology, Umeå University, S-901 87 Umeå, Sweden.Search for more papers by this authorChristopher Korch, Corresponding Author Christopher Korch Department of Microbiology, Umeå University, S-901 87 Umeå, Sweden.*For correspondence: Imperial Holly Research and Development Center, 5320 Mark Dabling Boulevard, Colorado Springs, Colorado 80918, USA. Tel. (719) 471 0123; Fax (719) 599 7006.Search for more papers by this author First published: July 1993 https://doi.org/10.1111/j.1365-2958.1993.tb01684.xCitations: 3 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume9, Issue1July 1993Pages 221-223 RelatedInformation

The MET14 gene of Saccharomyces cerevisiae, encoding APS kinase (ATP: adenylylsulfate-3'-phosphotransferase, EC 2.7.1.25), has been cloned. The nucleotide sequence predicts a protein of 202 amino acids with a molecular mass of 23060 dalton. Translational fusions of MET14 with the beta-galactosidase gene (lacZ) of Escherichia coli confirmed the results of primer extension and Northern blot analyses indicating that the ca. 0.7 kb mRNA is transcriptionally repressed by the presence of methionine in the growth medium. By primer extension the MET14 transcripts were found to start between positions -25 and -45 upstream of the initiator codon. Located upstream of the MET14 gene is a perfect match (positions -222 to -229) with the previously proposed methionine-specific upstream activating sequence (UAS(Met)). This is the same as the consensus sequence of the Centromere DNA Element I (CDEI) that binds the Centromere Promoter Factor I (CPFI) and of two regulatory elements of the PHO5 gene to which the yeast protein PHO4 binds. The human oncogenic protein c-Myc also has the same recognition sequence. Furthermore, in the 270 bp upstream of the MET14 coding region there are several matches with a methionine-specific upstream negative (URS(Met)) control element. The significance of these sequences was investigated using different upstream deletion mutations of the MET14 gene which were fused to the lacZ gene of E. coli and chromosomally integrated. We find that the methionine-specific UAS(Met) and one of the URS(Met) lie in regions necessary for strong activation and weak repression of MET14 transcription, respectively. We propose that both types of control are exerted on MET14.