We compared migration, proliferation, growth curve, confluency and differentiation into adipogenic, osteogenic and chondrogenic cell lineages of mesenchymal stem cells derived from adipose tissue cultured in scratched and nonscratched cell culture flasks. Mesenchymal stem cells were isolated from rat adipose tissue using a nonenzymatic method. We investigated two groups. For the control group, minced adipose tissue was implanted conventionally onto the surface of standard plastic cell culture flasks. For the experimental group, the tissues were cultured in flasks with a scratched surface. We found that scratched flasks promoted cell migration, proliferation and confluency. Our findings suggest that scratched flasks may be used to ensure rapid, practical and safe isolation of adipose tissue-derived stem cells.

In this study, histologic and electromyographic (EMG) evaluation of neuroregenerative effect of stromal vascular fraction (SVF) following periferal nerve anastomosis was aimed.Totally, 31 Wistar Albino, male rats (weighing about 300 g) were studied, and these rats were grouped according to surgical techniques performed on the right sciatic nerve: group I (GRI) micro suture (n=7), group II (GRII) micro suture + SVF (n=7), group III (GRIII) fibrin glue (n=7) and group IV (GRIV) fibrin glue + SVF (n=7).Fat tissue was taken from 3 rats to prepare SVF, and SVF was produced by non-enzymatic method.The left sciatic nerve of all rats was evaluated for EMG as control.Under general anesthesia, after transversal incision of the sciatic nerve, microsurgical epineural repair technique was performed with 10/0 nonabsorbable suture.EMG examinations were performed in terms of conduction rate, amplitude, distal latency and spontaneous muscle activity at 0th day and postoperative (PO) 1st and 8 th weeks.EMG results between and within the groups were statistically evaluated by one-way analysis of variance.Rats in all groups were sacrificed by decapitation at PO 8 th week and histological examinations of the sciatic nerves were performed following preparation of the neural tissues.EMG examination results showed the highest nerve conduction in GRI, the highest amplitude in GRIII, normal latency in GRII and longer distal latency in GRIV at PO 8 th week.Amplitude and conduction velocity increased gradually in all groups.In needle EMG, the best muscle membrane stabilization was achieved in GRII and GRIV at PO 8 th week.Statistically, the values of amplitude, distal latency, conduction velocity, and spontaneous muscle activity were found to be at normal levels at PO 8 th week in all groups (P>0.05).In the histological results, although fibroconnective tissue reactions in the anastomosis area had similar scores in GRII and GRIV, maximum fibroconnective tissue reaction and the best axonal regeneration was seen in GRI and GRIII, respectively.In addition, GRI and GRII had the most inflammatory cells accumulation in the suture region, and less inflammatory cells were seen in the anastomosis area of GRIII and GRIV.As a conclusion, fibrin glue presents good electrophysiological and histological results; however, it is clear that local SVF usage on the nerve anastomosis area can be a good choice to decrease fibroconnective tissue reaction and inflammation.

Mezenkimal kök hücreler (MKH), hayvanlar ve insanlarda yüksek oranda bulunan ve terapötik etkinliğe sahip farklılaşmamış hücrelerdir. Bu çalışmada, yağ dokudan izole edilen pasaj 3 hücrelerinde, MKH özelliklerinin tanımlanması, Ki-67 antikorunun immunolokalizasyonu, hücre gelişim analizi ile proliferasyonun değerlendirilmesi amaçlandı. Pasaj 3 hücreleri adiposit, osteoblast ve kondroblastlara farklılaştırılarak Oil Red O, Alizarin Red ve Alcian Blue teknikleri ile boyandı. MKH karakterizasyonu; kök hücre yüzey işaretleyicilerinden CD 90 ve CD 105 ile pozitif; CD 45 ve CD 11b ile negatif ekspresyonu tanımlandı. Sonuç olarak çalışmada; yağ doku kökenli pasaj 3 hücrelerde; immünofenotipik karakterizasyonun, osteojenik, kondrojenik ve adipojenik yönde farklılaşmanın, Ki-67 immunpozitif hücrelerde, PDT bulgularında optimal hücresel hemostazisin korunduğu ve terapötik açıdan sağlıklı mezenkimal kök hücre proliferasyonunun gerçekleştiği gösterilmiştir.

The therapeutic potential of the adipose tissue is also supported by a source of mesenchymal stem cells (MSCs), endothelial progenitor cells, mast cells, T- lymphocyte, B lymphocyte, and adipose-resident macrophages with repairing and regenerative ability. The purpose of this study was to compare adipose tissue derived mesenchymal stem cells (ADSCs) and stromal vascular fraction cells (SVF cells) in terms of the usage of non-enzymatic isolation, detection of cell adhesion, fibroblastoid cell formation, properties of cell population, cell culture duration until 3rd passages under in-vitro culture condition. We used 3 months old, 4 male Sprague Dawley rats (mean of live weight about 250 g) to examined the variation of the cell population, cell properties, phases of cellular formation, cell culturing time, subculturing duration, differences in confluency between SVF cells and ADSCs. In in-vitro expansion, ADSCs displayed higher rate of adhesiveness, homogenous cell population, faster proliferation and formation of fibroblast like cells compared to SVF cells. As a result, we showed that ADSCs have better adhesive ability, higher proliferative capacity in all of the 3 passages and require shorter time to reach confluency compared with SVF cells in vitro. These findings may contribute to future studies that deal with isolation and selection of stem cells from various tissues, as well as design clinical trials based on ADSCs and SVF cells.

In this study, histologic and electromyographic (EMG) evaluation of neuroregenerative effect of stromal vascular fraction (SVF) following periferal nerve anastomosis was aimed. Totally, 31 Wistar Albino, male rats (weighing about 300 g) were studied, and these rats were grouped according to surgical techniques performed on the right sciatic nerve: group I (GRI) micro suture (n=7), group II (GRII) micro suture + SVF (n=7), group III (GRIII) fibrin glue (n=7) and group IV (GRIV) fibrin glue + SVF (n=7). Fat tissue was taken from 3 rats to prepare SVF, and SVF was produced by non-enzymatic method. The left sciatic nerve of all rats was evaluated for EMG as control. Under general anesthesia, after transversal incision of the sciatic nerve, microsurgical epineural repair technique was performed with 10/0 nonabsorbable suture. EMG examinations were performed in terms of conduction rate, amplitude, distal latency and spontaneous muscle activity at 0th day and postoperative (PO) 1st and 8th weeks. EMG results between and within the groups were statistically evaluated by one-way analysis of variance. Rats in all groups were sacrificed by decapitation at PO 8th week and histological examinations of the sciatic nerves were performed following preparation of the neural tissues. EMG examination results showed the highest nerve conduction in GRI, the highest amplitude in GRIII, normal latency in GRII and longer distal latency in GRIV at PO 8th week. Amplitude and conduction velocity increased gradually in all groups. In needle EMG, the best muscle membrane stabilization was achieved in GRII and GRIV at PO 8th week. Statistically, the values of amplitude, distal latency, conduction velocity, and spontaneous muscle activity were found to be at normal levels at PO 8th week in all groups (P>0.05). In the histological results, although fibroconnective tissue reactions in the anastomosis area had similar scores in GRII and GRIV, maximum fibroconnective tissue reaction and the best axonal regeneration was seen in GRI and GRIII, respectively. In addition, GRI and GRII had the most inflammatory cells accumulation in the suture region, and less inflammatory cells were seen in the anastomosis area of GRIII and GRIV. As a conclusion, fibrin glue presents good electrophysiological and histological results; however, it is clear that local SVF usage on the nerve anastomosis area can be a good choice to decrease fibroconnective tissue reaction and inflammation.

Nesfatin-1 is a multifunctional neuropeptide having crucial autonomic roles. It is well known that nesfatin-1 collaborates with other central neuromodulatory systems, such as central corticotropin-releasing hormone, melanocortin, oxytocin, and cholinergic systems to show its autonomic effects. Central arachidonic acid cascade plays an important role to provide the homeostasis by exhibiting similar autonomic effects to nesfatin-1. Based on these similarities, the current study was designed to show the effects of intracerebroventricularly (ICV) injected nesfatin-1 on the hypothalamic arachidonic acid (AA) cascade. Immunochemistry and western blot approaches demonstrated that ICV administration of nesfatin-1 provokes an increase in the hypothalamic cyclooxygenase (COX) -1, -2 and lipoxygenase (LOX) protein expression. Moreover, the microdialysis study demonstrated that centrally injected nesfatin-1 increased the posterior hypothalamic extracellular AA products. In conclusion, these findings report that while nesfatin-1 is generating its autonomic effects, it also might be using central prostaglandins and leukotrienes by activating central COX and LOX pathways.

Stem cells are known as undifferentiated cells that are capable of divisions and self-renewal over an extended period. Adipose tissue-derived mesenchymal stem cells (ADSCs) are known to differentiate into many different organ and tissue cells. The purpose of this study was to characterize adipose tissue-derived mesenchymal stem cells in detail based on the expression status of certain surface markers and differentiation stages for clinical and laboratory applications. The migrating phase and mitotic property of the cells from adipose tissue were assessed under a microscope. Cell counting was performed during the first, second, and third passages. The cells were differentiated into osteogenic, chondrogenic, and adipogenic lineages. Stem cells were characterized by immunostaining of CD90, CD105, CD11b, and CD45 surface markers. ADSC growth kinetics were determined at the first, second, and third passages. In conclusion, ADSCs were reported in detail here based on surface markers and differentiation potentials, growth curves, and population doubling times during the first, second, and third passages.