A Gram-stain-negative, aerobic bacterium, designated HXT-9T, was isolated from a river sediment. Cells were rod-shaped and non-motile. Growth occurred at 10–42 ℃ (optimum 30 ℃), at pH 5.0–8.0 (optimum pH 7.0) and with NaCl concentrations of 0–2.5% (optimum 0.5%). Phylogenetic trees based on 16S rRNA gene sequences showed that strain HXT-9T belonged to the genus Parvibaculum, and showed highest similarities to P. lavamentivorans KCTC 22775T (96.1%), followed by P. hydrocarboniclasticum EPR 92T (94.8%) and P. indicum P31T (93.6%). The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain HXT-9T and P. lavamentivorans KCTC 22775T were 75.2% and 20.8%, respectively. The G + C content of strain HXT-9T genome was 62.1 mol%. The major fatty acids (> 10%) were summed feature 8 (C18:1ω7c and/or C18:1ω6c), C19:0 cyclo ω8c and C16:0 3-OH. The major respiratory quinone was ubiquinone 11 (Q-11). The major polar lipids were DPG (diphosphatidylglycerol), PG (phosphatidylglycerol), PME (phosphatidylmonomethylethanolamine), PE (phosphatidylethanolamine), PC (phosphatidylcholine) and AL (unidentified aminolipids). The phylogenetic analysis and physiological and biochemical data showed that strain HXT-9T represents a novel species in the genus Parvibaculum, for which the name P. sedimenti sp. nov. is proposed. The type strain is HXT-9T (= CCTCC AB 2019273T = KCTC 72547T).

为获得高性能磷吸附材料,实现污染水体磷元素的高效回收利用,以食用菌菌渣为原料,研究改性条件对生物炭磷吸附性能的影响,分别为:KOH改性生物炭(BCK)、镁改性生物炭(BCM)、钙改性生物炭(BCC)、钙镁改性生物炭(BC/CM)及鸡蛋壳改性生物炭(EC).实验对比发现:(1)热解温度是影响磷吸附效果的重要因素,不同温度制备的生物炭吸附性能差异较大.在600—900℃温度下制备的生物炭中,800℃条件下制备的生物炭吸附性能最佳,这是因为较高的温度有利于生物炭孔隙率的提高,但温度过高时亦会导致生物炭孔隙结构的坍塌;(2)鸡蛋壳与菌渣直接混合制备的生物炭一步实现扩孔与金属氧化物负载,磷吸附能力高于另外3组处理组,去除效果最佳,钙镁双金属浸渍改性生物炭对磷的吸附效果显著优于镁改性及KOH改性,4种改性生物炭的磷吸附能力由高到低依次为EC>BC/CM>BCC>BCM>BCK;(3)在蛋壳与生物炭的比例小于2?1时,改性生物炭的磷吸附能力随蛋壳添加量的增加而增大.然而,进一步增加蛋壳的添加量会导致碳含量的降低,且CaO在生物炭表面的沉积会破坏生物炭的多孔结构,降低其对磷的吸附能力.此外,实验还发现以蛋壳与生物炭比例为1?1制备的生物炭对磷的吸附能力最高,可达159.69 mg·g?1,是未改性生物炭的5倍.这些结果表明,蛋壳改性生物炭在废水中磷的回收利用方面具有很大的潜力.

旨在对农村有机生活垃圾厌氧发酵产沼气的潜力进行研究.对江苏省徐州市沛县大屯街道王庄村生活垃圾分类处置中心每月1次采集样品,并对一周年十二个月份的生活垃圾样品进行批式中温厌氧发酵产气潜力研究,考察农村生活垃圾作为发酵原料周年沼气产量和甲烷产量的变化,各月份试验组的累积沼气产气量范围为447～1398 mL、累积甲烷产气量范围为215～865 mL.并用SPSS数据分析发现农村生活垃圾含水率、总磷TP、总钾TK、总氮TN、有机质、C/N、半纤维素、纤维素、木质素的含量均对产沼气潜力有影响,其中纤维素含量会对产沼气潜力有显著的正向影响关系,而TP会对产气潜力产生显著的负向影响关系,最后,该研究根据生活垃圾理化性质数据基础上建立了多元线性逐步回归最优方程,可根据农村有机生活垃圾的含水率、纤维素和木质素的含量预测其厌氧发酵产沼气潜力.