Tetracycline hydrochloride, a broad-spectrum antibiotic, was tested for its genotoxic potential in the Drosophila wing primordial and germ line cells. Second and third instar mwh +/+ flr(3) transheterozygous larvae were exposed to various concentrations of the test compound to record the frequency of wing spots on the eclosing adult wings. The standard Basc technique was followed for the sex-linked recessive lethal test, where Ore-R larvae of similar age were exposed to the compound. On the basis of the results obtained, it is concluded that tetracycline hydrochloride is genotoxic both in the wing primordia and germ line cells of Drosophila.

Genotoxic effects of griseofulvin, a mycotoxin with wide application in the therapy of dermatophytoses, were studied in the somatic and germ line cells of Drosophila melanogaster. Third and second instar larvae carrying genetic markers mwh and flr(3) on chromosome 3 were used to study the genotoxic effects in the wing primordial cells and the standard Base technique was followed to study the genotoxic effects in the germ line cells. On the basis of the results obtained, it is concluded that griseofulvin is genotoxic both in the somatic and germ line cells of Drosophila following chronic larval feeding.

Simazine, a triazine herbicide, was tested for its genotoxic potential in the Drosophila wing spot and sex-linked recessive lethal tests following larval exposures. 3rd and 2nd instar mwh +/+ flr(3) transheterozygous larvae were given chronic exposure to various concentrations of the test compound to record the frequency of wing spots in the eclosing adult wings. The standard Base technique was followed for the sex-linked recessive lethal test, where Oregon-R larvae of similar age were exposed to the compound. It is concluded that simazine is genotoxic both in the wing primordial and male germ line cells of Drosophila.

Two red dyes, rhodamine B and amaranth, were tested for their genotoxic effects in the somatic (wing primordia) and germ line cells of Drosophila melanogaster following the wing spot and the sex-linked recessive lethal tests. Second- and third-instar larvae, carrying suitable genetic markers, were subjected to chronic exposure to different concentrations of the test dyes. The results indicate that rhodamine is genotoxic in both somatic and germ line cells and amaranth is non-genotoxic.

The genotoxicity of two triazine herbicides, terbutryn and terbuthylazin, were studied in the Drosophila wing spot test and the sex-linked recessive lethal test following chronic larval exposures to the LD(50) and lower doses. It is concluded that both the compounds are genotoxic in the wing primordia and male germ Line cells at the LD(50).

The genotoxic potential of 2,4-dichlorophenoxyacetic acid, a commonly used chlorophenoxy herbicide, was tested in Drosophila somatic and germ-line cells following the protocols of the wing spot test and the sex-linked recessive lethal test. In the wing spot test second- and third-instar larvae, carrying genetic markers mwh and flr3, were exposed to different concentrations of the herbicide so that induced genetic changes would be phenotypically expressed as mosaic spots on the wings of eclosing adults. The Basc (Muller-5) standard technique but with larval exposure was followed for the sex-linked recessive lethal test. The results obtained indicate that the test compound is genotoxic both in the somatic and germ-line cells of Drosophila.

Atrazine (CAS No. 1912-24-9), a triazine herbicide, was tested for its genotoxic effects following larval exposures in the Drosophila somatic (wing primordia) and male germ line cells. In the somatic cell assay, mwh+/+ flr3 transheterozygous larvae were exposed to different concentrations of the test compound to observe the induction of mutant mosaic spots in the wings of developing adults. The standard Basc technique was followed for the detection of induction of recessive lethals in the male germ line. It was concluded that atrazine is genotoxic both in the somatic and germ line cells of Drosophila melanogaster.

The genotoxicity of chloral hydrate, an in vivo metabolite of 1,2,3-trichloroethylene, was studied in the wing mosaic and sex-linked recessive lethal tests in Drosophila melanogaster following larval exposures. Statistical evaluation of the data revealed that chloral hydrate is genotoxic to the wing disc cells and male germ line cells of Drosophila.