The Planetary Society's Phobos Living Interplanetary Flight Experiment (Phobos LIFE) flew in the sample return capsule of the Russian Federal Space Agency's Phobos Grunt mission and was to have been a test of one aspect of the hypothesis that life can move between nearby planets within ejected rocks. Although the Phobos Grunt mission failed, we present here the scientific and engineering design and motivation of the Phobos LIFE experiment to assist with the scientific and engineering design of similar future experiments. Phobos LIFE flew selected organisms in a simulated meteoroid. The 34-month voyage would have been the first such test to occur in the high-radiation environment outside the protection of Earth's magnetosphere for more than a few days. The patented Phobos LIFE "biomodule" is an 88 g cylinder consisting of a titanium outer shell, several types of redundant seals, and 31 individual Delrin sample containers. Phobos LIFE contained 10 different organisms, representing all three domains of life, and one soil sample. The organisms are all very well characterized, most with sequenced genomes. Most are extremophiles, and most have flown in low Earth orbit. Upon return from space, the health and characteristics of organisms were to have been compared with controls that remained on Earth and have not yet been opened.

A life-detection system based on the expectation that any viable organism will utilize stereoisomers of a given compound asymmetrically is examined. Aqueous extracts of common soil, Mars regolith simulant JSC Mars-1, and suspensions of E. coli and S. cerevisiae were incubated with stereoisomer pairs. The enantiomeric pairs were either D- and L-glucose or a pair of chiral tetrasaccharides. Following an incubation period of 10 days, stereoisomeric selectivity is detectable with the glucose pair by mass spectrometry in extracts made from soil at 0.5 g/ml, in extracts made from JSC Mars-1 at 2.5 g/ml, and in cell suspensions down to 1.0 x 10(7) cells/ml. For the tetrasaccharide pair, stereoisomeric selectivity was detected in extracts made from 0.5 g/ml or more of common soil but not in JSC Mars-1 simulant. The effective sensitivity in extracts was 2.5 x 10(7) cells/ml or better for the glucose pair and 5.0 x 10(8) cells/ml or better for the tetrasaccharide pair. The sensitivity of the glucose pair was such that it could detect life in samples that would be found to be devoid of organic matter by the GCMS system carried by the Viking landers. The results demonstrate the utility of the approach in the search for biological activity on Mars. However, sensitivity is a function of the enantiomer pair used, and this might also be different for hypothetical martian organisms. Therefore, it will be necessary to characterize additional stereoisomeric pairs and, ultimately, to include several in a single test environment.

Exploration Class missions to Mars will require precautions against potential contamination by any native microorganisms that may be incidentally pathogenic to humans. While the results of NASA's Viking biology experiments of the 1970s have been generally interpreted as inconclusive for surface organisms, and attributed to active but nonbiological chemistries, the possibility of native surface life has never been ruled out completely. It is possible that, prior to the first human landing on Mars, robotic craft and sample return missions will provide enough data to know with certainty whether future human landing sites harbor extant life forms. If native life were found to exist, it would be problematic to determine whether any of its species might present a medical danger to astronauts. Therefore, it will become necessary to assess empirically the risk that the planet contains pathogens based on terrestrial examples of pathogenicity and to take a reasonably cautious approach to biohazard protection. A survey of terrestrial pathogens was conducted with special emphasis on those whose evolution has not depended on the presence of animal hosts. The history of the development and implementation of Apollo anti-contamination protocol and recommendations of the National Research Council's Space Studies Board regarding Mars were reviewed. Organisms can emerge in Nature in the absence of indigenous animal hosts and both infectious and non-infectious human pathogens are therefore theoretically possible on Mars. Although remote, the prospect of Martian surface life, together with the existence of a diversity of routes by which pathogenicity has emerged on Earth, suggests that the probability of human pathogens on Mars, while low, is not zero. Still, since the discovery and study of Martian life can have long-term benefits for humanity, the risk that Martian life might include pathogens should not be an obstacle to human exploration. As a precaution, it is recommended that EVA (extravehicular activity) suits be decontaminated when astronauts enter surface habitats upon returning from field activity and that biosafety protocols approximating laboratory BSL 2 be developed for astronauts working in laboratories on the Martian surface. Quarantine of astronauts and Martian materials arriving on Earth should also be part of a human mission to Mars, and this and the surface biosafety program should be integral to human expeditions from the earliest stages of the mission planning.

Microorganisms are unavoidable in space environments and their presence has, at times, been a source of problems. Concerns about disease during human space missions are particularly important considering the significant changes the immune system incurs during spaceflight and the history of microbial contamination aboard the Mir space station. Additionally, these contaminants may have adverse effects on instrumentation and life-support systems. A sensitive, highly specific system to detect, characterize, and monitor these microbial populations is essential. Herein we describe a monitoring approach that uses 16S rRNA targeted molecular beacons to successfully detect several specific bacterial groupings. This methodology will greatly simplify in-flight monitoring by minimizing sample handling and processing. We also address and provide solutions to target accessibility problems encountered in hybridizations that target 16S rRNA.

Exploration Class missions to Mars will require precautions against potential contamination by any native microorganisms that may be incidentally pathogenic to humans. While the results of NASA's Viking biology experiments of 1976 have been generally interpreted as inconclusive for surface organisms, the possibility of native surface life has never been ruled out and more recent studies suggest that the case for biological interpretation of the Viking Labeled Release data may now be stronger than it was when the experiments were originally conducted. It is possible that, prior to the first human landing on Mars, robotic craft and sample return missions will provide enough data to know with certainty whether or not future human landing sites harbor extant life forms. However, if native life is confirmed, it will be problematic to determine whether any of its species may present a medical risk to astronauts. Therefore, it will become necessary to assess empirically the risk that the planet contains pathogens based on terrestrial examples of pathogenicity and to take a reasonably cautious approach to bio-hazard protection. A survey of terrestrial pathogens was conducted with special emphasis on those pathogens whose evolution has not depended on the presence of animal hosts. The history of the development and implementation of Apollo anticontamination protocol and recent recommendations of the NRC Space Studies Board regarding Mars were reviewed. Organisms can emerge in nature in the absence of indigenous animal hosts and both infectious and non-infectious human pathogens are theoretically possible on Mars. The prospect of Martian surface life, together with the existence of a diversity of routes by which pathogenicity has emerged on Earth, suggests that the possibility of human pathogens on Mars, while low, is not zero. Since the discovery and study of Martian life can have long-term benefits for humanity, the risk that Martian life might include pathogens should not be an obstacle to human exploration. As a precaution, however, it is recommended that EVA suits be decontaminated when astronauts enter surface habitats when returning from field activity and that biosafety protocol approximating laboratory BSL 2 be developed for astronauts working in laboratories on the Martian surface. Quarantine of astronauts and Martian materials arriving on Earth should also be part of a human Mars mission and this and the surface biosafety program should be integral to human expeditions from the earliest stages of the mission planning.

We have developed a SQUID-based technique. to probe membrane-bound proteins, such as cation transporters.' The method involves excitation of a biological sample with a sinusoidal electric field (0-5 V/cm, 10-300 Hz), detection, with a SQUID magnetometer, of the magnetic field produced by ac currents in the tissue or cell suspension, and calculation of the Fast Fourier Transform (FFT) of the measured response to obtain a spectrum. We find that the harmonic response of budding yeast cells (S. cerevisiae, 10(8) cells/ml) is sensitive to sodium metavanadate, an inhibitor of a proton pump known as H+ -ATPase, glucose, a substrate of H+ -ATPase, and gramicidin, a membrane depolarizer. SQUID-based field and frequency dependent harmonic response measurements are also made of a live earthworm (Lumbricus terrestris). Use of the SQUID significantly reduces spurious harmonics and distortion of the excitation field by the measurement electrodes.

adrenocortical steroids due to a dysfunctional hypothalamic-pituitaryadrenocortical (HPA) axis.METHODS: We evaluated the pre-(VB2) and post-prostatic massage (VB3) urine levels of several novel biomarkers of dysfunctional HPA axis activity.VB2 and VB3 samples were collected from 24 men with a diagnosis of CP/CPPS (median age, 48 years) and 30 age-matched asymptomatic controls.Patients and controls underwent standardized clinical evaluation and were required to complete the National Institutes Chronic Prostatitis Symptom Index (NIH-CPSI).The following biomarkers were measured in VB2 and VB3 using ELISA kits: corticotropin releasing hormone (CRH), neuropeptide Y (NPY), both obtained from Phoenix Pharmaceuticals, Belmont, CA), dehydroepiandrosterone (DHEA) and epidermal growth factor (EGF) from R & D Systems, Minneapolis, MN.Galanin levels were measured using RIA (Phoenix Pharmaceuticals, Belmont, CA).Modified allostatic load score was calculated based on a review of the neuroscientific literature.RESULTS: Allostatic load scores were significantly higher in patients with CPPS as compared with controls (P<0.001).Furthermore, PPMU NPY and galanin levels in CPPS patients were significantly lower as compared to those in controls.The PPMU of CRH and DHEA were significantly higher as compared with controls (P<0.001).CONCLUSIONS: Our results show evidence of allostatic overload in CPPS patients as compared with controls and provide exciting insights into possible therapeutic avenues for the management of CPPS based on allostatic scores.

Developments over the past decade have given new credibility to the idea that Earth's biosphere could have arisen from an extraterrestrial seed. New research indicates that microorganisms could have survived a journey from Mars to Earth.

We report on the linear and nonlinear dielectric properties of budding yeast (S. cerevisiae) cells, one strain of which has been genetically modified to express prestin. This motor protein plays a crucial role in the large electromotility exhibited by the outer hair cells of mammalian inner ears. Live cell suspensions exhibit enormous dielectric responses, which can be used to probe metabolic activity, membrane potential, and other properties. The aims of this study are: (1) to compare the dielectric responses of organisms expressing prestin from those of control specimens, and (2) ultimately to further develop dielectric response as a tool to study live cells, proteins, and lipids.

Abstract—Researchers at the University of Houston and NASA-JSC seek to develop high frequency methods, such as broadband and nar- rowband (resonant) dielectric spectroscopy tech- niques, for the investigation of Martian soil sim- ulants and live cell suspensions. Such methods hold great potential for use in high frequency characterization of the magnetite crystals found inthe Martian meteorite Allan Hills 84001. Magnetite compounds found in the meteorite consist of a common inorganic rock and a bio- genic product, which can be formed by a variety oforganisms. It is clear that characterization and comparison of earth and Martian magneto- fossils can provide significant information relat- edto the evolution and history of Mars. In this initial study, we have developed high frequency dielectric spectroscopy probes and conducted test measurements of dielectric properties of live

The possibility that the positive outcome of the Viking Labeled Release Experiment (LR) had resulted from the presence of extant Martian microorganisms in samples examined on Mars was dismissed based largely on the failure of the Viking Gas Chromatograph-Mass Spectrometer (GCMS) to demonstrate the presence of organic mole-cules. More recent findings suggesting that the Viking GCMS would have missed such molecules if present necessitates a re-evaluation of the Viking LR data as well as a continued search for organic material and life at the Martian surface. In addition to advanced mass spectrometers to look for organic signatures of biological processes, future lander missions may use biological techniques, such as immunoassay, to directly detect bio-organic molecules. Meanwhile, several decades in advance of any planned sample return missions, the examination of Mars samples already present on Earth in the form of the SNC meteorites indicates that organic matter has existed in the Martian upper crust. It is concluded that a biological interpretation of the LR on Mars cannot be dismissed and should now be considered at least as plausible as a non-biological interpretation until more complete studies of the Martian sur-face are carried out.

To demonstrate the feasibility of two promising technologies, we have applied Enzyme-Linked Immunosorbent Assay (ELISA) as well as probes that target the 16S rRNA molecule to search for life in terrestrial soil samples, known to contain numerous life forms. Additional information is contained in the original extended abstract.