BackgroundControl and elimination of human African trypanosomiasis (HAT) can be accelerated through the use of diagnostic tests that are more accurate and easier to deploy. The goal of this work was to evaluate the immuno-reactivity of antigens and identify candidates to be considered for development of a simple serological test for the detection of Trypanosoma brucei gambiense or T. b. rhodesiense infections, ideally both.Methodology/Principal FindingsThe reactivity of 35 antigens was independently evaluated by slot blot and ELISA against sera from both T. b. gambiense and T. b. rhodesiense infected patients and controls. The antigens that were most reactive by both tests to T. b. gambiense sera were the membrane proteins VSG LiTat 1.3, VSG LiTat 1.5 and ISG64. Reactivity to T. b. rhodesiense sera was highest with VSG LiTat 1.3, VSG LiTat 1.5 and SRA, although much lower than with T. b. gambiense samples. The reactivity of all possible combinations of antigens was also calculated. When the slot blot results of 2 antigens were paired, a VSG LiTat 1.3-ISG75 combination performed best on T. b. gambiense sera, while a VSG LiTat 1.3-VSG LiTat 1.5 combination was the most reactive using ELISA. A combination of SRA and either VSG LiTat 1.3 or VSG LiTat 1.5 had the highest reactivity on T. b. rhodesiense sera according to slot blot, while in ELISA, pairing SRA with either GM6 or VSG LiTat 1.3 yielded the best results.ConclusionsThis study identified antigens that were highly reactive to T. b. gambiense sera, which could be considered for developing a serological test for gambiense HAT, either individually or in combination. Antigens with potential for inclusion in a test for T. b. rhodesiense HAT were also identified, but because their reactivity was comparatively lower, a search for additional antigens would be required before developing a test for this form of the disease.
Citation for published version (APA): Biéler, S., Waltenberger, H., Barrett, M. P., McCulloch, R., Mottram, J. C., Carrington, M., Schwaeble, W., McKerrow, J., Phillips, M. A., Michels, P. A., Büscher, P., Sanchez, J-C., Bishop, R., Robinson, D. R., Bangs, J., Ferguson, M., Nerima, B., Albertini, A., Michel, G., ... Ndung'u, J. M. (2016). Evaluation of antigens for development of a serological test for Human African Trypanosomiasis. PLoS ONE, 11(12), 1-23. [e0168074]. https://doi.org/10.1371/journal.pone.0168074
Derrick Roy Robinson合作论文数Univ Bordeaux 2, CNRS, UMR 5234, F-33076 Bordeaux, France2Richard Bishop合作论文数2
Michael P Barrett合作论文数Wellcome Centre for Integrative Parasitology, Institute of Infection, Immunity & Inflammation, University of Glasgow2Biéler Sylvain合作论文数2
Jeremy Mottram合作论文数York Biomedical Research Institute, Department of Biology, University of York2
James McKerrow合作论文数Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego;Division of Infectious Disease and Revelle College, University of California San Diego;Center for Discovery and Innovation in Parasitic Diseases2
Wilhelm Schwaeble合作论文数Department of Veterinary Medicine, Cambridge Veterinary School, University of Cambridge2Audrey Albertini合作论文数2
Jean-Charles Sanchez合作论文数Department of Human Protein Science, University of Geneva;ABCDx SA2
Barbara Nerima合作论文数2
Michael Ferguson合作论文数School of Life Sciences, University of Dundee2Philippe Buscher合作论文数Institute of Tropical Medicine2Magdalena Radwanska合作论文数Lab Biomed Res, Univ Ghent2James D. Bangs合作论文数Department of Physiological Chemistry, Johns Hopkins University School of Medicine2Richard Mcculloch合作论文数Centre for Molecular Parasitology;University of Glasgow;Glasgow Biomedical Research Centre;Glasgow Biomedical Research Centre, University of Glasgow2M Carrington合作论文数1
Paul Michels合作论文数Catholic Univ Louvain, Trop Dis Res Unit, de Duve Inst, B-1200 Brussels, Belgium1
Mark Carrington合作论文数Department of Biochemistry, University of Cambridge1
Margaret Phillips合作论文数Department of Biochemistry, The University of Texas Southwestern Medical Center;Medical School, The University of Texas Southwestern Medical Center1
Joseph Mathu Ndung'u合作论文数1
