Supplementary Table 1 from Class III β-Tubulin Expression Predicts Prostate Tumor Aggressiveness and Patient Response to Docetaxel-Based Chemotherapy

Abstract Expression of class III β-tubulin (βIII-tubulin) correlates with tumor progression and resistance to taxane-based therapies for several human malignancies, but its use as a biomarker of tumor behavior in prostate cancer (PCa) remains largely unexplored. Here, we describe βIII-tubulin immunohistochemical staining patterns of prostate tumors obtained from a broad spectrum of PCa patients, some of whom subsequently received docetaxel therapy for castration-resistant PCa (CRPC). Elevated βIII-tubulin expression was significantly associated with tumor aggressiveness in PCa patients with presumed localized disease, as it was found to be an independent marker of biochemical recurrence after treatment. Additionally, βIII-tubulin expression in tumor cells was an independent predictor of lower overall survival for patients receiving docetaxel-based chemotherapy for CRPC. Manipulation of βIII-tubulin expression in human PCa cell lines using a human βIII-tubulin expression vector or βIII-tubulin small interfering RNA altered cell survival in response to docetaxel treatment in a manner that supports a role for βIII-tubulin expression as a mediator of PCa cell resistance to docetaxel therapy. Our findings suggest a role for βIII-tubulin as candidate theranostic biomarker to predict the response to docetaxel-based chemotherapy as well as to target for treatment of docetaxel-resistant CRPC. Cancer Res; 70(22); 9253–64. ©2010 AACR.

Downloa ression of class III β-tubulin (βIII-tubulin) correlates with tumor progression and resistance to taxanetherapies for several human malignancies, but its use as a biomarker of tumor behavior in prostate r (PCa) remains largely unexplored. Here, we describe βIII-tubulin immunohistochemical staining ns of prostate tumors obtained from a broad spectrum of PCa patients, some of whom subsequently ed docetaxel therapy for castration-resistant PCa (CRPC). Elevated βIII-tubulin expression was signifiassociated with tumor aggressiveness in PCa patients with presumed localized disease, as it was found n independent marker of biochemical recurrence after treatment. Additionally, βIII-tubulin expression or cells was an independent predictor of lower overall survival for patients receiving docetaxel-based therapy for CRPC. Manipulation of βIII-tubulin expression in human PCa cell lines using a human βIIIn expression vector or βIII-tubulin small interfering RNA altered cell survival in response to docetaxel ent in a manner that supports a role for βIII-tubulin expression as a mediator of PCa cell resistance to xel therapy. Our findings suggest a role for βIII-tubulin as candidate theranostic biomarker to predict the response to docetaxel-based chemotherapy as well as to target for treatment of docetaxel-resistant CRPC. Cancer Res; 70(22); 9253–64. ©2010 AACR.