This paper presents a wake-up circuit for battery-assisted passive RFID applications. The circuit is composed by a differential envelope detector and a comparator with a systematic offset. The differential RF signal envelope detector and the comparator are designed to increase the wake-up circuit sensitivity and to have low power consumption, improving battery lifetime. The wake-up circuit was designed and implemented in standard CMOS 0.18 um process as part of an UHF RFID battery-assisted passive system, achieving a sensitivity of -31 dBm and consuming only 150 nA.