ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew substrate for fluorometric determination of oxidative enzymesGeorge G. Guilbault, David N. Kramer, and Ethel B. HackleyCite this: Anal. Chem. 1967, 39, 2, 271Publication Date (Print):February 1, 1967Publication History Published online1 May 2002Published inissue 1 February 1967https://pubs.acs.org/doi/10.1021/ac60246a029https://doi.org/10.1021/ac60246a029research-articleACS PublicationsRequest reuse permissionsArticle Views199Altmetric-Citations87LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

A fluorometric method is described for the assay of the enzyme hyaluronidase and of Cu(II), Fe(II), and cyanide ions which inhibit the enzymic activity. The method for hyaluronidase is based upon the hydrolysis of the nonfluorescent indoxyl acetate by the enzyme to give the highly fluorescent indigo white (λex = 395 mμ, λem = 470 mμ). Metal ions, such as Fe++ and Cu++, and CN−, which inhibit the enzyme, can be determined by recording their effect on the enzymic activity. By this procedure, from 0.0010 to 0.0330 μg/ml of hyaluronidase, 0.10 to 4.0 μg/ml of CN−, 0.20 to 12 μg/ml of Fe++, and 0.20 to 6 μg/ml of Cu++ could be determined with standard deviations of 1.8% for hyaluronidase and 2.3% for the inhibitors. Evidence is offered to demonstrate hyaluronidase, and not esterase, activity.

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRapid, sensitive kinetic method for detection and determination of phenolic compoundsGeorge G. Guilbault, David N. Kramer, and Ethel B. HackleyCite this: Anal. Chem. 1966, 38, 13, 1897–1899Publication Date (Print):December 1, 1966Publication History Published online1 May 2002Published inissue 1 December 1966https://pubs.acs.org/doi/10.1021/ac50155a055https://doi.org/10.1021/ac50155a055research-articleACS PublicationsRequest reuse permissionsArticle Views64Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts