The impact of ileostomy on intestinal microflora and bacterial translocation has been investigated in 21 adults rats. Animals were divided in three groups: Group I, control, group II, sham underwent laparotomy alone, and Group IIII, laparotomy and terminal ileostomy. 7 days later, animals were killed and different intestinal segments, and organs were harvested for bacteriological analysis. The number of colony forming units for intestinal samples was expressed per intestinal content. All results showed a normal distribution and were expressed as mean standard error. Unpaired Student's t-test was used. Ileostomy induced dysmicrobims, the microflora imbalance was particularly observed in ileum and caecum. Bacterial translocation to kidneys, spleen and liver occurred in control group. Laparotomy and particularly ileostomy significantly increased the bacterial translocation. Ileostomy induced a dysmicrobism by disruption of the microbial ecology balance of the gastro-intestinal tract and significantly increased bacterial translocation.

Journal of Pediatric Gastroenterology and NutritionVolume 39, Issue S1 p. S264-S264 ABSTRACTS: Poster Session Abstracts P0546 ACTION OF AMOXYCILLIN/CLAVULANATE AGAINST INTESTINAL MICROFLORA: EVALUATION BY SIMULATOR OF HUMAN INTESTINAL MICROBIAL ECOSYSTEM: VITROSIM A. Tir touil Meddah, A. Tir touil Meddah Département de Pédiatrie-Médecine Périnatale et Réanimation Pédiatrique, Faculté de Médecine, CHU Nord, Université de Picardie Jules verne, Amiens, FranceSearch for more papers by this authorL. Leke, L. Leke Département de Pédiatrie-Médecine Périnatale et Réanimation Pédiatrique, Faculté de Médecine, CHU Nord, Université de Picardie Jules verne, Amiens, FranceSearch for more papers by this authorS. Hurtel, S. Hurtel Laboratoire de Pharmacologie, CHU Sud, CVG, Amiens, FranceSearch for more papers by this authorS. Delanaud, S. Delanaud Laboratoire ETP-APC, Faculté de médecine, CVG, Amiens, FranceSearch for more papers by this authorT. Stadler, T. Stadler Centre de Valorisation des Glucides et Produits naturels, CVG, Amiens, FranceSearch for more papers by this authorJ. Dupas, J. Dupas Département de gastroentérologie, CHU Nord, Amiens, FranceSearch for more papers by this authorJ. Canarelli, J. Canarelli Département de Pédiatrie-Médecine Périnatale et Réanimation Pédiatrique, Faculté de Médecine, CHU Nord, Amiens, FranceSearch for more papers by this authorF. Eb, F. Eb Laboratoire de Bactériologie et d’Hygienne, CHU Nord, Amiens, FranceSearch for more papers by this author A. Tir touil Meddah, A. Tir touil Meddah Département de Pédiatrie-Médecine Périnatale et Réanimation Pédiatrique, Faculté de Médecine, CHU Nord, Université de Picardie Jules verne, Amiens, FranceSearch for more papers by this authorL. Leke, L. Leke Département de Pédiatrie-Médecine Périnatale et Réanimation Pédiatrique, Faculté de Médecine, CHU Nord, Université de Picardie Jules verne, Amiens, FranceSearch for more papers by this authorS. Hurtel, S. Hurtel Laboratoire de Pharmacologie, CHU Sud, CVG, Amiens, FranceSearch for more papers by this authorS. Delanaud, S. Delanaud Laboratoire ETP-APC, Faculté de médecine, CVG, Amiens, FranceSearch for more papers by this authorT. Stadler, T. Stadler Centre de Valorisation des Glucides et Produits naturels, CVG, Amiens, FranceSearch for more papers by this authorJ. Dupas, J. Dupas Département de gastroentérologie, CHU Nord, Amiens, FranceSearch for more papers by this authorJ. Canarelli, J. Canarelli Département de Pédiatrie-Médecine Périnatale et Réanimation Pédiatrique, Faculté de Médecine, CHU Nord, Amiens, FranceSearch for more papers by this authorF. Eb, F. Eb Laboratoire de Bactériologie et d’Hygienne, CHU Nord, Amiens, FranceSearch for more papers by this author First published: 01 June 2004 https://doi.org/10.1002/j.1536-4801.2004.tb12976.x Submitted by: [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume39, IssueS1June 2004Pages S264-S264 RelatedInformation

We investigated the action of amoxicillin-clavulanic acid (Augmentin)(R) an in vitro dynamic model of human gastrointestinal microbial ecosystem. The composition of the fecal microflora from three volunteers was inoculated in the system and compared with data obtained from human patients. 750mg/93.25mgx2/day of the amoxycillin/clavunate were added daily for 3 days. The effect on the microbial ecology was evaluated before administration, during treatment at day 3, and 5 days after the administration was stopped. The treatment induced a decrease in bifidobacteria and lactobacilli and increase in enterobacteria and Clostridium sp in all reactors (p < 0.05). The number tended to restore to their original levels after the administration was stopped. Bacteroides sp was not affected by the treatment in the ileum and descending colon, but significantly increased in transverse and descending colon (p < 0.05). The count did not found the original level after the treatment. Total aerobe and anaerobe bacteria were slightly influenced but remained relatively unchanging over time during the treatment. In conclusion, Augmentin (R) clearly altered the microbial community. These findings were similar and confirmed by in vivo studies (clinical situations).