Arnica montana is a clonal, self-incompatible herb of economic and intrinsic ecological value which is declining in large parts of its range. With the employment of microsatellite markers, we characterized the population structure and distribution of genetic diversity of 40 populations of A. montana sampled throughout Europe. We detected a clear geographical pattern of isolation and strong population structure, indicating limited gene flow. We also observed a negative latitudinal gradient in genetic diversity. Such patterns can be explained by paleo-historical colonization routes following the last glacial maximum, with regions characterized by higher genetic diversity corresponding to former glacial refugia. We recommend the implementation of conservation measures such as assisted gene flow in the populations characterized by low genetic diversity under consideration of the observed population structure. For the populations where high levels of genetic diversity are still retained, we recommend the maintenance of suitable habitat conditions. Our study emphasises the importance of conducting large-scale population genetic studies that consider postglacial recolonization history when planning active conservation measures such as assisted gene flow.

8 Habitat loss and degradation due to human-induced landscape alterations are considered to 9 be a major threat to biodiversity. The decline of biodiversity may occur with a time delay 10 leading to a so called extinction debt. Therefore, determining extinction risks and 11 conservation status is not always straightforward. Several life history traits might play a role 12 for the accumulation of an extinction debt. Thus, perennial plant species capable of 13 vegetative propagation might be able to persist temporarily in degraded habitats even 14 though sexual and evolutionary processes are effectively halted. 15 We studied Cnidium dubium, which occurs in scattered patches along river corridors in 16 Central Europe and is critically endangered in Germany. It is a perennial species which is able 17 to propagate clonally. Our aims were to reconstruct demographic processes regarding clonal 18 propagation and gene flow along 400 km of river stretch and with respect to the position in 19 the flooplain, i.e. before or behind dykes. We also wanted to determine whether there is 20 evidence for an extinction debt in C. dubium and to use our insights for conservation 21

Habitat loss and degradation due to human-induced landscape alterations are considered to be a major threat to biodiversity. The decline of biodiversity may occur with a time delay leading to a so called extinction debt. Therefore, determining extinction risks and conservation status is not always straightforward. Several life history traits might play a role for the accumulation of an extinction debt. Thus, perennial plant species capable of vegetative propagation might be able to persist temporarily in degraded habitats even though sexual and evolutionary processes are effectively halted.We studied Cnidium dubium , which occurs in scattered patches along river corridors in Central Europe and is critically endangered in Germany. It is a perennial species which is able to propagate clonally. Our aims were to reconstruct demographic processes regarding clonal propagation and gene flow along 400 km of river stretch and with respect to the position in the flooplain, i.e. before or behind dykes. We also wanted to determine whether there is evidence for an extinction debt in C. dubium and to use our insights for conservation recommendations.For this, we used nuclear microsatellites and maternally inherited chloroplast DNA markers and applied a systematic grid based sampling strategy for small scale geographic structures.We observed a high level of clonal propagation. In 935 analysed plants we observed only 121 different genotypes and of 50 studied patches of C. dubium the majority (31 patches) consisted of one single genotype each. Patch size and position were correlated with clonal diversity. Large patches and patches behind dykes exhibited higher clonal diversity. There was no evidence for a large scale genetic substructuring of the study area and no differences in overall genetic diversity between upstream and downstream patches as well as between patches before and behind the dykes. High levels of heterozygosity and a high number of 18 chloroplast DNA haplotypes togetherwith a slightly elevated inbreeding coefficient (Fis) point toward a high level of ancestral polymorphism in an out of equilibrium population due to high levels of clonal propagation and low levels of gene flow and recombination. Therefore, we assume that an extinction debt is present in C. dubium. As a management strategy, we propose to transplant ramets between multiple patches to increase the number of mating partners and therefore restore sexual reproduction.### Competing Interest StatementThe authors have declared no competing interest.

The performance of seedlings is crucial for the survival and persistence of plant populations. Although drought frequently occurs in floodplains and can cause seedling mortality, studies on the effects of drought on seedlings of floodplain grasslands are scarce. We tested the hypotheses that drought reduces aboveground biomass, total biomass, plant height, number of leaves, leaf area and specific leaf area (SLA), and increases root biomass and root-mass fraction (RMF) and that seedlings from species of wet floodplain grasslands are more affected by drought than species of dry grasslands. In a greenhouse study, we exposed seedlings of three confamilial pairs of species (Pimpinella saxifraga, Selinum carvifolia, Veronica teucrium, Veronica maritima, Sanguisorba minor, Sanguisorba officinalis) to increasing drought treatments. Within each plant family, one species is characteristic of wet and one of dry floodplain grasslands, confamilial in order to avoid phylogenetic bias of the results. In accordance with our hypotheses, drought conditions reduced aboveground biomass, total biomass, plant height, number of leaves and leaf area. Contrary to our hypotheses, drought conditions increased SLA and decreased root biomass and RMF of seedlings. Beyond the effects of the families, the results were species-specific (V. maritima being the most sensitive species) and habitat-specific. Species indicative of wet floodplain grasslands appear to be more sensitive to drought than species indicative of dry grasslands. Because of species- and habitat-specific responses to reduced water availability, future drought periods due to climate change may severely affect some species from dry and wet habitats, while others may be unaffected.

Including population genetic aspects into the selection of planting material within the framework of conservation and restoration measures is of vital importance for the long-term persistence of populations. This is especially true facing climate change since genetic diversity and the spread of potentially beneficial alleles are important for the adaptability of populations. Therefore, knowledge about genetic variability within and between populations is a critical aspect when determining provenance regions. In our study, we investigated the population genetic structure of a widespread, insect-pollinated and mainly bird-dispersed shrub species, Frangula alnus, on the basis of seven microsatellites and two chloroplast DNA markers throughout Germany. The aim was to determine the spatial, temporal and ecological processes genetically structuring populations to critically revise existing provenance regions. Therefore, we conducted analyses on different spatial scales (country-wide, regional and local) using the two different marker sets in addition to environmental variables. We detected distinct patterns on all spatial scales which indicated influences of historic recolonization processes, regional differences of seed dispersal across the landscape, as well as small-scale spatial genetic structures attributable to local dispersal processes. No relation of underlying environmental gradients such as temperature or precipitation and genetic patterns was found. We conclude that different aspects of historic and more recent gene flow shape population genetic structures and that a thorough analysis on a variety of spatial, temporal and environmental scales is necessary to appropriately select planting material for conservation and restoration measures. Correspondingly, management advice regarding provenance delineations will be provided.

Abstract Salicaceae are typical invaders of riparian ecosystems throughout the world and they have the potential to change much of the invaded habitats. Along Patagonian streams, riparian softwood forests composed of non-native Salicaceae are increasing in abundance, area and tree species diversity. Especially in the last decades, dense floodplain forests dominated by invasive willows and poplars and additionally Russian olive and tamarisk spread almost explosively e.g. along the Río Negro in northern Patagonia. This study focuses on the identification of ecological niches and niche overlaps of the native and invasive woody species in order to assess the impact on the native Salix humboldtiana Willd. Data on species (presence/ absence) and explanatory variables were gathered in the field using a grid-based, stratified-randomized sampling design. Different environmental variables were then related to species occurrence in different life stages (adult, juvenile, seedling) using habitat distribution models. In the final models, flood duration, the amount of gravel and the location (upper or middle river valley) were included to describe the probability of occurrence of native and invasive taxa. For all life stages, a strong niche overlap could be observed for S. humboldtiana and the invasive taxa with no remaining exclusive habitats indicating a potential threat to the native willow. The study contributes to a better understanding of Salicaceae invasion and its consequences for riparian ecosystems.

Climate change is expected to alter temperature and precipitation patterns and thereby river discharges. As floodplain vegetation types in Central Europe are mainly structured by the local hydrologic conditions of the river, alterations in the flow regime should lead to changes in the structure of floodplain communities, too. However, much uncertainty about potential hydrologic changes exists due to the variability in modeled discharges by different global and regional climate models. Aim of our study was to assess potential changes and their uncertainty in the future habitat availability of plant species representing characteristic floodplain vegetation types along the Rhine River. Therefore, habitat distribution models for 29 different species were calculated relating species distribution to local hydrologic conditions. Habitat changes were assessed by comparing future habitat availability of five different climate change projections with reference state conditions for four stretches along the Rhine for two different future periods. Models revealed that besides different water level parameters also water level variability was decisive for habitat distribution patterns. Relative habitat changes showed large variation for the different species between and within vegetation types, as well as for the different river stretches and future periods. However, intersection of the five projections displayed large overlap regarding future habitat availability for many of the species. Moreover, a tendency of the different vegetation types to occur on higher elevated sites was found. Our study emphasizes the necessity to consider the variability of different climate change projections to reasonably assess potential changes in habitat availability of floodplain vegetation and provides a tool to identify species groups and river sections vulnerable to climate change induced hydrologic alterations. (C) 2015 Elsevier B.V. All rights reserved.

Background Floodplain meadows along rivers are semi-natural habitats and depend on regular land use. When used non-intensively, they offer suitable habitats for many plant species including rare ones. Floodplains are hydrologically dynamic ecosystems with both periods of flooding and of dry conditions. In German floodplains, dry periods may increase due to reduced summer precipitation as projected by climate change scenarios. Against this background, the question arises, how the forage quantity and quality of these meadows might change in future. Methods We report results of two field trials that investigated effects of experimentally reduced summer precipitation on hay quantity and quality of floodplain meadows at the Rhine River (2011-2012) and at two Elbe tributaries (2009-2011). We measured annual yield, the amount of hay biomass, and contents of crude protein, crude fibre, energy, fructan, nitrogen, phosphorus, and potassium. Results The annual yield decreased under precipitation reduction at the Rhine River. This was due to reduced productivity in the second cut hay at the Rhine River in which, interestingly, the contents of nitrogen and crude protein increased. The first cut at the Rhine River was unaffected by the treatments. At the Elbe tributaries, the annual yield and the hay quantity and quality of both cuts were only marginally affected by the treatments. Conclusion We conclude that the yield of floodplain meadows may become less reliable in future since the annual yield decreased under precipitation reduction at the Rhine River. However, the first and agriculturally more important cut was almost unaffected by the precipitation reduction, which is probably due to sufficient soil moisture from winter/spring. As long as future water levels of the rivers will not decrease during spring, at least the use of the hay from the first cut of floodplain meadows appears reliable under climate change.

Abstract Floodplain meadow ecosystems are characterized by high water level fluctuations and highly variable soil water potentials. Additionally, climate change scenarios indicate an increasing risk for summer drought along the northern Upper Rhine and the Middle Elbe River, Germany. While adult plants often persist even after strong changes in water availability, early life phases, such as seed germination and seedling establishment, might be more vulnerable. Therefore we tested whether reduced soil water potentials will affect the germination of meadow species and whether the response varies between (1) forbs indicative of wet and dry habitats and (2) seeds originating from sites along the rivers Elbe and Rhine. We exposed seeds of 20 floodplain meadow species with different moisture requirements from five plant families to a water potential gradient ranging from 0 to − 1.5 MPa. While across species germination percentage and synchrony decreased, germination time increased at reduced water potentials. Germination of the species indicative of dry habitats decreased more strongly, was slower and less synchronous at reduced water potentials than that of species indicative of wet habitats. Seeds from sites along the rivers Elbe and Rhine did not differ in their germination characteristics. We propose that species of wet sites follow an all-or-nothing-strategy with fast and synchronous germination to maximize competitive advantages, betting on a high probability of moist conditions for establishment (optimists). In contrast, species from dry sites appear to follow a bet-hedging strategy with a moisture-sensing mechanism for unsuitable conditions (pessimists), resulting in a slower and less synchronous germination.

•Stretching over a broad climatic gradient, floodplain plants may serve as indicators for climate change effects.•Only species composition of wet meadows changed along the Middle Elbe River.•River corridor plants were evenly distributed along the gradient.•River corridor plants appear relatively robust against direct climatic changes.•Total assemblages of wet meadows might be relatively vulnerable to climate change.

Hydrological and land use changes can affect species in human altered landscapes. Typically the impacts of hydrological and land use changes are examined separately, with hydrological determinants used to explain the distribution of species in water dependent and aquatic habitats and land use factors used to examine terrestrial species. However, given the connectedness of aquatic and terrestrial habitats, stressors originating in one domain may be important in the other. To explore the importance of integrating both hydrological and land use factors, we tested the relative contribution of hydrological factors and land use context as determinants of the dominant riparian tree species, Eucalyptus camaldulensis Dehn. throughout wetlands of the Condamine catchment, southern Queensland, Australia. The occurrence of E. camaldulensis was modelled against hydrological and land use factors using generalized linear models (GLMs) and validated using internal bootstrapping procedures. Validated models which included both hydrological (distance from weir, wetland–river connectivity and groundwater depth) and land use factors (agricultural land cover and grazing intensity) performed better than those developed using only hydrological factors. The study results highlight the importance of an integrated perspective which considers both hydrological and land use factors in order to understand occurrence patterns of riparian and floodplain tree species in a range of settings. This approach could be especially important when assessing changes to hydrology and land use which may be triggered by climatic changes.

AuVeg (GIVD ID EU-DE-028) is a database containing phytosociological relevés of German floodplain vegetation including key environmental variables such as hydrological and soil data.It provides essential information to further understand ecological interactions in floodplain habitats.Furthermore, it provides baseline data for the development of habitat models of plant species and vegetation types.Such models will be used to assess the effect of climate change-induced alterations of the riverine flow regime on plant habitats.They are also used in practice supporting the environmental impact assessment in case of riverine construction or maintenance works.

Throughout Europe the demands for improved flood protection on the one hand and the requirements to maintain and enhance floodplain forests on the other are perceived as conflicting goals in river-basin management, revealing the urgent need for strategies to combine both issues. We developed an interdisciplinary approach for floodplain-forest restoration identifying sites suitable for reforestations from both an ecological and hydraulic point of view. In the ecological module, habitat-distribution models are developed providing information on ecologically suitable sites. In the hydraulic module, a two-dimensional hydrodynamic-numerical model (2D-HN model) delivers the requested hydraulic information. The output of the two models is intersected. Subsequently, in an iterative procedure, the potential of plantings without exceeding critical water levels can be identified by hydraulic evaluation using the 2D-HN-model. The approach is exemplified using two reforestation scenarios at the Elbe River, Germany, showing considerable potential for softwood forest establishment without negative hydraulic effects.The approach reported here provides a solution for a severe conflict in river-basin management that hampers the reestablishment of the strongly threatened floodplain forests in Europe. Alternative measures to enhance floodplain-forest regeneration feasible under certain preconditions are discussed in the context of the current state of European large rivers.

In Central Europe, floodplain fragmentation by dykes and river regulation by dams has led to a fundamental loss of riparian softwood forests and dramatically restricted natural regeneration of the Salicaceae species involved. As a consequence, reforestation is one of the widely-used measures to maintain this valuable vegetation type. Knowledge about the genetic population structure of existing stands of willows and poplars is necessary to preserve their genetic potential in the long term. In this study, we investigated clonal and genetic diversity patterns of Salix viminalis for 23 stands, taking into account young and old age classes and locations in front of the dykes in the active floodplain and behind the dykes in the inactive floodplain along the Elbe River, Germany. Microsatellites were used to assess effects of stand and floodplain fragmentation and to evaluate the extent of clonal structures. We detected considerable numbers of multi-ramet-clones almost exclusively in the active floodplain with old stands exhibiting the largest number of these clones. Individual clones mostly covered distances of less than 10m, but single long distance dispersal events of vegetative propagules were also detected. Genetic diversity patterns revealed one continuous population although a certain small-scale spatial genetic structure was identified. Our study suggests that riparian softwood forest species have efficient dispersal strategies which allow the population to survive altered floodplain conditions from a genetic point of view. Management advice is given for selecting genetically suitable planting material for restoration purposes.

While several studies on regeneration in Salicaceae have focused on seedling recruitment, little is known about factors controlling their vegetative reproduction. In two greenhouse experiments, we studied the response of floodplain willows ( Salix fragilis , S. viminalis , S. triandra ) to competition with Poa trivialis , and to shoot and root removal when planted as vegetative cuttings. In the first experiment, growth performance variables were analysed in relation to full competition, shoot competition, root competition and control, taking into account two different water levels. After 9 weeks, shoots were removed and the resprouting capacity of the bare cuttings was recorded. In the second experiment, the cutting performance of the three floodplain and an additional two fen willow species ( S. cinerea , S. aurita ) was compared when grown in three different soil compositions and with two different water levels. After 9 weeks, shoot and root biomass was removed and the bare cuttings were replanted to test their ability to resprout. Cutting performance and secondary resprouting were negatively affected by full and shoot competition while root competition had no or weak effects. The floodplain species performed better than the fen species in all soil types and water levels. Secondary resprouting capacity was also higher in the floodplain species, which showed an additional strong positive response to the previous waterlogging treatment. The results contribute to understanding of the vegetative regeneration ecology of floodplain willows, and suggest that the use of vegetative plantings in restoration plantings could be an effective strategy for recovering floodplain forests.

Understanding mechanisms to predict changes in plant and animal communities is a key challenge in ecology. The need to transfer knowledge gained from single species to a more generalized approach has led to the development of categorization systems where species' similarities in life strategies and traits are classified into ecological groups (EGs) like functional groups/types or guilds. While approaches in plant ecology undergo a steady improvement and refinement of methodologies, progression in animal ecology is lagging behind. With this review, we aim to initiate a further development of functional classification systems in animal ecology, comparable to recent developments in plant ecology. We here (i) give an overview of terms and definitions of EGs in animal ecology, (ii) discuss existing classification systems, methods and application areas of EGs (focusing on terrestrial vertebrates), and (iii) provide a "roadmap towards an animal functional type approach" for improving the application of EGs and classifications in animal ecology. We found that an animal functional type approach requires: (i) the identification of core traits describing species' dependency on their habitat and life history traits, (ii) an optimization of trait selection by clustering traits into hierarchies, (iii) the assessment of "soft traits" as substitute for hardly measurable traits, e.g. body size for dispersal ability, and (iv) testing of delineated groups for validation including experiments.

Willows of the Salix alba–Salix fragilis complex, native to western Eurasia, represent typical invaders of floodplain ecosystems worldwide. Introduced to South America by European settlers probably at the end of the nineteenth century, their distribution has increased significantly along the rivers in Northern Patagonia. This case study carried out mainly in the area around Lake Nahuel Huapi aims to analyze clonal structures and their spatial distribution using molecular markers as well as to relate the observed patterns to settlement history and life history traits of this species complex. Leaf material from 171 trees was collected along selected river floodplains in Northern Patagonia and genotypes were determined at six microsatellite loci. Including 62 reference samples of the S. alba–S. fragilis complex from German rivers, Probability of Identity (P ID) was calculated and a Principal Coordinate Analysis (PCoA) conducted. From the altogether thirteen different genotypes detected, one dominant genotype (female) formed monoclonal stands along most of the studied river stretches. The maximum linear distance between the most remote ramets of this clone was 790 km. Evidence arose that the colonizing process so far is exclusively based on vegetative propagation in the focal study area and is obviously attributable to the pronounced brittleness of the hybrid parent S. fragilis. However, outside this area the occurrence of male trees and a diversity of genotypes indicate that evolutionary processes by recombination are involved within the willow complex. Therefore, an increase in genotypes can be assumed when male individuals and therefore sexual reproduction would appear in the area around Lake Nuhuel Huapi. This could be a crucial point for the long-term invasion success of the taxa when climatic and other environmental conditions will change in Southern Argentina.

Question: What are the hydrological requirements for the successful reforestation of riparian Salix communities? Do differences in site conditions between various life stages of woody vegetation types need to be considered? Do interactions between hydrological factors influence distribution patterns?Location: Mid-reaches of the Elbe River, Germany.Methods: Young and old life stages of two different riparian Salix communities were surveyed in 1067 plots of 400 m(2) to determine hydrological growth conditions using habitat distribution models. Models were extrapolated in a Geographic Information System to quantify the extent of potential stands available for reforestation measures.Results: Average water level and water level fluctuations were related to different vegetation types as well as to the age classes of the vegetation types. Differences in hydrological niches of young and old vegetation of the two vegetation types (tree community versus shrub community) could be identified. Moreover, the shrub vegetation was influenced by the interaction of average water level and fluctuations. Comparison of the distribution of current vegetation and suitable habitat revealed that considerable areas of the floodplain were suitable for the reforestation of Salix woodland communities.Conclusions: Hydrological variables explain the distribution patterns of riparian Salix communities in the active floodplain when different life stages, vegetation types and interaction of variables are included in the analyses. This information can be used to recommend suitable sites for Salix plantings in riparian landscapes.

Riparian and floodplain ecosystems in production landscapes are considerably degraded and under continued pressure from surrounding land use. However, little is known about how remnant ecosystems respond to land use and hydrological factors in small non-riverine wetlands. River red gum (Eucalyptus camaldulensis) is a dominant tree species within these scattered remnants, which provides critical ecological functions for the remaining biodiversity. In this study, we investigated how different life stages of E. camaldulensis responded to land use and hydrological variables in the Condamine catchment of south east Queensland. We used logistic regression to develop models for different life stages of E. camaldulensis in two regions with differing land use intensity histories. Broad regional differences and land use practices at smaller scales best explained differences in E. camaldulensis occurrence for younger life stages, while hydrology (groundwater and connectivity to rivers) and land use practices (dryland agriculture and grazing) best explained differences in older life stages. The results indicate that different factors are important in determining the establishment and persistence of E. camaldulensis and that land use practices at the regional scale are key factors in determining the establishment and potential future persistence of E. camaldulensis in floodplain wetlands.