A new method for monitoring phospholipase A (PLA) activity in venom extracts is under investigation to assess its suitability as a stability indicating assay. Lyophilized honey bee venom (HBV) and yellow jacket venom (YJV) samples were reconstituted to 100μg/mL and stored under various conditions; recommended storage (5oC ± 3oC), accelerated (25oC ± 2oC), and stressed (40oC ± 2oC). Testing was performed at various time-points using the alternative PLA assay. For each time-point, freshly reconstituted samples acted as controls. Both venom species demonstrated a temperature-dependent change in PLA activity. For example, when HBV samples were reconstituted in albumin buffered saline and kept under the recommended storage condition (5oC ± 3oC) there was essentially no change in PLA activity through 6 months. However, over the same time period under the accelerated condition (25oC ± 2oC) there was a 40% or greater decline in measured PLA activity. An even greater change was observed under the stressed condition (40oC ± 2oC), where at least a 60% loss of PLA activity was observed. The trend seen for PLA activity in YJV samples was similar, but even more pronounced for the accelerated and stressed conditions over the same time frame. Forced degradation studies have demonstrated the stability indicating potential of a new method for measuring PLA activity in venom extracts.