Author(s) Miura, Toshio; Kawaguchi, Akio; Ogawa, Toshiyuki; Ohishi, Tetsuya; Inoue, Kenichiro; Kajiwara, Keiji; Ishikawa, Hiroshi; Yamaguchi, Eiichiro; Yasutaka, Tohru; Shimizu, Teruhisa; Kosei, Miyashita; Hiroyuki, Kusano; Tohru, Nakagoe; Takatoshi, Shimoyama; Ayaba, Hiroyoshi; Tomita, Masao Citation Acta Medica Nagasakiensia. 1992, 37(1-4), p.195-200 Issue Date 1992-12-25 URL http://hdl.handle.net/10069/17588 Right NAOSITE: Nagasaki University's Academic Output SITE

The original publication of the articles cited above included incorrect values.

The original publication of the article cited above included incorrect values in the following part: in Table1 of section (C) Esophageal Surgery.

Title Serum Level of Hyaluronic Acid Does not Correlate with Changes of Hepatic Volume after Portal Vein Embolization Author(s) Nanashima, Atsushi; Yamaguchi, Hiroyuki; Shibasaki, Shinichi; Shindo, Hisakazu; Nakagoe, Tohru; Ayabe, Hiroyoshi Citation Acta medica Nagasakiensia. 2002, 47(1-2), p.43-46 Issue Date 2002-06-18 URL http://hdl.handle.net/10069/16207 Right NAOSITE: Nagasaki University's Academic Output SITE

The original publication of the article cited above included incorrect values in the following part: in Table 1 of section (C) Esophageal Surgery.

The original publication of the article cited above included incorrect values in the following part: in Table1 of section (C) Esophageal Surgery.

Free radical formation on lung allografts was estimated in macrophages of the bronchoalveolar space, and monocytes of the pulmonary artery and pulmonary vein in terms of the different immunosuppressive drugs of cyclosporine and azathioprine. It is elucidated that free radical formation is facilitated by macrophages of the bronchoalveolar space. It is of interest to emphasize that cyclosporine plays a cytoprotective role in prevention of free radical formation.

We report a case of life-threatening reexpansion pulmonary edema following chest tube drainage of spontaneous pneumothorax. Reviewing the literature, pathophysiology, symptoms and therapy of this complication were discussed. Physicians must be aware of the occurrence of reexpansion pulmonary edema following reinflation of

Wound healing at bronchial anastomosis of the lung allograft in canine was compared between allografts prescribed with cyclosporine and/or azathioprine and autografts in terms of recanalization of bronchial artery, breaking strength and histologic findings. 1) Restoration of interrupted bronchial artery completed at the 14 day following transplantation in autografts and allografts with cyclosporine, although it has been retarded until day 21 when azathioparine was given. 2) Satisfactory breaking strength at anastomosis was equally measured in autografts and allografts with cyclosporine. In allografts with azathioprine, weakened breaking strength was noticed on day 14 with significant difference (p<0.05). 3) From the standpoint of histologic finding, cyclosporine is of benefit to eliminate inflammatory response and to promote collagen production which was essential to good wound healing.

Title Numerical Chromosome Aberrations In A Recurrent Malignant Fibrous Histiocytoma of the Retroperitoneum Author(s) Sawai, Terumitsu; Tsuji, Takashi; Yamaguchi, Eiichiro; Nanashima, Atsushi; Jibiki, Masaaki; Yamaguchi, Hiroyuki; Yasutake, Toru; Nakagoe, Tohru; Ayabe, Hiroyoshi; Tagawa, Yutaka Citation Acta medica Nagasakiensia. 1998, 43(3-4), p.88-91 Issue Date 1998-12-16 URL http://hdl.handle.net/10069/16123 Right NAOSITE: Nagasaki University's Academic Output SITE

Calorie restriction (CR) reduces morbidity and mortality in a wide range of organisms, possibly through the stress response machinery. We analyzed the acute phase response of CR rats to lipopolysaccharide (LPS)-induced inflammatory challenge. Six-month-old male F344 rats, fed ad libitum (AL) or a 30% calorie-restricted diet from 6 weeks of age, received an intravenous LPS injection and were then sacrificed between 0 and 8h. CR attenuated liver injury without reduction in the plasma concentrations of proinflammatory cytokines or nitric oxide (NO). Western blotting analysis of liver tissue demonstrated that CR did not affect the degradation of cytoplasmic I-κB and subsequent nuclear translocation of NF-κB, a key transcription factor after inflammatory challenge. We also analyzed the liver gene expression profiles at 0, 1 and 4h with DNA arrays and cluster analysis. Compared with the AL group, CR upregulated the expression of several genes for inflammatory mediators or their related molecules at 0h, but not at 1 or 4h. CR downregulated genes for energy or xenobiotic metabolism and stress response proteins at 0h. At 1h, the relatively downregulated genes by CR were those for proteases and the ubiquitin-proteasome pathway. The present results suggest that CR attenuates liver injury without suppression of the proinflammatory response, and that the protective effect emerges from constitutively, rather than inductively, expressed gene products.

Organ-specific endonuclease might play a role in the age-related increase in apoptosis in laboratory rodent tissues. In nuclear extracts from liver tissues of male F344 rats, the DNase activity gel system identified DNase gamma, Ca(2+)/Mg(2+)-dependent endonuclease. The enzyme activity, which was measured at 3, 6, 16, and 24 months (mo) of age, was significantly increased between 16 and 24mo in control rats fed ad libitum (AL). The expression level of DNase gamma-mRNA, estimated by a semi-quantitative reverse transcription-polymerase chain reaction method, was also increased at 24mo in group AL. The proportion of immunohistochemically DNase gamma-positive cells, most of which were light-microscopically confined to apoptotic cells, was also significantly increased between 16 and 24mo. Dietary restriction, a powerful anti-aging intervention, which was achieved by providing 70% of the mean food intake in group AL from 6 weeks of age, inhibited the age-related increase in the enzyme activity and the proportion of immunostained cells; for the mRNA level, statistical significance was not obtained. The present study suggests that DNase gamma is involved in an age-related increase in the apoptosis of rat liver, and that CR inhibits the increase as it minimized the age-related increase in the fraction of DNA-damaged hepatocytes susceptible to apoptosis.

We investigated immune property of a myoid cell line, established from Fisher rat thymus. Immunization of syngeneic rats with the myoid cells induced anti-rat acetylcholine receptor (AChR). Implantation of them into the thymus failed to induce typical thymic pathology of human myasthenia gravis (MG) or anti-AChR responses. We also demonstrated that the myoid cells were able to present exogenous antigens to T cells and induce antigen-specific T cell proliferation. These results suggest that myoid cells have the potential antigenicity to induce anti-AChR and the functions of antigen-presenting cells, but their expansion in the thymus may not directly cause MG.

A 56-year-old man with a history of alcohol abuse presented with exertional dyspnea. A chest radiography showed a massive right pleural effusion with sanguineous pleural fluid and an amylase level of 97,188 IU/L. Despite conservative treatment with no oral intake, total parenteral nutrition and repeated thoracentesis, the pleural effusion was persistent and intrathoracic infection was suspected. Surgical intervention was proposed and a preoperative endoscopic retrograde cholangiopancreatography revealed disruption of the mid pancreatic duct and a fistulous tract. A middle segment pancreatectomy was performed for removal of the disrupted portion of the main pancreatic duct and reconstruction of the distal pancreas was completed by end-to-side Rouxen-Y pancreatojejunostomy. The patient had a good postoperative course and was discharged on the 29th postoperative day. He has remained well during the 9 months of follow-up.

The adhesion molecule E-cadherin has been shown to influence malignant transformation of tumors, including local and distant metastases. We examined the expression of E-cadherin to determine its relationship to the development of metastasis in metastatic brain tumors. Immunohistochemistry for E-cadherin and Ki-67 was carried out in 76 formalin-fixed, paraffin-embedded archival specimens of metastatic brain tumors and in 14 corresponding available primary tumors from patients who received treatment for metastatic brain tumors. The primary tumors were mainly lung cancers (51.3%), followed by gastrointestinal tumors (28.9%). E-cadherin was expressed in 62 (81.5%) of 76 cases examined. In metastatic adenocarcinomas, a consistent tendency for E-cadherin expression was noted, regardless of the degree of differentiation or the extent of spread of the disease (P=0.4). There was a direct correlation between E-cadherin expression and high MIB-1 index in all metastatic brain tumors (P=0.0007). Pairwise analysis in 14 primary tumors and the corresponding metastatic specimens revealed high E-cadherin and MIB-1 staining in metastatic brain tumors. These results provide a unique association between E-cadherin, systemic metastasis, and proliferation potential in metastatic brain tumors.

胸腔鏡下に手術を施行した縦隔腫瘍41例について, 臨床的に検討した.対象は男性16例, 女性25例, 平均年齢50.4歳, 疾患別には胸腺腫6例, 胸腺嚢腫6例, 神経原性腫瘍12例, 気管支嚢腫7例, 心膜嚢腫2例, 奇形腫2例, その他6例で, 腫瘍の部位は前縦隔17例, 中縦隔8例, 後縦隔13例, 上縦隔3例で腫瘍径は平均4.6cmであった.術式は胸腔鏡のみによる摘出が20例, 小開胸併用16例, 通常開胸への移行例3例, 生検2例であった.ポート数は平均3.1ヵ所, 手術時間は175±78分であった.出血量 (83±160g), 胸腔ドレーン留置期間 (1.1±0.7日), 術後入院期間 (7.7±4.3日) は同時期の胸骨正中切開症例と比較し有意に少なかった.術後合併症は3例 (7.3%) に認めたが, 術死や在院死はなかった.縦隔腫瘍に対する胸腔鏡下手術は低侵襲で安全な手術であり, 小開胸の併用や適切なポートの追加によりどの部位の縦隔腫瘍に対してもアプローチ可能であると考えられた.

The present study was undertaken to evaluate p53 gene mutation as a prognostic factor in patients with colorectal cancer. Nonisotopic RNase cleavage assay (NIRCA), recently used for detecting gene mutations, was employed to detect p53 gene mutations in this study. In 15 samples of colorectal tumors, NIRCA was confirmed to be simple, accurate, and thus useful for clinical use, compared with polymerase chain reaction single-strand conformational polymorphism (PCR-SSCP). In another group of 79 cases of colorectal cancer analyzed for p53 gene mutation by using NIRCA, mutations were detected in 58 of 79 (73.4%) cases. Multivariate Cox proportional-hazards analysis showed that p53 gene mutation was a significant prognostic factor in patients with colorectal cancer. Our results showed that NIRCA is a simple and sensitive method, and thus useful for genetic screening of colorectal cancer. Furthermore, our results showed that p53 gene mutation is an independent predictor of poor prognosis in colorectal cancers.

Title Changes of Branched Chain Amino Acids and Tyrosine Ratio (BTR) after Hepatectomy Author(s) Nanashima, Atsushi; Yamaguchi, Hiroyuki; Shibasaki, Shinichi; Abo, Takafumi; Morino, Shigeyuki; Yoshinaga, Megumi; Sawai, Terumitsu; Tanaka, Kenji; Hidaka, Shigekazu; Tsuji, Takashi; Nakagoe, Tohru; Ayabe, Hiroyoshi Citation Acta medica Nagasakiensia. 2003, 48(1-2), p.29-33 Issue Date 2003-06-25 URL http://hdl.handle.net/10069/16238 Right NAOSITE: Nagasaki University's Academic Output SITE

Background: The aim of this study was to clarify the prognostic value of distal intramural spread of tumor for survival and recurrence in patients with rectal cancer.Methods: Microscopic distal intramural spread was examined in 134 consecutive specimens of resected rectal cancer. Correlations among distal intramural spread, established clinicopathologic factors, and patients' prognoses were examined by univariate and multivariate analyses. American Joint Committee on Cancer classification and stage groupings were used for tumor assessment.Results: Thirty-three patients (24.6%) had distal intramural spread. Multivariate logistical regression analysis revealed that T3/T4 and M1 were independent predictive variables for the presence of distal intramural spread. Patients with distal intramural spread had a shorter disease-specific or disease-free survival time after curative surgery than those without distal intramural spread (P = .0003 and P = .0006, respectively). Most patients with distal intramural spread developed distant recurrence. Cox's regression with multiple covariates showed that distal intramural spread is an independent factor in predicting distant recurrence and worse outcomes after curative surgery in patients with rectal cancer.Conclusions: Distal intramural spread is an independent risk factor for distant metastasis and poor prognosis in patients with rectal cancer.