The mobile bag technique (MBT) was used in two ileorectostomized and two intact pigs with duodenal cannulae, in order to estimate the precaecal and total crude protein digestibility and amino acid (lysine, methionine, cystine, threonine, tryptophan) absorption of ten single feedstuffs (field bean, lupin, rapeseed meal, fish meal, barley, wheat) and three diets. For each feed 36 bags were applied per animal. After a peptic predigestion in vitro, application of the bags through the duodenal cannula into the intestine and their passage through the gut, the residues remaining in the bags were pooled to collective samples and just as the original feed material analysed on dry matter, nitrogen and amino acids. The directly determined MBT-digestibilities (BV) and the values obtained by regressive calculations (BVb) have been compared with apparent digestibilities (sV) conducted from conventional digestion experiments. 74 per cent of all BV data was higher than the corresponding sV values. Similarities of BV with sV showed in 58 per cent, those of BVb with sV only in 17 per cent. In all cases differences of > +/- 4 digestibility units showed. Checking additivity of diet-BV by means of calculations from the BV of their single ingredients did not generally lead to the same results as the directly estimated diet-BV; in some cases the results were contrary. Therefore the use of MBT for mixed feed diets has to be further investigated. For single feedstuffs the MBT can be used successfully as a rapid and simple technique when regressive calculations are employed.

Fur die Durchfuhrung der mobilen Beuteltechnik (MBT), die zur Ermittlung der prazakalen und Gesamt-Rohproteinverdaulichkeit und Aminosaurenresorption (Lysin, Methionin, Zystin, Threonin, Tryptophan) bei 10 Einzelfuttermitteln (Ackerbohne, Lupine, Rapsextraktionsschrot, Fischmehl, Gerste, Weizen) und 3 Rationen zur Anwendung kam, wurden jeweils 2 weibliche ileorektostomierte sowie intakte Schweine mit einfachen Duodenalkanulen benutzt. Je Versuchsfutter und Tier kamen 36 Polyamidbeutel zum Einsatz. Nach peptischer Vorverdauung in vitro, Applikation durch die Duodenalkanule und Passage durch den Darmtrakt wurden die im Beutel verbliebenen Ruckstande fur jedes Tier und Futtermittel zu einer Sammelprobe vereinigt und diese wie das Ausgangsmaterial auf Trockensubstanz, Stickstoff und Aminosauren analysiert. Die direkt ermittelten Verdaulichkeiten (BV) und uber regressive Berechnung erhaltenen Werte (BVb) wurden mit den in konventionellen Verdauungsversuchen bestimmten scheinbaren Verdaulichkeiten (sV)...

In faeces and ileal digesta samples of 31 intact (INT) as well as 73 surgically differently prepared pigs bacterial fractionations and 2.6-diaminopimelic acid (DAP) estimations were carried out in order to calculate the bacterial N proportion in faeces N and digesta N after feeding various diets. Because of a high individual variability and the analytical variation width of the DAP/N-ratios no distinct influences of the fed diets could be found. The average DAP/N-values in the faeces (0.224) of INT pigs ranged in the same magnitude as in the digesta (0.0272) of ileorectostomized (IRA) pigs with open colon descendens (IRAo), where a digesta backflow is possible. Distinct lower DAP/N-ratios (0.0125 resp. 0.0043), however, were found in the digesta of pigs with ileo-caecal cannulae (IZB) or IRA pigs with closed colon (IRAg). On the base of various premises (N of the "bacterial fraction" C is only bacterial N; the DAP found in fraction A originates from intestinal bacteria adhering to feed particles) conducted calculations of the bacterial N proportions (in per cent of total N) led to the following data: Faeces of INT pigs: 43.0 ... 68.2 vs. 69.6 ... 89.0; digesta of operated pigs (except protein free diet) IRAo: 22.3 ... 57.0 vs. 46.2 ... 73.8; IZB: 17.0 ... 35.7 vs. 25.2 ... 53.6; IRAg (only 3 pigs): 23.6 vs. 24.2. The proportion of bacterial N in the digesta N of protein free fed IRAo pigs was 22.0 vs. 22.6%.

(1984). Anwendung der HCl-unloslichen Asche als Indikator zur Bestimmung der Nahrstoffeinschlieslich Aminosaurenverdaulichkeit am Ende des Dunndarms und des Gesamtverdauungstraktes Beim Schwein. Archiv fur Tierernaehrung: Vol. 34, No. 12, pp. 817-831.