Insufficient biologically available nitrogen (N) for yeast is a persistent issue facing cidermakers, whose apple juice base usually does not provide adequate nutrition for a complete fermentation. Cidermakers often supplement their juice with additional yeast assimilable nitrogen (YAN) in the cellar to aid fermentation. The development of biologically available N in apple juice is not well understood. In this study, juice samples from ‘Crimson Crisp®’ apples were taken at several sampling dates in the 2016, 2017, and 2018 growing seasons and analyzed for YAN using formol titration and high-performance liquid chromatography. It was observed that while the total YAN concentration in these apples drops from the period shortly after fruit set to the end of summer, YAN remains stable from several weeks before harvest until the date of harvest. The total YAN did not change after a 6-week postharvest storage period. By contrast, the individual amino acid components of YAN do change during this period. This experiment shows that foliar urea sprays in ‘Crimson Crisp®’ produce an increase in organic N in the juice, mostly in the form of asparagine. Increased organic N impacts yeast growth and sensory characteristics of cider and may be seen as desirable by cider producers.
Twenty-eight apple cultivars were selected for their potential for hard cider production in Ontario; their juice characteristics were measured in 2017 and 2018, beginning two years after planting in 2015. After being harvested and pressed, each juice sample underwent analyses to determine soluble solids concentration (SSC), titratable acidity (TA), pH, yeast assimilable nitrogen (YAN), and polyphenolic concentration. Soluble solids concentration ranged from 10.6 °Brix in Brown’s Apple to 18.3 °Brix in Ashmead’s Kernel. Titratable acidity ranged from 31 as mg malic acid per 100 mL juice in Sweet Alford to 191 as mg malic acid per 100 mL juice in Bramley’s Seedling. The pH ranged from 2.88 in Breakwell to 4.76 in Sweet Alford. Yeast assimilable nitrogen concentration ranged from 60 mg YAN·L−1juice in Medaille d’Or to 256 mg YAN·L−1juice in Bulmer’s Norman. Polyphenols in juice ranged from 131 μg gallic acid equivalents (gae)·mL−1juice in Tolman Sweet to 1042 μg gae·mL−1juice in Stoke Red. Firmness ranged from 6.3 kg in Yarlington Mill to 11.7 kg in GoldRush. The relationships between these variables were also analyzed, showing a connection between acidity and juicing efficiency as well as a relationship between polyphenol concentration and fruit weight. Exploratory analyses indicated that juice attributes can be used to distinguish between cultivars and their origins. Cider producers can use these data to determine what to expect in juice from these cultivars.
Continued interest in the commercial production of cider in Ontario has revealed that there is a lack of information available to apple producers and cidermakers on the juice attributes of culinary dessert apple for making cider and how they may vary from orchard to orchard. A two-year study was conducted to characterize the juice characteristics of 18 common culinary dessert apple cultivars grown at several locations in Ontario. Juice was assayed for soluble solids concentration, pH, titratable acidity (TA), juice extraction efficiency, yeast assimilable nitrogen (YAN), polyphenols, and soluble solids to TA ratio. Significant differences among cultivars were observed for all parameters, which varied by orchard and year, except juice extraction efficiency. These data underly the importance of assaying juice every growing season prior to fermentation. Cidermakers should focus on juice characteristics from culinary apples that are more difficult to alter (with amendments), and use juice with lower pH, higher TA and polyphenols, and moderate to higher YAN to optimize cider quality and flavour. Overall, this study will inform cidermakers of which culinary apple cultivars might be preferable for fermentation as well as how they might be blended to optimize cider quality. Notwithstanding annual and orchard variation, cultivars that meet these criteria include Cortland, Crimson Crisp, Honeycrisp, Jonagold, McIntosh, and Northern Spy. If making cider from Ambrosia, Crispin, Empire, Fuji, Gala, Golden Delicious, or Idared, cidermakers should expect to blend the juice from these cultivars to create a more fully balanced juice prior to fermentation.