The electrophoretic mobility (EPM) of cells from rat ovary carcinoma lines differring in ploidity and metastatic capacity has been studied. It was shown that the reduction of EPM of metastatic cells after treatment with hyaluronidase and alcian blue (AB) sorption was 2-3 times as much as the nonmetastatic cells. The data obtained by scanning electron microscopy have shown marked differences in cell surface microstructure of the metastatic and nonmetastatic cells after treatment with hyaluronidase and AB.

A study was made of the antioxidative activity (AOA), of the activity of glytathionreductase and catalase, and of SH-group concentration in suspensions of intact cells from eight fractions of the rat ascitic ovary tumor. A correlative bond was detected between AOA, the activity of antioxidative enzymes, and SH-group concentration. It was shown that antioxidative properties of the fractions containing resting (G0) and polyploid cells were higher than those of proliferating diploid cells.

Changes in concentrations of protein- and lipid-associated sialic acids (PASA, LASA) in the blood serum, ascitic fluid and cells of clonal lines in ovary tumor of rats have been studied. A considerable increase of PASA and LASA concentrations in serum as against the standard has been found. The concentrations correlate with those in the ascitic fluid, but not in the tumor cells. Maximum frequency of the circulating PASA and LASA concentrations was determined in rats with polyploid unmetastatic tumors, and minimum - in animals with metastatic ovary tumors irrespective of the tumor ploidity level. Possible relation between concentration of circulating sialoconjugates and tumor cell composition is under discussion.

The peripheral blood leukocytes in chronic myeloid leukaemia (CML) patients and healthy donors are separated on ficoll-verografin one-step gradients with 1.077 g/ml density. It is shown that 95-98% of donor granulocytes had the density above 1.077 g/ml. Granulocytes of CML patients consisted of two populations having the density above and below 1.077 g/ml (high density granulocytes-HDG, low density granulocytes-LDG). The electrophoretic mobility (EPM) of LDG is 8-34% higher than that of HDG. As a result of EPM definition of granulocytes affected by hyaluronidase, neuraminidase and RNase it is shown that the high EPM of LDG is due to an increase in the density of sialic acids on their surface.

The relation was studied between the density of the electrical charge of the surface (DECS) and the karyological structure of the rat ovary tumour (OT) cells. For this purpose the sedimentation rate and electrical mobility of cells from two OT clones were measured. The OT1 and OT2 clones differed in their modal chromosome numbers (44 and 80-90, respectively). An increase in DECS by 4-5% on the average occurs in cells with the chromosome set close to the tetraploid one. The further increase in the chromosome number to the octaploid one does not change DECS. The results obtained show stability of DECS with significant changes in the structure of the OT cells karyotype.

The electrophoretic mobility of the ascitic tumor cells with different rates of sedimentation was measured by photographic registration of summarizing traces in the electrophoretic chamber. Changes in electrophoretic mobility of these cells during tumour growth are described. A conclusion is made that these changes are not connected with the proliferative activity of the tumour. It was shown that cells with low densities appeared in the late period of the tumor growth.

The described method of analysis of cell populations' heterogeneity involves two parameters simultaneously: the sedimentation velocity and the electrophoretic mobility of cells. The method is based on the photographic registrarion of resulting traces of cell velocity in the fields of gravition and electricity with consequence of the elements of these traces on the photographs. The experimental data on the distribution of these parameters in the ascitic tumor cell population are given.