To compare clinical outcomes after fresh embryo transfer on laser-assisted zona pellucida opening (LAO) versus thinning (LAT) according to maternal age in patients with repeated implantation failure (RIF). A retrospective study of 509 (n = 458 patients) in vitro fertilization/intracytoplasmic sperm injection cycles was investigated from January 2013 to July 2017. We compared whether LAT and LAO affect the clinical outcomes in young maternal age (YMA, <38 years) and old maternal age (OMA, ≥38 years) patient groups with ≥2 of RIF. The cycles with an oocyte donation, oocyte activation, genetic diagnosis, and that used surrogate mothers were excluded. Participants were divided into 4 groups according to maternal age and the two types of laser-assisted hatching (YMA: LAT, n = 119 vs. LAO, n = 179 and OMA: LAT, n = 72 vs. LAO, n = 139). LAO was opened using 3-4 laser shot in the zona pellucida. The laser thinning was performed by making 3-4 holes without reaching the inner membrane at a depth of 60%-80% of the zona pellucida thickness. Laser-assisted hatching was performed 2 hours before the embryo transfer. The characteristics of patients did not differ significantly among the groups (p > 0.05), with the exception of mixed factor infertility, which was more common in the LAT group than in the LAO group among patients <38 years of age (10.1% vs. 2.8%, p = 0.008). We also observed similar rates of clinical pregnancy (27.7% vs. 24.6%, p = 0.543; 16.7% vs. 18.7, p = 0.715), ongoing pregnancy (22.7% vs. 21.8%, p = 0.854; 8.3% vs. 15.1, p = 0.163), abortion (18.2% vs. 11.4%, p = 0.397; 50.0% vs. 19.2, p = 0.052), implantation (17.2% vs. 16.5%, p = 0.811; 11.1% vs. 11.2, p = 0.990), and twin pregnancy (5.0% vs. 5.6%, p = 0.498; 0.0% vs. 2.2, p = 0.553) between LAT and LAO in the YMA or the OMA group. Clinical outcomes were similar between LAT and LAO in the YMA or the OMA group. However, the OMA group who underwent LAO tended to have a lower abortion rate. Further study is necessary to confirm these results in a larger population.