Objective To investigate common chromosomal changes and the LOH frequency of microsatellite loci in primary gastric cancer samples in order to locate the deleted regions in which human gastric cancer related genes might exist.Methods Comparative genomic hybridization (CGH) was used to define global chromosomal aberrations in 43 primary gastric tumors. Based on the results of CGH, analysis of loss of heterozygosity (LOH) was performed in chromosome 19 in which the loss was first discovered in the gastric cancers. The PCR-based approach was used to investigate 22 loci, which are spaced at 1.1-10.9 cM intervals throughout chromosome 19. The amplified PCR fragments were subjected to electrophoresis in PAGE gel and analyzed with GenescanTM and GenotyperTM. Results CGH analysis revealed gains in chromosome 3p(8/43), 8q(8/43), 20 [20 (9/43), 20p(7/43), 20q(4/43)], 12q(16/43), 13q(12/43) and losses in 19 [19 (15/43)], 7 [17 (8/43), 17p (10/43)], 16 (10/43) and 1p (11/43). Among the 43 evaluated samples, the most frequent LOH was detected at locus D19S571 (27.81%). Conclusions The tumorigenesis of gastric cancer includes several chromosomal changes. The aberration of chromosome 19 was the first common change founded in gastric cancer. The region near the D19S571 might harbor potential genes related to the tumorigenesis of gastric cancer.

目的探讨6号染色体长臂上8个肿瘤候选基因与非小细胞肺癌发生发展的关系.方法应用多重PCR对41例非小细胞肺癌中6号染色体长臂上的8个肿瘤候选基因的20个微卫星位点进行扩增.PCR产物应用聚丙烯酰胺凝胶电泳分离,电泳结果用Gene ScanTM,GenotyperTM软件进行分析.结果有7个基因的14个微卫星位点杂合性缺失频率超过20%.分别是CCNC(M74091,34.5%;stSG41342,100%),FYN(stSG29818,22.2%;GDB:187104,38.5%),IGF2R (stSG1519,21.1%;stSG6298,80%),MLLT4 (N26539,25.7%;sts-AA010818, 38.7%),NMBR(stSG6334,50%), PDCD2 (SGC31353, 27.60%;sts-N37094,50%),THBS2(stSG6890,25%;AA131691,29.60%;stSG35838,44.40%).结论这7个肿瘤候选基因可能与非小细胞肺癌发生、发展有关.

目的检测胃癌患者19号染色体微卫星位点的杂合性缺失(less of heterozygosity, LOH),以初步确定19号染色体上与胃癌相关基因连锁最密切的微卫星多态位点.方法应用多重PCR对44例原发性胃癌患者中覆盖19号染色体上的22个微卫星位点(遗传距离在1.1～10.9 cM之间)进行扩增.聚丙烯酰胺凝胶电泳分离PCR产物,Gene ScanTM,GenotypeTM软件进行分析.结果 19个位点(19/22)检测出LOH阳性,总LOH频率为59%(26/44),其中D19S571位点的LOH频率最高(21.43%).结论高频的LOH位点附近,可能存在未知的胃癌相关基因.

Objective To investigate the suppression effect of tumor suppressor gene p53 gene in lung adenocarcinoma cell lines.Methods Wild type p53 gene was transferred with adenovirus vector to a pair of lung adenocarcinoma cell lines with different metastasis potential: Anip973 (high - metastasis potential cell line) and AGZY83-a (low-metastasis potential cell line). The effects of p53 gene infection were evaluated by cell growth curve, western-blotting analysis and TUNEL technique. Results Overexpression of wild-type p53 gene in AGZY83-a, Anip973 inhibited the growth of both lung cancer cells and killed the cells in the end. The suppression effect of wild type p53 gene in Anip973 was higher than AGZY83-a while overexpression of p53 in this pair of cell line. Conclusion Wild-type p53 gene might act as a candidate gene in lung adenocarcinoma gene therapy.