Polydimethylsiloxane (PDMS) is a commonly used material for the design of new cell culture devices. Typically, molded PDMS is bonded to glass or another PDMS layer; however, it is challenging to form high-strength bonds with polymers commonly used for cell culture-ware, such as polystyrene (PS). Therefore, a robust PDMS–PS bonding method would facilitate a wider use of PDMS in tissue culture applications. Herein, we develop an irreversible, stable, and consistent PDMS–PS bonding protocol. We show that this method is superior to other currently available methods because of its bonding strength, reusability, and reliability. Furthermore, we demonstrate the utility of this bonding process in fabricating our Heart-Dyno device, a 96-well-based platform for culturing cardiac organoids. This bonding process has been successfully used over the past 2 years, with hundreds of plates manufactured for drug screening applications. Others may also have similar applications where this method may facilitate the fabrication of more robust PDMS–PS devices.
This study investigated the perforation resistance behaviour of metal–plastic laminates (MPLs) when they are indented by different nose shapes. Aluminium (Al) and HDPE (high-density polyethylene) layers were bonded with a suitable adhesive in an alternative manner to prepare bilayer and trilayer MPL configurations. Quasi-static perforation experiments were performed with hemispherical, conical and blunt indenters. The effects of nose shape, layer configuration and adhesive on the force–deformation profile, perforation resistance capacity and failure mechanisms were evaluated. The results indicate that for a monolithic layer, the blunt indenter showed the highest perforation energy capacity. The conical and blunt indenters facing Al backed by HDPE gave higher perforation energy. The hemispherical indenter facing HDPE backed by Al was found to be more effective in perforation resistance. Trilayer Al–HDPE–Al showed higher perforation resistance than HDPE–Al–HDPE. Circumferential cracking, radial symmetric cracking and shear plugging were the main failure modes for Al under hemispherical, conical and blunt indenters, respectively. The adhesive contributed to an increase in the perforation energy and peak force to failure in laminates. The adhesive was shown to detach from the Al surface after Al fracturing through crack propagation, and this effect was more pronounced when the indenter faced HDPE at the front of the laminate.
This paper presents recycling of selective laser sintering (SLS) waste nylon into printable filaments and parts reinforced with Mg particles. Waste nylon and waste–Mg powder mixture with 2%, 4%, and 8% Mg to nylon were extruded into the filaments. Moisture absorption, differential scanning calorimetry, and melt flow index experiments were conducted to determine the thermal characteristics, while tensile and flexural tests were conducted to evaluate mechanical properties and failure mechanisms. The results were compared with off-the-self (OTS) nylon. Waste powder was found to be extrudable and printable as FFF filament. Waste filament diameter closely matched standard filament size, while exhibiting reduced moisture absorption. High melting and crystallisation temperature for the waste nylon demonstrated a degradation of the plastic during the SLS process. Young’s modulus and ultimate tensile strength for the waste filament increased by 1.6-fold compared to that for OTS, while Mg-composite filament surpassed the waste and OTS. Waste and Mg composite dog bone results showed an increase in strength and stiffness, but the ductility deteriorated. Both flexural strength and modulus for the waste nylon increased by 13% and 26%, respectively, over OTS, and the addition of Mg enhanced flexural strength by up to 5-fold at 8% Mg over the waste. Printed surface topography demonstrated that the waste and Mg composite filaments can print the parts with desired geometric shapes and acceptable surface texture. The findings showed that recycling waste SLS powder into FFF prints would be a viable and useful alternative to disposal, given its abundance.