Background. This work compares the osteoblastic behaviour of a bone marrow (BM) aspirate and a prepared BM concentrate of nucleated cells associated with a glass reinforced hydroxyapatite composite (GRHC) in a microporous pellet formulation. Methods. BM aspirate (30 mL) was collected during 3 orthopedic surgical procedures, and a concentration system was used to achieve 3 rapid preparations of a concentrate of nucleated cells (3 mL) from the BM aspirates. The BM aspirates (53% cell viability; 2.7×106 nucleated cell/mL) and the BM concentrates (76% cell viability; 2×107 nucleated cell/mL) were cultured over glass reinforced hydroxyapatite pellets, at the same volume/mass ratio, for 30 days. Cultures performed in standard tissue culture plates were used as control. Results. The colonized BM concentrate/material constructs exhibited a representative osteoblastic proliferation/differentiation pathway, evidenced by a high alkaline phosphatase (ALP) activity, expression of collagen type 1, ALP, BMP-2, M-CSF, RANKL, and OPG, and formation of a calcium phosphate mineralized matrix. A clear improved behaviour was noticed compared to the BM aspirate/material constructs. Conclusions. The results suggest the benefit of using an autologous BM concentrate/material construct in the clinical setting, in bone regeneration applications.

OBJECTIVE:To conduct a bibliographic review of the infrequent fractures of the carpal scaphoid in pediatric cases based on a clinical case. The diagnosis is usually a difficult one and treatment should be stringent in order to minimize the chance of delayed healing or pseudoarthrosis.MATERIAL AND METHODS:Ten year-old child with a carpal scaphoid fracture visible in the plain films. The patient did not comply with treatment and removed the cast at three weeks. Despite this healing occurred without any clinical sequelae.DISCUSSION:This case is especially infrequent given the excellent result obtained despite the fact that the immobilization period was considerably shorter than the minimum recommended.

Mostrar mediante un caso clínico que las fracturas del hueso ganchoso son muy poco frecuentes, mucho menos aún las que afectan al cuerpo del mismo sin fracturas asociadas de otros huesos del carpo. Debido a su infrecuencia y a la inespecificidad de sus manifestaciones clínicas, suelen ser de difícil diagnóstico. Presentamos el caso de un varón de 24 años, que sufrió fractura conminuta aislada del cuerpo del hueso ganchoso. Fue tratado de forma conservadora, obteniéndose una recuperación ad integrum sin déficit funcional. Es importante considerar este tipo de fracturas ante un traumatismo directo sobre la mano. Es recomendable la realización de una tomografia computerizada (TC) para caracterizar correctamente la fractura y valorar las posibles lesiones asociadas. La decisión terapéutica depende del grado de conminución de la fractura, de su estabilidad y de la afectación articular. To demonstrate, through a clinical case, that fractures of the hamate bone are very infrequent and even more so those that affect its body without there being fractures of the other associated carpal bones. Due to its infrequency and to the non-specificity of its clinical manifestations, they are usually difficult to diagnose. We present a case of a 24 year old male who suffered an isolated comminuted fracture of the body of the hamate bone. It was treated conservatively and full recovery without any functional deficit was obtained. It is important to consider this type of fracture when confronted with a direct traumatism to the hand. Performing computed tomography (CT) is recommended in order to correctly identify the fracture and assess any possible associated lesions. The therapeutic decision depends on the degree of comminution of the fracture, its stability and any joint involvement.

To demonstrate, through a clinical case, that fractures of the hamate bone are very infrequent and even more so those that affect its body without there being fractures of the other associated carpal bones. Due to its infrequency and to the nonspecificity of its clinical manifestations, they are usually difficult to diagnose. We present a case of a 24 year old male who suffered an isolated comminuted fracture of the body of the hamate bone. It was treated conservatively and full recovery without any functional deficit was obtained. It is important to consider this type of fracture when confronted with a direct traumatism to the hand. Performing computed tomography (CT) is recommended in order to correctly identify the fracture and assess any possible associated lesions. The therapeutic decision depends on the degree of comminution of the fracture, its stability and any joint involvement. Mostrar mediante un caso clínico que las fracturas del hueso ganchoso son muy poco frecuentes, mucho menos aún las que afectan al cuerpo del mismo sin fracturas asociadas de otros huesos del carpo. Debido a su infrecuencia y a la inespecificidad de sus manifestaciones clínicas, suelen ser de difícil diagnóstico. Presentamos el caso de un varón de 24 años, que sufrió fractura conminuta aislada del cuerpo del hueso ganchoso. Fue tratado de forma conservadora, obteniéndose una recuperación ad integrum sin déficit funcional. Es importante considerar este tipo de fracturas ante un traumatismo directo sobre la mano. Es recomendable la realización de una tomografía computerizada (TC) para caracterizar correctamente la fractura y valorar las posibles lesiones asociadas. La decisión terapéutica depende del grado de conminución de la fractura, de su estabilidad y de la afectación articular.