目的：检测孕足月胎膜早破孕妇血浆铜、锌、钙、镁、铁的含量和血清及羊水中MMP-9含量变化，探讨微量元素、MMP-9和孕足月胎膜早破的关系。方法选取30例孕足月胎膜早破患者（病例组）和30例正常孕足月患者作对照，用原子吸收法检测其血浆铜、锌、钙、镁、铁五种微量元素的含量，用ELSIA法检测其血清及羊水中MMP-9的含量。结果60例患者中缺钙43例占71．67％；缺铁25例占41．67％；既缺钙又缺铁14例占23．33％。足月胎膜早破组孕妇血浆锌、镁含量高于对照组，差异有统计学意义（ P ＜0．05），足月胎膜早破组孕妇血浆铜、钙、铁较正常对照组差异无统计学意义（ P ＞0．05）；足月胎膜早破组孕妇血清及羊水中MMP-9含量高于对照组，差异有统计学意义（ P ＜0．05）；孕妇血清MMP-9、羊水MMP-9之间有相关性，呈正相关（ P ＜0．05）。结论无论是足月胎膜早破孕妇还是正常孕妇均显示明显缺钙和缺铁，所以孕妇要注意补充钙和铁等微量元素；母体锌、镁、MMP-9含量升高与足月胎膜早破有关。

Objective To investigate the changes of zinc in maternal plasma and MMP-9,collagen Ⅳ levels in serum and matrix metalloproteinases-9 (MMP-9) level in amniotic fluid in patients with premature rupture of membranes at term(TPROM).Methods Thirty cases who were diagnosed as PROM at term during Nov.2012 to Mar.2013 were enrolled as case group,and 30 cases delivered during the same time without PROM were enrolled as control group.Maternal blood and ammiotic fluid were collected from all the cases.The level of zinc in maternal plasma was measured by atomic absorption method and the levels of MMP-9 in serum and amniotic fluid were detected by enzyme linked immunosorbent assay(ELISA),while the level of collagen Ⅳ in serum was measured by up-conversion luminescence method.The relationship among them was analyzed.Results Compared to control group,there were statistically significant difference between TPROM and control groups in terms of the level of zinc,collagen Ⅳ,MMP-9 in serum and MMP-9 in amniotic fluid (zinc:(109.10 ± 16.07) μmol/L vs.(90.54 ± 10.99) μmol/L; t =-5.22,P ＜ 0.001 ; collagen Ⅳ:(56.86 ±41.26) μg/L vs.(88.61 ±44.87) μg/L;t =2.852,P =0.006;MMP-9 in serum:(1 463.25 ±483.6) μg/L vs.(1 196.9 ± 357.43) μg/L,t =-2.426,P =0.018 ; MMP-9 in amniotic fluid:(125.48 ± 67.18) μg/L vs.(72.64 ± 60.74) μg/L,t =-2.873,P =0.006).Zinc level in maternal plasma and collagen Ⅳ in serum had a negative relationship in TPROM (r =-0.261,P =0.044).Zinc level in maternal plasma and MMP-9 level in serum had a positive relationship in TPROM (r =0.274,P =0.034).MMP-9 levels in serum and amniotic fluid had a positive relationship in TPROM (r =0.264,P =0.047).There were no significant relationship between zinc level in maternal plasma,MMP-9 level in amniotic fluid,collagen Ⅳ and MMP-9 levels in serum,collagen Ⅳ in serum and MMP-9 in amniotic fluid (r =0.215,-0.172,-0.172 ; P ＞ 0.05).Conclusion The level of zinc in maternal plasma and increase of MMP-9 in serum and amniotic fluid of women and decrease of the level of collagen Ⅳ in serum are related to the occurrence of TPROM.

卵巢癌是病死率最高的女性恶性生殖系统肿瘤，由于卵巢癌至今缺乏有效的早期诊断方法，所以也称为“沉默杀手”。虽经临床手术治疗及联合化疗后症状会缓解，但多数在1年左右就会复发，并且对多种化疗药物产生耐药性，二线的化学疗法仅对10％～20％的患者有效。因此为了提高疗效并减轻患者的痛苦，临床上急需探索研究疗效好，毒副作用少的抗肿瘤药物。近年发现靶向治疗目标准确、副作用少，是一种很有希望的肿瘤治疗新方法。分子靶向药物是就是利用肿瘤细胞与正常细胞之间分子生物学上的差异（包括基因、酶类、信号转导等不同特性），选择性抑制一些因子或蛋白、酶类的表达，从而抑制肿瘤细胞的生长增殖。靶向治疗在其他肿瘤中已进行了大量研究，对于卵巢癌的治疗研究也取得了很大的进展。

<正>患者,女,28岁,因"停经9+个月、间断右上腹疼痛5+个月,加重5h"于2012年3月3日5:30急诊入院。患者自孕5+月进食后尤其饱食后出现右上腹疼痛,呈持续性绞痛,阵发性加剧,多伴呕吐,能自然缓解,缓解后能平卧。多次就诊于当地各医院均未确诊。5h前再次出现右上腹疼痛,较前加重,不能忍受。入院查体:T 36.7℃,P 84次/min,R 20次/min,BP120/80mmHg;急性痛苦面容,强迫坐位,心肺(-),腹膨隆,肝脾未及,全腹无压痛,未触及宫缩,双下肢水肿(+)。患者不能平卧,未行产科检查。胸膝位肛查:宫颈已展平,位置偏后,宫口开大0.5cm,头/S-1,中骨盆查体不满意。患者10年前曾行膈裂-2