Environmental stressors induce specific physiological responses that can be measured in the blood, notably by morphological changes in lymphocytes. Tobacco being the best-known stress in terms of its impact on health, we studied the physiological properties of peripheral blood lymphocytes in a population of 33 healthy non-smokers and smokers. Proteasome amount, mitochondria energy levels, changes in membrane properties and cell and nuclear size were analyzed to obtain 28 parameters from two fluorescence-based techniques: flow cytometry and cell imaging. The results showed that none of the parameters alone identified gender and smoking status, but that statistical analysis of these parameters, whether or not combined with a third set of data, hematological data, can. Statistical analysis of selected parameters clearly discriminates between male and female samples, as well as smokers and non-smokers. Effects of tobacco smoke pollutants are more pronounced in female smokers than in other groups.

The aim of this study was to test the effect of face cooling with cold water (1.2 ± 0.7°C) vs. face cooling with neutral water (28.0 ± 3.0°C) during high-intensity swimming training on both the core temperature (Tco) and thermal perceptions in internationally ranked long-distance swimmers (5 men’s and 3 women’s) during 2 randomized swimming sessions. After a standardized warm-up of 1,200 m, the athletes performed a standardized training session that consisted of 2,000 m (5 × 400 m; start every 5’15”) at a best velocity then 600 m of aerobic work. Heart rate (HR) was continuously monitored during 5 × 400 m, whereas Tco, thermal comfort (TC), and thermal sensation (TS) were measured before and after each 400 m. Before and after each 400 m, the swimmers were asked to flow 200 mL of cold water (1.2°C) or neutral (22°C) water packaged in standardized bottles on their face. The swimmers were asked don’t drink during exercise. The velocity was significantly different between cold water and neutral water (p < 0.004 – 71.58 m.min–1 ± 2.32 and 70.52 m.min–1 ± 1.73, respectively). The Tco was increased by ±0.5°C at race pace, under both face cooling conditions with no significant difference. No significant changes were noted in mean HR (i.e., 115 ± 9 and 114 ± 15 bpm for NW and CW, respectively). TC was higher with Cold Cooling than Neutral Cooling and TS was lower with Cold cooling compared with Neutral cooling. The changes in perceptual parameters caused by face cooling with cold water reflect the psychological impact on the physical parameters. The mean velocity was less important with face cooling whereas the heat rate and Tco were the same in the both conditions. The mechanism leading to these results seems to involve brain integration of signals from physiological and psychological sources.

The practice of physical activity in a variable climate during the same competition is becoming more and more common due to climate change and increasingly frequent climate disturbances. The main aim of this pilot study was to understand the impact of cold ambient temperature on performance factors during a professional cycling race. Six professional athletes (age = 27 ± 2.7 years; height = 180.86 ± 5.81 cm; weight = 74.09 ± 9.11 kg; % fat mass = 8.01 ± 2.47%; maximum aerobic power (MAP) = 473 ± 26.28 W, undertook ~20 h training each week at the time of the study) participated in the Tour de la Provence under cold environmental conditions (the ambient temperature was 15.6 ± 1.4 °C with a relative humidity of 41 ± 8.5% and the normalized ambient temperature (Tawc) was 7.77 ± 2.04 °C). Body core temperature (Tco) was measured with an ingestible capsule. Heart rate (HR), power, speed, cadence and the elevation gradient were read from the cyclists’ onboard performance monitors. The interaction (multivariate analysis of variance) of the Tawc and the elevation gradient has a significant impact (F(1.5) = 32.2; p < 0.001) on the variables (cadence, power, velocity, core temperature, heart rate) and on each individual. Thus, this pilot study shows that in cold environmental conditions, the athlete’s performance was limited by weather parameters (ambient temperature associated with air velocity) and race characteristics. The interaction of Tawc and elevation gradient significantly influences thermal (Tco), physiological (HR) and performance (power, speed and cadence) factors. Therefore, it is advisable to develop warm-up, hydration and clothing strategies for competitive cycling under cold ambient conditions and to acclimatize to the cold by training in the same conditions to those that may be encountered in competition.

ABSTRACT Olympic distance cross-country cycling (XCO) is a discipline subject to wide performance variability due to uncontrollable environmental factors such as altitude, ambient temperature and/or humidity. This study therefore aimed to investigate the impact of environmental factors on XCO performance in under-23 and elite female and male categories. Individual data were collected from Continental Cup, World Cup, World Championship, and Olympics Games for U23 and elite female and male categories from 2009 to 2018. Factors included were race time (range: 55–157 min), average speed (range: 7.6–32.2 km/h), distance (range: 15.2–48.4 km), altitude (range: 50–2680 m), ambient temperature (range 7–41°C), relative and absolute humidity (range: 8–97% and 2.4–25.3 g/m3, respectively), and categories. The analysis represents 10,966 individual data which indicate a continuous progression of the performance for all categories. Principal component analysis reveals that the slowest XCO performance was resulting from high ambient temperature and absolute humidity. Regressions revealed that only altitude (P < 0.0001) have a direct linear negative effect on XCO average speed. A significant negative interaction effect of altitude with absolute humidity (P < 0.0001) on XCO average speed was also found. In addition, the higher the absolute humidity, the higher is the impact of ambient temperature (P < 0.0001) on XCO average speed. While XCO performance progressed over time regardless of the categories, results also indicate that altitude, ambient temperature, and absolute humidity negatively impact XCO performance. Abbreviations LOESS: local estimated scatterplot smoothing; PCA: Principal component analysis; UCI: Union Cycliste Internationale; U23: under-23; VO2max: maximal oxygen uptake; XCO: cross-country cycling.

This systematic review and meta-analysis aimed to assess studies which have investigated cooling methodologies, their timing and effects, on endurance exercise performance in trained athletes (Category 3; VO2max ≥ 55 mL·kg·min−1) in hot environmental conditions (≥28 °C). Meta-analyses were performed to quantify the effects of timings and methods of application, with a narrative review of the evidence also provided. A computer-assisted database search was performed for articles investigating the effects of cooling on endurance performance and accompanying physiological and perceptual responses. A total of 4129 results were screened by title, abstract, and full text, resulting in 10 articles being included for subsequent analyses. A total of 101 participants and 310 observations from 10 studies measuring the effects of differing cooling strategies on endurance exercise performance and accompanying physiological and perceptual responses were included. With respect to time trial performance, cooling was shown to result in small beneficial effects when applied before and throughout the exercise bout (Effect Size: −0.44; −0.69 to −0.18), especially when ingested (−0.39; −0.60 to −0.18). Current evidence suggests that whilst other strategies ameliorate physiological or perceptual responses throughout endurance exercise in hot conditions, ingesting cooling aids before and during exercise provides a small benefit, which is of practical significance to athletes’ time trial performance.

Endurance exercise performance is impaired in a hot and humid environment. This study compared the effects of cold water immersion, with (CMWI) and without (CWI) menthol, on the recovery of cycling performance. Eight heat-acclimatized cyclists (age, 24.1 ± 4.4 years; mass, 65.3 ± 5.2 kg) performed 2 randomized sessions, each consisting of a 20-min cycling trial (T1) followed by 10 min of immersion during recovery and then a second 20-min cycling trial (T2). Mean power output and perceived exertion (RPE) were recorded for both trials. Rectal (Trec) and skin temperatures were measured before and immediately after T1, immersion, and T2. Perceived thermal sensation (TS) and comfort were measured immediately after T1 and T2. Power output was significantly improved in T2 compared with T1 in the CMWI condition (+15.6%). Performance did not change in the CWI condition. After immersion, Trec was lower in CWI (-1.17 °C) than in CMWI (-0.6 °C). TS decreased significantly after immersion in both conditions. This decline was significantly more pronounced in CMWI (5.9 ± 1 to 3.6 ± 0.5) than in CWI (5.6 ± 0.9 to 4.4 ± 1.2). In CMWI, RPE was significantly higher in T1 (6.57 ± 0.9) than in T2 (5.14 ± 1.25). However, there was no difference in TC. This study suggests that menthol immersion probably (i) improves the performance of a repeated 20-min cycling bout, (ii) decreases TS, and (iii) impairs thermoregulation processes.

This study investigated whether the combination of internal precooling (PC) and internal midcooling (MC cooling during exercise) would enhance performance more than MC alone. 9 trained males completed two 30-km cycling trials in a hot and humid environment (WBGT: 29 +/- 0.7 degrees C, 80 +/- 0.02% relative humidity). For 30min before exercise, the subjects sat quietly and drank water at 23 degrees C (MC) or 3 degrees C (PC+MC). During the MC and PC+MC time trials, they drank an ice-slush/menthol beverage (i.e., 0.025% menthol). Trial time, gastrointestinal temperature (T-co), heart rate (HR), rating of perceived exertion (RPE), thermal sensation (TS), and thermal comfort (TC) were assessed. Trial time was not significantly different (P>0.05) between MC and PC+MC (3737 +/- 552s and 3815 +/- 455s). Before exercise, T(co)was lower with precooling (37.0 +/- 0.3 degrees C; P<0.02). During exercise, no between-trial differences were noted for T-co, HR, RPE, TC or TS, but RPE was significantly lower with PC+MC in the latter stages (P<0.05). (1) Cold beverage intake before exercise did not improve the subsequent exercise performance with ice-slush/menthol beverage intake, and (2) despite no improvement in performance, RPE declined in the latter stages of exercise in the condition of PC+MC, suggesting that this combination might be beneficial for longer exercise.

The authors explored the effects of open water swimming in a tropical environment on both core temperature (T c) and thermal perceptions of high-level swimmers during an official international 10-km race and two 5-km swimming tests. The swimmers drank neutral water (i. e., 28.0±3.0°C) ad libitum every 2,000 m during Competition, whereas the ingested volume was imposed in the 5-km tests: every 1,000 m, they drank 190 mL of cold water (CW, 1.1±0.7°C) or neutral water (NW, 28.0±3.0°C). They also self-rated their thermal comfort and sensation (TC and TS), and their T c was recorded. The study demonstrated that adequate fluid intake significantly decreased T c in swimmers swimming at race pace in hot water (i. e., 37.5±0.3°C vs. 38.3±0.4°C, in NW vs. Competition, respectively). This effect was more pronounced with cold water (i. e., 36.7±1.1°C, in CW). No significant changes were noted in mean heart rate (i. e., 145±5, 143±4 and 141±5 bpm for NW, CW and Competition, respectively). Further studies are needed to explore the effect of this cooling method on the performances of international swimmers during tropical swimming events.

PURPOSE:A recent laboratory study demonstrated that the ingestion of a cold/menthol beverage improved exercise performance in a hot and humid environment during 20 km of all-out cycling. Therefore, the aim of this study was to determine whether the ingestion of cold water/ice-slurry with menthol would improve performance in hot and humid outdoor conditions.METHODS:Ten trained males completed three trials of five blocks consisting of 4-km cycling and 1.5-km running. During warm-up, every block and recovery, the athletes drank 190 ml of aromatized (i.e., with 0.05 mL of menthol) beverage at three temperatures: Neutral (ambient temperature) (28.7°C±0. 5°C), Cold (3.1°C±0.6°C) or Ice-slurry (0.17°C±0.07°C). Trial time, core temperature (Tco), heart rate (HR), rate of perceived exertion (RPE), thermal sensation (TS) and thermal comfort (TC) were assessed.RESULTS:Ice-slurry/menthol increased performance by 6.2% and 3.3% compared with neutral water/menthol and cold water/menthol, respectively. No between-trial differences were noted for Tco, HR, RPE, TC and TS was lower with ice-slurry/menthol and cold water/menthol compared with neutral water/menthol.CONCLUSION:A low drink temperature combined with menthol lessens the performance decline in hot/humid outdoor conditions (i.e., compared with cold water alone). Performances were better with no difference in psycho-physiological stress (Tco, HR and RPE) between trials. The changes in perceptual parameters caused by absorbing a cold/menthol beverage reflect the psychological impact. The mechanism leading to these results seems to involve brain integration of signals from physiological and psychological sources.

Introduction. - Colors may influence motor performance.Objective. - The present study aimed to compare the effects of perception of red and green environments on physical (performance and heart rate) and psychological (perceived effort, anxiety and enjoyment) parameters during cycling trials.Method. - Ten cyclists achieved two identical series of three randomized 7-minute trials on home trainers, during which they were continuously exposed to red, green, and gray environments. Covered distance and heart rate were recorded during each trial, after which participants answered items intended to assess perceived effort, anxiety, and enjoyment experienced during the trial.Results. - Results showed that covered distance (ps <= .02) and heart rate (ps <= .03) were lower in the red environment than in the gray and green environments. Enjoyment was higher in the green environment than in the red environment (p = .006). The colored environments did not influence perceived effort and anxiety (ps > .05).Conclusion. - This study is the first to show that perceiving red is detrimental for motor performance over an extended period of time. (C) 2015 Elsevier Masson SAS. All rights reserved.

BACKGROUND Human thermal responses during prolonged whole-body immersion in cold water are of interest for the military, especially French SEALS. This study aims at describing the thermo-physiological responses. METHODS There were 10 male military divers who were randomly assigned to a full immersion in neutral (34 degrees C), moderately cold (18 degrees C), and cold (10 degrees C) water wearing their operational protective devices (5.5 mm wetsuit with 3.0 mm thick underwear) for 6 h in a static position. Rectal temperature (T(re)) and 14 skin temperatures (T(sk)), blood analysis (stress biomarkers, metabolic substrates), and oxygen consumption (Vo2) were collected. RESULTS At 34 degrees C, there were no significant modifications of the thermo-physiological responses over time. The most interesting result was that rates of rectal temperature decrease (0.15 +/- 0.02 degrees C x min(-1)) were the same between the two cold stress experimental conditions (at 18 degrees C and 10 degrees C). At the final experiment, rectal temperature was not significantly different between the two cold stress experimental conditions. Mean T(sk) decreased significantly during the first 3 h of immersion and then stabilized at a lower level at 10 degrees C (25.6 +/- 0.8 degrees C) than at 18 degrees C (29.3 +/- 0.9 degrees C). Other results demonstrate that the well-trained subjects developed effective physiological reactions. However, these reactions are consistently too low to counterbalance the heat losses induced by cold temperature conditions and long-duration immersion. CONCLUSION This study shows that providing divers with thermal protection is efficient for a long-duration immersion from a medical point of view, but not from an operational one when skin extremities were taken into account.

PURPOSE:This study compares the effects of neutral temperature, cold and ice-slush beverages, with and without 0.5% menthol on cycling performance, core temperature (Tco) and stress responses in a tropical climate (hot and humid conditions).METHODS:Twelve trained male cyclists/triathletes completed six 20-km exercise trials against the clock in 30.7°C±0.8°C and 78%±0.03% relative humidity. Before and after warm-up, and before exercise and every 5 km during exercise, athletes drank 190 mL of either aromatized (i.e., with 0.5 mL of menthol (5 gr/L)) or a non-aromatized beverage (neutral temperature: 23°C±0.1°C, cold: 3°C±0.1°C, or ice-slush: -1°C±0.7°C). During the trials, heart rate (HR) was continuously monitored, whereas core temperature (Tco), thermal comfort (TC), thermal sensation (TS) and rate of perceived exertion (RPE) were measured before and after warm-up, every 5 km of exercise, and at the end of exercise and after recovery.RESULTS:Both the beverage aroma (P<0.02) and beverage temperature (P<0.02) had significant and positive effects on performance, which was considerably better with ice-slush than with a neutral temperature beverage, whatever the aroma (P<0.002), and with menthol vs non-menthol (P<0.02). The best performances were obtained with ice-slush/menthol and cold/menthol, as opposed to neutral/menthol. No differences were noted in HR and Tco between trials.CONCLUSION:Cold water or ice-slush with menthol aroma seems to be the most effective beverage for endurance exercise in a tropical climate. Further studies are needed to explore its effects in field competition.

We hypothesized that the changes in muscle temperature and interstitial pressure during thermoneutral immersion may affect the reflex adaptation of the motor drive during static contraction, assessed by the decrease in median frequency (MF) of electromyogram (EMG) power spectrum. Ten subjects were totally immerged for 6 h at 35°C and repeated maximal voluntary contraction (MVC) and submaximal (60% MVC) leg extensions sustained until exhaustion. In vastus lateralis (VL) and soleus (SOL) muscles, the compound muscle potential evoked by muscle stimulation with single shocks (M-wave) was recorded at rest, and MF of surface EMG was calculated during 60% MVCs. We measured lactic acid and potassium venous blood concentrations and calculated plasma volume changes. Data were compared to those obtained in the same individuals exercising at 35°C under dry conditions where the MF decrease during 60% MVCs was modest (−4 to−5%). During immersion, the rectal temperature remained stable, but the thigh and calf surface temperatures significantly increased. Lactic acid and potassium concentrations did not vary, but plasma volume decreased from the 180th min of immersion. The M-wave did not vary in VL but was prolonged in SOL from the 30th min of immersion. From the 220th min of immersion, the maximal MF decrease was majored in both muscles (−18 to −22%). Thus, compared to the dry condition, total body thermoneutral immersion enhances fatigue-induced EMG changes in leg muscles, perhaps through the activation of warm-sensitive muscle endings and/or the changes in interstitial pressure because of vasodilatation.

Background: Oxygen divers undergo environmental stressors such as immersion, ventilation with scuba, cold exposure, and increased ambient pressure. All of these stressors may be responsible for acute hemodynamic modifications. We hypothesized that repeated hyperbaric hyperoxia exposure induces long-term cardiovascular modifications. Methods: A Doppler echocardiography was conducted on 20 military oxygen divers (average 12 yr diving experience) and compared with 22 controls. Parameters known to be modified by acute hyperoxic exposure, such as left ventricular (LV) function (systolic and diastolic) and arterial compliance, were analyzed. Results: Controls and divers were matched appropriately for age and height, although the divers had a higher body mass index and aerobic capacity. Left atrial and left ventricular diameters did not differ between the two groups. On the other hand, left ventricular mass was significantly higher in the elite military divers (209 +/- 43 g) in comparison with the control group (172 +/- 48 g), even when LV mass was indexed to body surface area. Left ventricular systolic and diastolic function indices, stroke volume, cardiac index, peripheral vascular resistance, and systemic compliance were comparable between the two groups. Conclusion: A greater LV mass was observed in oxygen military divers. The echocardiographic differences between divers and controls could be attributed to the high level physical training undertaken by the military divers. Some stressors, such as cold water immersion, repeated hyperoxic exposures, scuba breathing, and long distance swimming, could have participated to the echocardiographic findings in oxygen divers.