Cette invention concerne des conjugues comprenant une biomolecule liee a un marqueur qui ont une activite biologique et sont utiles dans toute une variete d'applications biologiques. Par exemple, des conjugues polymerase-nanoparticule comprenant une polymerase liee a une nanoparticule sont decrits, ledit conjugue ayant une activite polymerase. Ces conjugues peuvent demontrer une agregation reduite et des stœchiometries ameliorees, le rapport moyen biomolecule:nanoparticule etant voisin ou egal a 1:1. Des procedes ameliores pour preparer ces conjugues, et des procedes et des systemes pour utiliser ces conjugues dans des applications biologiques telles que l'incorporation de nucleotides, l'allongement d'amorces et le sequencage de molecules individuelles sont egalement decrits.

La presente invention concerne des nanocristaux ayant un noyau a base d'indium et leurs procedes de fabrication et leurs utilisations pour construire des nanocristaux a noyau central. Ces nanocristaux a noyau central sont tres stables et fournissent des rendements quantiques plus eleves que ceux des nanocristaux connus de composition similaire, et ils apportent des avantages speciaux a certaines applications du fait de leur petite taille.

L'invention concerne des procedes de modification/fonctionnalisation, y compris reticulation, de ligands dans l'enrobage de surface d'une nanoparticule ou dans les molecules d'autres enrobages d'une nanoparticule. Ces procedes reposent sur l'utilisation de reactions d'ajout de radicaux pour ajouter un groupe reactif a un ligand/molecule d'une nanoparticule. Des exemples comprennent l'utilisation d'une benzophenone fonctionnalisee qui est liee ou reticulee a un ligand de l'enrobage de surface d'un nanocristal par ajout de radicaux photochimiquement amorces.

We have investigated rarely observed Se-77 J-couplings (spin-spin couplings) in the mixed-metal face-capped octahedral clusters [Re5OsSe8(CN)(6)](3-) and [Re4Os2Se8(CN)(6)](2-) at natural abundance. To the best of our knowledge, these are the first observations of Se-Se spin-spin interactions between mu(3)-Se sites, important for stereochemical assignments in hexarhenium analogues, Chevrel phase materials, and similar cluster materials. NMR techniques such as COSY, INADEQUATE, and 2D J-resolved spectroscopy have been used in conjunction to study these interactions. The two isomers (cis and trans) of [Re4Os2Se8(CN)(6)](2-) were distinguishable, and selective isotopic labeling of [Re5OsSe8(CN)(6)](3-) with (CN)-C-13 ligands enabled resonances to be assigned by observing the (2)J (Se-M-C) couplings. For [Re5OsSe8(CN)(6)](3-), two different (2)J (Se-M-Se) couplings were measurable on a single cluster, and these are related to one another through spin-spin interactions across a face diagonal or along an edge of the cube of inner selenium ligands. A rigorous analysis based on combinatorial math has been invoked to assign the couplings on the basis of the probability of multiple-spin interactions. The face diagonal association is found to result in a J-coupling interaction larger in magnitude than that from coupling along an edge of the cubeinformation critical for making stereochemical assignments of selenium sites.

We have investigated rarely observed 77Se J-couplings (spin-spin couplings) in the mixed-metal face-capped octahedral clusters [Re5OsSe8(CN)6]3- and [Re4Os2Se8(CN)6]2- at natural abundance. To the best of our knowledge, these are the first observations of Se-Se spin-spin interactions between mu3-Se sites, important for stereochemical assignments in hexarhenium analogues, Chevrel phase materials, and similar cluster materials. NMR techniques such as COSY, INADEQUATE, and 2D J-resolved spectroscopy have been used in conjunction to study these interactions. The two isomers (cis and trans) of [Re4Os2Se8(CN)6]2- were distinguishable, and selective isotopic labeling of [Re5OsSe8(CN)6]3- with 13CN ligands enabled resonances to be assigned by observing the 2J (Se-M-C) couplings. For [Re5OsSe8(CN)6]3-, two different 2J (Se-M-Se) couplings were measurable on a single cluster, and these are related to one another through spin-spin interactions across a face diagonal or along an edge of the cube of inner selenium ligands. A rigorous analysis based on combinatorial math has been invoked to assign the couplings on the basis of the probability of multiple-spin interactions. The face diagonal association is found to result in a J-coupling interaction larger in magnitude than that from coupling along an edge of the cube-information critical for making stereochemical assignments of selenium sites.