TransfusionVolume 45, Issue 9 p. 1544-1546 Frozen-and-thawed allogeneic platelet gels for treating postoperative chronic wounds Paolo Perseghin MD, Paolo Perseghin MD e-mail: [email protected]Search for more papers by this authorGianalfredo Sciorelli MD, Gianalfredo Sciorelli MD Servizio di Immunoematologia e Trasfusionale Ospedale San GerardoSearch for more papers by this authorDaniela Belotti PhD, Daniela Belotti PhD Divisione di Ematologia Laboratorio di Emostasi e Trombosi Università di Milano-Bicocca Ospedale San GerardoSearch for more papers by this authorTiziana Speranza BSc, Tiziana Speranza BSc Divisione di Ematologia Laboratorio di Emostasi e Trombosi Università di Milano-Bicocca Ospedale San GerardoSearch for more papers by this authorEnrico M. Pogliani MD, Enrico M. Pogliani MD Divisione di Ematologia Laboratorio di Emostasi e Trombosi Università di Milano-Bicocca Ospedale San GerardoSearch for more papers by this authorOrazio Ferro MD, Orazio Ferro MD Divisione di Cardiochirurgia Università di Milano-Bicocca Ospedale San Gerardo Monza, ItalySearch for more papers by this authorMonica Gianoli MD, Monica Gianoli MD Divisione di Cardiochirurgia Università di Milano-Bicocca Ospedale San Gerardo Monza, ItalySearch for more papers by this authorFabiano Porta MD, Fabiano Porta MD Divisione di Cardiochirurgia Università di Milano-Bicocca Ospedale San Gerardo Monza, ItalySearch for more papers by this authorGiovanni Paolini PhD, MD, Giovanni Paolini PhD, MD Divisione di Cardiochirurgia Università di Milano-Bicocca Ospedale San Gerardo Monza, ItalySearch for more papers by this author Paolo Perseghin MD, Paolo Perseghin MD e-mail: [email protected]Search for more papers by this authorGianalfredo Sciorelli MD, Gianalfredo Sciorelli MD Servizio di Immunoematologia e Trasfusionale Ospedale San GerardoSearch for more papers by this authorDaniela Belotti PhD, Daniela Belotti PhD Divisione di Ematologia Laboratorio di Emostasi e Trombosi Università di Milano-Bicocca Ospedale San GerardoSearch for more papers by this authorTiziana Speranza BSc, Tiziana Speranza BSc Divisione di Ematologia Laboratorio di Emostasi e Trombosi Università di Milano-Bicocca Ospedale San GerardoSearch for more papers by this authorEnrico M. Pogliani MD, Enrico M. Pogliani MD Divisione di Ematologia Laboratorio di Emostasi e Trombosi Università di Milano-Bicocca Ospedale San GerardoSearch for more papers by this authorOrazio Ferro MD, Orazio Ferro MD Divisione di Cardiochirurgia Università di Milano-Bicocca Ospedale San Gerardo Monza, ItalySearch for more papers by this authorMonica Gianoli MD, Monica Gianoli MD Divisione di Cardiochirurgia Università di Milano-Bicocca Ospedale San Gerardo Monza, ItalySearch for more papers by this authorFabiano Porta MD, Fabiano Porta MD Divisione di Cardiochirurgia Università di Milano-Bicocca Ospedale San Gerardo Monza, ItalySearch for more papers by this authorGiovanni Paolini PhD, MD, Giovanni Paolini PhD, MD Divisione di Cardiochirurgia Università di Milano-Bicocca Ospedale San Gerardo Monza, ItalySearch for more papers by this author First published: 26 August 2005 https://doi.org/10.1111/j.1537-2995.2005.00570.xCitations: 10Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat REFERENCES 1 Zimmermann R, Jakubietz R, Jakubietz M, et al. Different preparation methods to obtain platelet component as a source of growth factors for local application. Transfusion 2001; 41: 1217-24. 2 Mazzucco L, Medici M, Serra M, et al. The use of autologous platelet gel to treat difficult-to-heal wounds: a pilot study. Transfusion 2004; 44: 1013-8. 3 Crovetti G, Martinelli G, Issi M, et al. Platelet gel for healing cutaneous chronic wounds. Transfus Sci 2004; 300: 141-51. 4 Kevy SV, Jacobson MS. Comparison of methods for point of care preparation of autologous platelet gel. J Extra Corpor Technol 2004; 36: 28-35. 5 Robson MC. The role of growth factors in the healing of chronic wounds. Wound Repair Regen 1997; 5: 12-7. Citing Literature Volume45, Issue9September 2005Pages 1544-1546 ReferencesRelatedInformation

BACKGROUND: The wide diffusion of multicomponent collection in donor apheresis has led to the yielding of different components, such as plasma‐reduced platelet‐pheresis at high PLT concentration. We investigated whether this collection modality could induce more PLT activation compared to standard plateletpheresis.STUDY DESIGN AND METHODS: Forty‐one plateletpheresis collections (20 Trima and 21 Spectra LRS Turbo v.7.0, COBE) were evaluated. Donor, procedure, and product data were recorded. ADP, collagen, and U46619 (a thromboxane‐A2 analog)‐induced PLT aggregation was investigated in basal (donor) and final (plateletpheresis unit) samples. The expression of PLT activation marker P‐selectin (CD62P) was studied using flow cytometry in basal and final samples. In all cases, P‐selectin was investigated in final samples after stimulation with ADP to assess for a possible further release of the antigen. Four additional plateletpheresis procedures were performed in donors from Group A, using the traditional, nonplasma‐reduced program.RESULTS: Plateletpheresis obtained by means of the Trima device showed a lower response to in‐vitro induced PLT aggregation and a higher percentage of P‐selectin‐expressing PLT when compared to products obtained using the Spectra device. Moreover, P‐selectin release after ADP stimulation was reduced in plateletpheresis units obtained using the Trima device. These differences disappeared when a nonplasma‐reduced collection program was used. In‐vivo evaluation did not detect any difference between platelepheresis obtained by means of the two cell separators.CONCLUSIONS: Plateletpheresis units obtained by means of multicomponent collection show a higher degree of PLT activation compared to traditional plateletpheresis procedures when high‐concentration plasma‐reduced products are collected. Randomized clinical studies are needed to assess the real impact of these findings in terms of in‐vivo efficacy of plasma‐reduced plateletpheresis units.

Lymphocyte subsets were determined in 20 packed red blood cell units (PRC) before and after filtration (FPRC) with the Pall Leukotrap RC inline filter system; 10 units were prepared by low spin and platelet rich plasma (PRP) removal (Group A) and 10 with high spin, plasma and buffy-coat (BC) removal (Group B). Flow cytometry was employed for white blood cell (WBC) enumeration and phenotype analysis. Median WBCs in prefiltered units was 2.08 x 10(9) (Group A) vs. 0.8 x 10(9) (Group B) (p < 0.0001). Five Group A and three Group B filtered units had WBC counts above the limit of detection (LD), median values being 25.59 and 3.08 x 10(3), respectively. Whereas CD3+, CD3+CD4+ and CD3+CD8+ lymphocyte subsets were assessable in 20-40% of Group A units, inline filtration of Group B units lowered lymphocytes below the LD of the present study. Post-filtration CD19+ lymphocytes were below the LD in all the 20 units.

BACKGROUND AND OBJECTIVES:Extracorporeal circuits made of artificial substances may induce blood cells and humoral activation. Negatively charged surfaces may activate Factor XII and the prekallikrein-kinin cascade, resulting in bradykinin (BK) production. BK has been considered to be involved in severe hypotensive reactions occurring during therapeutic apheresis in patients taking angiotensin-converting enzyme (ACE) inhibitors or in those receiving platelet transfusion. In this study we investigated BK production during donor plasmapheresis procedures. PATIENTS AND METHODS:Eighteen volunteer donors entered the study protocol. Nine of them were taking ACE inhibitors. Their blood pressure (BP) was monitored both pre- and post-apheresis, and BK determination was carried out using a competitive enzyme immunoassay (EIA), in plasma samples collected both during and at completion of the procedure. In addition, a limited number of thawed plasma units were checked for BK. RESULTS:No side-effects were observed during the procedures. However, donors taking ACE inhibitors showed a higher variation of their systolic BP compared to those who were not taking ACE inhibitors, while diastolic BP percentage variations did not differ significantly between the two groups. The BK concentration was considerably higher in donors taking ACE inhibitors: 183 +/- 26 versus 82 +/- 6 ng/ml (P < 0.0001) after the first collection cycle and 142 +/- 20 versus 65 +/- 11 ng/ml (P < 0.0001) in the final samples. BK was also detected, at a lower concentration (15 ng/ml), in one out of four thawed plasma units obtained from donors taking ACE inhibitors and at 1 ng/ml in one out of two thawed plasma units from the control group. CONCLUSION:Donors taking ACE inhibitors and undergoing plasmapheresis showed higher levels of BK compared to the control group. Furthermore, the detection of BK in plasma units after a freeze-thaw procedure might explain the sudden hypotensive reaction occurring during therapeutic plasma exchange when plasmapheresis units are adopted as substitution fluids. Further investigations are needed to assess the real clinical importance of the presence of BK in plasma units.

BACKGROUND: The importance of transfusing WBC-reduced blood components is widely recognized, as it reduces the risk of alloimmunization and transfusion-transmitted CMV infections, The latest generation of cell separators allows the collection of WBC-reduced apheresis platelet concentrates (APCs).MATERIALS AND METHODS: Consecutive APCs (n = 232) were retrospectively evaluated: 163 collected with the Spectra LRS [leukocyte-reduction system] Version 5.1 (Group A) and 69 with the LRS Turbo Version 7.0 (Group B) (both: CODE BCT). Donor peripheral blood count, procedure data, platelet yield, collection efficiency (CE), and residual WBC count in APCs were recorded.RESULTS: The platelet yield was higher in Group B than in Group A: 5.5 +/- 1.4 versus 4.4 +/- 1.1, p<0.0001; residual WBCs were <5 x 10(6) in 99.4 percent of Group A APCs and in 97.1 percent of Group B APCs. CE was higher in Group B than in Group A: 51.4 +/- 8.7 versus 43.6 +/- 6.3, p<0.0001. Moreover, a correlation between predonation platelet count and platelet yield was observed in both groups. A double product (platelet yield >6.0 x 10(11)) was obtained in 28.9 percent of Group B APCs and in 9.2 percent of Group A APCs.CONCLUSIONS: The Spectra LRS Turbo version 7.0 release showed a better CE and resulted in a higher platelet harvest than did the LRS version 5.1. High predonation platelet counts allow a higher platelet yield.

In order to improve the process control of bedside filtration, a multisampling procedure was evaluated. Serial samples of postfiltration blood were collected, and white blood cells (WBCs) were counted by a microdroplet fluorochromatic assay. The kinetics of the bedside filter efficiency was shown to be nonlinear, with a saturation-like pattern owing to leukocyte escaping mostly during the second half of the filtration procedure. Multisample procedure allows the computation of the actual amount of filter-escaping leukocytes, while evaluation of filter-escaping leukocytes computed on the basis of a single-point sample (postfiltration terminal segment) may give misleading results and an overestimation of the number of filter-escaping WBCs.

In recent years peripheral blood stem cell (PBSC) collection for allogeneic or autologous transplantation has experienced an increased use in the onco-hematological setting. The latest generation cell separators allow a satisfactory and safe PBSC collection. Nevertheless, as in all therapeutic apheresis procedures, patients may experience procedure-related side-effects, mainly vasovagal reactions or symptoms related to hypocalcemia and/or hypomagnesemia. We investigated electrolyte changes in 18 patients, with a median age of 46 years (range 7-62), undergoing PBSC collection from January to April 1998. A significant decrease in total calcium in the final sample (9.65 +/- 0.7 mg/dL) with respect to the basal one (9.2 +/- 0.6 mg/dL, P < 0.05) was observed; also ionized calcium decreased markedly from the first sample drawn at +30 minutes: 1.22 +/- 0.14 vs. 1.03 +/- 0.15 mmol/L (P < 0.05), and a highly significant difference emerged when basal value were compared to the final value: 1.22 +/- 0.14 vs. 0.94 +/- 0.13 mmol/L (P < 0.0001). Similar findings affected potassium concentration: 4.1 +/- 0.4 vs. 3.3 +/- 0.3 mEq/L (P < 0.0001). Three out of eighteen patients (16.7%) reached a final potassium level <3.0 mEq/L, and eight out of eighteen (44.5%) showed a potassium concentration decrease >20% with respect to the basal value. A mild metabolic alkalosis occurred during the procedure: pH increased from 7.35 +/- 0.02 to 7.43 +/- 0.028 (P < 0.001), and plasma bicarbonate concentration increased from 27.48 +/- 2.21 to 32.44 +/- 2.52 mmol/L (P < 0.01). Sodium and chloride did not differ in the final sample with respect to the basal sample. None of our patients experienced clinically relevant side effects related to severe electrolyte changes (i.e., >20% with respect to the basal value). Because our current therapeutic schedules include patients older than 50 years in the PBSC collection and transplantation program and since it is well known that subclinical myocardial disease may occur in up to 4% of middle-aged males, we suggest that patients aged 50 or older undergoing PBSC collection procedures be carefully monitored in order to identify significant electrolyte variation, especially if they present with low serum potassium levels. However, further investigation of larger patient series are needed to determine the clinical relevance of serum potassium changes during apheresis.

TransfusionVolume 35, Issue 10 p. 884-885 A very simple method for counting white cells in platelet units collected by apheresis Piero Borzini MD, Piero Borzini MD Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorMarlwosa Fwa BD, Marlwosa Fwa BD Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorMaria Dassi BD, Maria Dassi BD Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorPatrizia Proserpio LT, Patrizia Proserpio LT Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorAmedeo Messina LT, Amedeo Messina LT Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorLorjwzo Gangi LT, Lorjwzo Gangi LT Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorAntonio Galesi LT, Antonio Galesi LT Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorTrvana Villa LT, Trvana Villa LT Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorGianalfredo Sciorelli, Gianalfredo Sciorelli Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this author Piero Borzini MD, Piero Borzini MD Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorMarlwosa Fwa BD, Marlwosa Fwa BD Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorMaria Dassi BD, Maria Dassi BD Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorPatrizia Proserpio LT, Patrizia Proserpio LT Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorAmedeo Messina LT, Amedeo Messina LT Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorLorjwzo Gangi LT, Lorjwzo Gangi LT Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorAntonio Galesi LT, Antonio Galesi LT Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorTrvana Villa LT, Trvana Villa LT Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this authorGianalfredo Sciorelli, Gianalfredo Sciorelli Servizio di Immunoematologia e Laboratorio di Ematologia Ospea'ale San Gerardo Monza ItalySearch for more papers by this author First published: October 1995 https://doi.org/10.1046/j.1537-2995.1995.351096026374.xCitations: 5AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article.Citing Literature Volume35, Issue10October 1995Pages 884-885 RelatedInformation

Vox SanguinisVolume 64, Issue 4 p. 248-249 Platelet Cryopreservation Using DimethyIsuIfoxide/Polyethylene Glycol/Sugar Mixture as Cryopreserving Solution P. Borzini, Corresponding Author P. Borzini Servizio Immunotrasfusionale e Laboratorio di Ematologia, Ospedale San Gerardo, Monza, ItaliaServizio Immunotrasfusionale e Laboratorio di Ematologia Ospedale San Gerardo Via Solferino 16 I-20052 Monza MI (Italy)Search for more papers by this authorG. Assali, G. Assali Servizio Immunotrasfusionale e Laboratorio di Ematologia, Ospedale San Gerardo, Monza, ItaliaSearch for more papers by this authorM.R. Riva, M.R. Riva Servizio Immunotrasfusionale e Laboratorio di Ematologia, Ospedale San Gerardo, Monza, ItaliaSearch for more papers by this authorM. Bramante, M. Bramante Servizio Immunotrasfusionale e Laboratorio di Ematologia, Ospedale San Gerardo, Monza, ItaliaSearch for more papers by this authorG. Sciorelli, G. Sciorelli Servizio Immunotrasfusionale e Laboratorio di Ematologia, Ospedale San Gerardo, Monza, ItaliaSearch for more papers by this author P. Borzini, Corresponding Author P. Borzini Servizio Immunotrasfusionale e Laboratorio di Ematologia, Ospedale San Gerardo, Monza, ItaliaServizio Immunotrasfusionale e Laboratorio di Ematologia Ospedale San Gerardo Via Solferino 16 I-20052 Monza MI (Italy)Search for more papers by this authorG. Assali, G. Assali Servizio Immunotrasfusionale e Laboratorio di Ematologia, Ospedale San Gerardo, Monza, ItaliaSearch for more papers by this authorM.R. Riva, M.R. Riva Servizio Immunotrasfusionale e Laboratorio di Ematologia, Ospedale San Gerardo, Monza, ItaliaSearch for more papers by this authorM. Bramante, M. Bramante Servizio Immunotrasfusionale e Laboratorio di Ematologia, Ospedale San Gerardo, Monza, ItaliaSearch for more papers by this authorG. Sciorelli, G. Sciorelli Servizio Immunotrasfusionale e Laboratorio di Ematologia, Ospedale San Gerardo, Monza, ItaliaSearch for more papers by this author First published: May 1993 https://doi.org/10.1111/j.1423-0410.1993.tb03066.xCitations: 6AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article.Citing Literature Volume64, Issue4May 1993Pages 248-249 RelatedInformation