目的：探索并建立适合评价医院临床药师工作的绩效考核体系。方法参照国家卫生和计划生育委员会下发的系列文件，结合医院临床药师药学服务实际情况，选择适当的评价指标并制定出具体的考核内容。结果与结论完成了原始记录及工作记录，建立了一套规范化绩效考核体系。临床药学是医院药学发展的重要方向，客观的绩效考核体系有利于提高临床药师水平、促进临床药学学科的发展。

Object ive To explore the effect of matrine on antiproliferation and apoptosis of human cholangiocarcinoma cell line QBC939 in vitro and its mechanisms.Methods The antiproliferation rate of QBC939 cells induced by different concentrations of matrine was detected using MTT assay,the apoptosis rate of QBC939 cells was assessed by Annexin V-FITC/PI staining method,and the mitochondrial membrane potential was analyzed by Rhodamine 123 assay.The expressions of Cyto-c,Bcl-2,BAX and activated caspase-3 proteins were detected by Western Blot.Results The antiproliferation rate of QBC939 cells increased with the increasing concentration of matrine or treatment time(P<00.01),and the antiproliferation effect of matrine on QBC 939 cells showed a doset-ime dependence.The early apoptosis rate,late apoptosis rate and total apoptosis rate increased with the increasing concentration of matrine (P<0.001),and the apoptosis effect of matrine on QBC939 cells showed dose dependence.Rhodamine 123 assay revealed that the proportion of weak fluorescence peak increased with the concentration of matrine increasing(P<0.001).In the groups of concentrations of 0.5,1.0 and 1.5 mg/ml,the expressions of Cyto-c, BAX and activated caspase-3 increased gradually,while the expression of Bcl-2 decreased compared to the controls.Conclusion Matrine has obvious inhibitory effect on the proliferation of QBC939 cells,and can induce the apoptosis of the cells,whose mechanisms may be related to the regulation of the expressions of Bcl-2/BAX proteins in the mitochondrial pathway and the activation of downstream caspase-3.

Objective: To analyze and summarize the hospital medical records of irrational drug use in a tumor hospital during 2013, so as to rationalize drug use.Methods:By a retrospective survey, a total of 1092 hospital medical records were analyzed and the proportion of irrational drug use was calculated. 6 kinds of drugs with frequent use in the hospital were analyzed, including antibiotics, immunomodulators, antineoplastic drugs, Chinese traditional medicine, digestive system drugs and analgesic drug.Results:There were 192 hospital medical records of irrational drug use, with a ratio of 17.58%, among which Chinese patent drugs, antimicrobial agents, and immunomodulators were in a more frequent irrational use.Conclusion:The analysis of hospital medical records shows that irrational drug use still exists at a relatively high level. In order to ensure safe, effective and economic clinical drug use, we should pay attention to the management of the rational drug use, strengthen publicity and training relatively.

Objective To explore the mechanism of matrine inducing apoptosis of HepG 2 cells via mitochondrial apoptotic pathway .Methods MTT assay,PI staining,flow cytometry were used to evaluate the anti-proliferation,cell cycle regulation,and inducing apoptosis effects of matrine on HepG2 cells.Western Blot assay was used to detect the expression levels of anti-apoptotic protein Bid and Bcl-2 in HepG2 cells.Results Matrine could inhibit the proliferation and induce the apoptosis of HepG2 cells in a time-and dose-dependent manner,and the cell cycle of HepG2 was arrested in G0/G1 phase.Apoptosis of HepG2 cells induced by matrine was associated with collapse of mitochondrial membrane potential , which made the Bid and Bcl-2 expression levels down-regulated but the Bax expression level up-regulated.Conclusion Matrine induces the apoptosis of human hepatoma cell line HepG 2 via the mitochondrial apoptotic pathway by regulating the expression levels of Bid ,Bcl-2 and Bax proteins.

AIM This study aimed to investigate the anti-aggregation effects of hexapeptide on human platelets.METHODS Venous blood of healthy volunteer was taken,and was made into anticoagulation blood with sodium citrate,divided into control group,hexapeptide groups(1 × 10-9-1 × 10-5 mol.L-1) and four kinds of positive groups,including clopidogrel group(3 × 10-5mol.L-1),dipyridamole group(2 × 10-6mol.L-1),aspirin group(2.8 × 10-4 mol.L-1) and eptifibatide group(1 × 10-6 mol.L-1).Each group included 6 samples.The inducers were adenosine diphosphate(ADP) + adrenalin,arachidonic acid and thrombin,respectively.The human platelet aggregation rates induced by different inducers were determined with the method of turbidimetry.RESULTS The aggregation inhibition rates of 1 × 10-6 mol.L-1 hexapeptide in human induced by ADP,arachidonic acid and thrombin,were(98.81 ± 2.25) %,(94.58 ± 0.73) % and(33.46 ± 5.56) % respectively,and which were all significant higher than the control group(P < 0.01).Induced by ADP,the anti-platelet aggregation activity of 1 × 10-6 mol.L-1 hexapeptide was significant higher than that of clopidogrel,dipyridamole or aspirin(P < 0.01),but had no different with eptifibatide(P > 0.05).The anti-aggregation IC 50 of hexapeptide of ADP,arachidonic acid and thrombin,were 8.91 × 10-8 mol.L-1,9.33 × 10-8 mol.L-1 and 4.17 × 10-6 mol.L-1,respectively.The anti-aggregation IC 50 of eptifibatide induced by ADP was 1.00 × 10-7 mol.L-1.CONCLUSION Hexapeptide has the effect of anti-platelet aggregation in human in vitro.