Residues,source and chiral signatures of three classical organochlorine pesticides(OCPs)-dichlorodiphenyltrichloroethanes(DDTs),chlordane(CHLs),and hexachlorocyclohexanes(HCHs) in five species of seafoods from Zhoushan fishing ground were studied.The concentrations of ∑HCHs,∑CHLs and ∑DDTs in seafoods ranged from 6.00×10-9～431.02×10-9,0.17×10-9～3.94×10-9 and 0.57×10-9～2.81×10-9,respectively.Moreover,the accumulation ability of all organochlorine pesticides(OCPs) in shellfish was much stronger than that in shrimp and bombay duck.The composition profiles indicated that the residues of HCHs and DDTs in these seafoods mainly originated from the history usage of technical OCPs,while CHLs may have new inputs.The enantiomeric fractions(EFs) of α-HCH,trans-chlorodane(TC),cis-chlorodane(CC),o,p'-DDT,and o,p'-DDD were determined.The results showed that all chiral OCPs but o,p'-DDD have obviously enantiomeric excess.In addition,OCPs residues were far below the edible hygienic criteria in China.The estimated daily intake of OCPs from the seafoods we tested were also below the acceptable daily intake(ADI) recommend by FAO/WHO,indicating a little health risk in seafood consumption for local residents.