Slowed lymphocyte rolling along high endothelial venules (HEV) is initiated by the L selectin: PNAd interaction, as an essential first step in a cascade of reactions leading to extravasation of lymphocytes across HEV and into the lymphoid tissues. CHST4, a golgi resident carbohydrate sulfotransferase, has been described as an HEV restricted sulfotransferase in part responsible for the sulfation step required for L selectin: PNAd interaction. The MECA79 antibody which recognizes sulfated PNAd provides a useful tool for monitoring sulfotransferase activity. The presence of both CHST4 and the MECA79 sulfated epitope have been described in HEV as well as lymphoid aggregates and tertiary lymphoid tissues at the sites of chronic inflammation. For the studies described, we investigated the expression of CHST4 with real time PCR, In Situ Hybridization (ISH), Immunohistochemistry (IHC) as well as MECA79 IHC. Expression patterns were examined in an extensive collection of normal human tissue panels as well as diseased tissue collected from several inflammatory conditions. Resulting data, in addition to confirming an HEV and lymphoid aggregate restricted expression profile also revealed expression of the gene in epithelial and ductal epithelial cells in a small set of diverse organ tissues. The functional consequences of such expression and the co-localized MECA79 epitope expression are currently unknown.