From the stems of Alstonia scholaris, a new secoiridoid glucoside, named alstonoside 1, together with two isoflavone apioglucosides, formononetin 7-O-beta-D-apifuranosyl-(1 -> 6)-beta-D-glucopyranoside 2 and biochanin A 7-O-beta-D-apiofuranosyl- 1 -> 6)-beta-D-glucopyranoside 3 arc isolated and their structures are elucidated on the basis of detailed 1D and 2D-NMR and mass spectral data. Alstonoside features the presence of a ring opened monocyclic iridoid glucoside with the presence of an O-benzoyl moiety. This study constitutes the first report of a new secoiridoid glucoside and two isoflavone apioglucosides from this plant.

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