Australian Veterinary JournalVolume 70, Issue 2 p. 72-73 A rapid staining method to differentiate the cytostructure of spermatozoa of farm animals H. RAY, H. RAY Reproductive Biology Research, Indian Institute of Chemical Biology, Jadavpur, Calcutta 700 032, IndiaSearch for more papers by this authorA. BHATTACHARYYA, A. BHATTACHARYYA Reproductive Biology Research, Indian Institute of Chemical Biology, Jadavpur, Calcutta 700 032, IndiaSearch for more papers by this authorA. PAKRASHI, A. PAKRASHI Reproductive Biology Research, Indian Institute of Chemical Biology, Jadavpur, Calcutta 700 032, IndiaSearch for more papers by this author H. RAY, H. RAY Reproductive Biology Research, Indian Institute of Chemical Biology, Jadavpur, Calcutta 700 032, IndiaSearch for more papers by this authorA. BHATTACHARYYA, A. BHATTACHARYYA Reproductive Biology Research, Indian Institute of Chemical Biology, Jadavpur, Calcutta 700 032, IndiaSearch for more papers by this authorA. PAKRASHI, A. PAKRASHI Reproductive Biology Research, Indian Institute of Chemical Biology, Jadavpur, Calcutta 700 032, IndiaSearch for more papers by this author First published: February 1993 https://doi.org/10.1111/j.1751-0813.1993.tb15149.xAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat No abstract is available for this article. References Bean B., Fogle TA and Gates RD (1991) Fertil Steril 55: 214. Bedford JM (1963) J Reprod Fertil 5: 169. Casarett GW (1953) Stain Technol 28: 125. Chacarov EL and Mollova MV (1976) J Reprod Fertil 48: 245. Elder FFB and Hsu TC (1981) Cytogenet Cell Genet 30: 157. Gurr E. (1960) Staining Animal Tissues, Practical and Theoretical, Leonard Hill, England . Pakrashi A. and Ray H. (1978) Lab Anim Sci 28: 348. Volume70, Issue2February 1993Pages 72-73 ReferencesRelatedInformation

A mixture of two partially triterpenoid saponins (Tg), isolated from Acacia auriculiformis was tested for sperm immobilizing activity by using in vitro system. The lowest concentration (ED) required for an obvious immobilization of human sperm by using a modified Sander-Cramer test was found to be 0.35 mg/ml in physiological saline. The ability of the compound as a sperm immobilizing agent was compared with that of Triton X-100 and found to be more potent. Cervical mucus penetration test was also performed and the ED successfully prevented sperm entry in human cervical mucus. Supra-vital staining with eosin-nigrosin indicated death of the treated sperm. Electron microscopic study of Tg-treated sperm showed plasma membrane disintegration and dissolution of acrosomal cap which is presumably the cause for the spermicidal effect of the saponins. No permanent lesion was observed after application of 1.25 mg/ml Tg solution in physiological saline to the eye of rabbits for ten consecutive days.

Oral administration of 3-(4-hydroxy phenyl)-2-propenoic acid, at a dose of 50 mg/kg body weight/day (reported antiprolactin dose in rat) for 56 days, to adult male rat produces complete loss of libido, significant decrease in weight of testis and accessory sex organs, decrease in fructose content of coagulating gland and reduction in acid phosphatase activity in prostate. All these effects can be reversed by exogenous prolactin (500 μg/rat/day) plus testosterone propionate (200 μg/rat/day), but not by prolactin or testosterone alone, when administered along with the test compound for the last 28 days. Inhibitory influence of the compound over the reproductive organs is believed to be attributed primarily to the antiprolactin nature of the compound and secondarily to the significant (P<0.01) fall in plasma testosterone level caused by the compound.