Glutamine is the most abundant plasma amino acid and many cancers show altered glutamine metabolism. We evaluated glutamine uptake and metabolism in gliomas using PET imaging and biochemical approaches. We demonstrate that glutamine is a key TCA cycle anaplerotic substrate and is metabolized to generate 2-HG in IDH1-mutant gliomas. PET imaging with18F-labeled glutamine (18F-FGln) showed high uptake in gliomas in vivo but low background uptake in the surrounding brain in RCAS-PDGF/PTEN null and IDH1-mutant glioma animal models, facilitating clear tumor delineation in contrast to that seen with 18F-FDG. We did not observe 18F-FGln uptake in animals with neuroinflammation or animals with a disrupted BBB. Further, 18F-FGln uptake was specifically reduced on chemo/radiation therapy. Finally, 18F-FGln showed high avidity in human glioma with low uptake in the surrounding brain. These data suggest that 18F-FGln is specifically taken up by gliomas, can be used to assess the metabolic state of gliomas in vivo and may serve as a valuable tool in the clinical management of gliomas.

0533 PURPOSE: To examine the effects of the Curves fitness and diet program on muscular strength, muscular endurance, and maximal aerobic capacity. METHODS: 123 sedentary women (38.7 ± 8 yr; 93.2 ± 19 kg; 44.8 ± 4.8% body fat) participated in a 14- wk exercise and diet program. Subjects were randomly assigned to an exercise and no diet group (END); an exercise and high calorie mixed diet (2,600 kcals/d for 2 wks at 55% C, 15% P, 30% F; 8 wks at 40% C, 30% P, 30% F; 4 wks at 55% C, 15% P, 30% F) group (HCD); or, a low calorie high carbohydrate (HCHO), high protein (HP), or very high protein (VHP) diet group. Subjects consumed 1,200 kcal/d for 2-wks and 1,600 kcal/d for 8 wks. Subjects then ingested 2,600 kcal/d and 1,200 kcal/d diet at 3/2, 3/2, 5/2, & 10/2 day intervals in an attempt to maintain weight loss. Diets were standardized with 30% dietary fat with carbohydrate intake ranging from 40–55% on the HCD and HCO diets and protein intake ranging from 50–63% on the HP and VHP diets. Subjects participated in a supervised 30-min resistance training circuit program combined with calisthenic exercises 3-d per week. At 0, 10, and 14 weeks, subjects performed 1RM bench press and leg press, 80% of 1RM maximal repetition tests on the bench press and leg press, and a maximal cardiopulmonary exercise test. Repeated measures ANOVA results are presented as means ± SD from baseline. RESULTS: Training significantly increased bench press 1RM (2.37 ± 3.8 kg, p<0.006; 0.04 ± 0.04 kg/kg, p<0.001) and leg press 1RM (15.5 ± 38 kg, p<0.002; 0.27 ± 0.39 kg/kg, p<0.001) [10–15% gain in strength]. Bench press lifting volume (80% weight × repetitions) was unchanged (9.1 ± 126 kg, p = 0.20; 0.3 ± 1.4 kg/kg, p = 0.60). However, significant interactions (p<0.05) were observed indicating the END group experienced greater gains in bench press lifting volume. Leg press lifting volume significantly increased in all groups (325 ± 1,521 kg, p = 0.03; 4.1 ± 14 kg/kg, p = 0.006) with no significant differences among groups. Relative maximal oxygen uptake was significantly increased in all groups by about 7% (1.6 ± 3.5 ml/kg/min, p<0.002) primarily due to a significant weight loss. Resting heart (−4.2 ± 14.0 bpm, p<0.01), systolic blood pressure (−3.2 ± 13 mmHg, p<0.001), and diastolic blood pressure (−2.1 ± 10 mmHg, p<0.03) decreased. CONCLUSIONS: The Curves fitness program promotes increases in muscular strength, muscular endurance, and maximal aerobic capacity while decreasing resting heart rate and blood pressure. These findings indicate that this program appears to be an effective and appropriate level exercise program for this population.