Animal GeneticsVolume 30, Issue 1 p. 77-78 Extreme fragment lengths differences of the microsatellite in the expressed MHC-DRB gene of Merinoland sheep I. Griesinger, I. Griesinger Department of Animal Breeding and Biotechnology, University of Hohenheim, 70599 Stuttgart, GermanySearch for more papers by this authorF. Prüser, F. Prüser Department of Animal Breeding and Biotechnology, University of Hohenheim, 70599 Stuttgart, GermanySearch for more papers by this authorK. Siemienski, K. Siemienski Department of Animal Breeding and Biotechnology, University of Hohenheim, 70599 Stuttgart, GermanySearch for more papers by this authorH. Geldermann, H. Geldermann Department of Animal Breeding and Biotechnology, University of Hohenheim, 70599 Stuttgart, GermanySearch for more papers by this author I. Griesinger, I. Griesinger Department of Animal Breeding and Biotechnology, University of Hohenheim, 70599 Stuttgart, GermanySearch for more papers by this authorF. Prüser, F. Prüser Department of Animal Breeding and Biotechnology, University of Hohenheim, 70599 Stuttgart, GermanySearch for more papers by this authorK. Siemienski, K. Siemienski Department of Animal Breeding and Biotechnology, University of Hohenheim, 70599 Stuttgart, GermanySearch for more papers by this authorH. Geldermann, H. Geldermann Department of Animal Breeding and Biotechnology, University of Hohenheim, 70599 Stuttgart, GermanySearch for more papers by this author First published: 24 December 2001 https://doi.org/10.1046/j.1365-2052.1999.00323-17.xCitations: 3About ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article.Citing Literature Volume30, Issue1February 1999Pages 77-78 RelatedInformation

The estimation of DNA substitution rates requires calibration with the geological events which caused the separation of populations. We used the disintegration of the landbridges between Morocco and Gibraltar as well as the Balearics and Spain at the end of the Messinian event (5.3 myr) in order to calibrate the DNA sequence data of the NADH‐dehydrogenase subunit 1 (ND1) in West Mediterranean Carabus species. The rates of molecular evolution we found are much lower than those reported in the literature and also vary from species to species. Therefore, in insects the calculation of divergence periods using a constant 2% substitution rate per myr is questionable.

Three sympatric forms have been found within the morphospecies Ceroglossus chilensis Esch. through karyotype analysis and characterization of repetitive DNA families. The male chromosome number of these forms is 2n = 28 + XY (form A), 2n = 40 + XY (form B), and 2n = 38 + trivalent (form C). Restriction enzyme analysis of total genomic DNA of form B using EcoRI produces several conspicuous bands corresponding to monomers and dimers of two repetitive DNA families, of about 575 bp and 200 bp respectively; form C has, after total digestion, only one band of 625 bp corresponding to the monomer of a different repetitive DNA family; and form A has no conspicuous band after this treatment. In situ hybridization experiments show that repetitive DNAs are specific for each form and no crossed hybridization is observed. The results suggest that these Ceroglossus forms are actually a complex of sibling species evolving as separate evolutionary and phylogenetic units, whose characterization at the morphological, ecological and geographical levels should be the subject of further investigation.

The larva of Ceroglossus chilensis Eschscholtz is described in all instars and compared with the larva of C. suturalis Fabricius. Typical characteristics of the genus Ceroglossus are head with neck constricted, plesiomorph pattern of chaetotaxy compared with that of Carabini larvae and urogomphi very long and segmented.