Dorsal root ganglia from rats were incubated with 3-O-methyl-[14C]glucose, and [3H]leucine in the presence or absence of insulin in order to determine whether insulin influences the uptake of glucose and amino acids by the cells of the ganglion. No effect was detected. A significant proportion (38%) of the uptake of [3H]leucine was shown to be inhibited by ouabain and therefore energy dependent, utilizing Na+K+-ATPase. The activity of this enzyme is known to be impaired in dorsal root ganglia in diabetic rats, as is the uptake of amino acids; these phenomena are therefore unlikely to be due to a direct effect of reduced circulating insulin levels. The relevance of these findings to theories as to the causation of diabetic neuropathy is discussed.

Streptozotocin (STZ)-induced diabetes in the rat causes a significant reduction in ouabain-sensitive Na,K-ATPase pumping activity measured by 86Rb+ influx, in sciatic endoneurium (by 54%) and dorsal root ganglia (by 22%). For endoneurium, the change is similar to that of ouabain-sensitive enzymatic Na,K-ATPase activity (42%), but in dorsal root ganglia, the decrease in enzymatic Na,K-ATPase activity was much greater. 86Rb+ efflux from dorsal root ganglia showed no difference between diabetic and control animals, confirming that the abnormal 86Rb+ influx reflects Na,K-ATPase function and not abnormal membrane permeability. The significance of these findings to pathogenetic mechanisms in diabetic neuropathy is discussed.

Conference Article| October 01 1988 Lack of correlation between enzymic Na+, K+-ATPase activity and 86Rb+ uptake in peripheral nerve tissue of diabetic rats N. J. PATEL; N. J. PATEL 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U.K. Search for other works by this author on: This Site PubMed Google Scholar J. G. LLEWELYN; J. G. LLEWELYN 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U.K. Search for other works by this author on: This Site PubMed Google Scholar D. W. WRIGHT; D. W. WRIGHT 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U.K. Search for other works by this author on: This Site PubMed Google Scholar P. K. THOMAS P. K. THOMAS 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1988) 16 (5): 878. https://doi.org/10.1042/bst0160878 Article history Received: April 06 1988 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation N. J. PATEL, J. G. LLEWELYN, D. W. WRIGHT, P. K. THOMAS; Lack of correlation between enzymic Na+, K+-ATPase activity and 86Rb+ uptake in peripheral nerve tissue of diabetic rats. Biochem Soc Trans 1 October 1988; 16 (5): 878. doi: https://doi.org/10.1042/bst0160878 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search © 1988 Biochemical Society1988 Article PDF first page preview Close Modal You do not currently have access to this content.

Conference Article| August 01 1987 Protein synthesis and amino acid uptake by dorsal root ganglia from diabetic rats N. J. PATEL; N. J. PATEL 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PN, U.K. Search for other works by this author on: This Site PubMed Google Scholar J. G. LLEWELYN; J. G. LLEWELYN 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PN, U.K. Search for other works by this author on: This Site PubMed Google Scholar D. W. WRIGHT; D. W. WRIGHT 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PN, U.K. Search for other works by this author on: This Site PubMed Google Scholar P. K. THOMAS P. K. THOMAS 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PN, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1987) 15 (4): 695–696. https://doi.org/10.1042/bst0150695 Article history Received: November 26 1986 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation N. J. PATEL, J. G. LLEWELYN, D. W. WRIGHT, P. K. THOMAS; Protein synthesis and amino acid uptake by dorsal root ganglia from diabetic rats. Biochem Soc Trans 1 August 1987; 15 (4): 695–696. doi: https://doi.org/10.1042/bst0150695 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: STZ, streptozotocin, TCA, trichloracetic acid © 1987 Biochemical Society1987 Article PDF first page preview Close Modal You do not currently have access to this content.