With a micro-electro-mechanical system (MEMS) mirror, we successfully developed a miniaturized epi-third-harmonic-generation (epi-THG) fiber-microscope with a video frame rate (31 Hz), which was designed for in vivo optical biopsy of human skin. With a large-mode-area (LMA) photonic crystal fiber (PCF) and a regular microscopic objective, the nonlinear distortion of the ultrafast pulses delivery could be much reduced while still achieving a 0.4 microm lateral resolution for epi-THG signals. In vivo real time virtual biopsy of the Asian skin with a video rate (31 Hz) and a sub-micron resolution was obtained. The result indicates that this miniaturized system was compact enough for the least invasive hand-held clinical use.

With a 2D scanning MEMS mirror, we demonstrate a miniaturized epi-third-harmonic-generation microscope with a video rate and a 0.7 mu m transverse resolution. In vivo THG imaging of human skin is demonstrated. (C) 2009 Optical Society of America

With miniaturized tube lenses and a micro-electro-mechanical system (MEMS) mirror, we constructed a miniaturized multiphoton microscope system. Through a two-dimensional asynchronous scanning of the MEMS mirror, 24Hz frame rate can be realized. With a high numerical aperture objective, sub-micron resolution can also be achieved at the same time.

Summary form only given. As a result of the wide use of electronic and optoelectronics devices, the need for an efficient tool to characterize integrated circuits (IC) is growing up. Electric-field-induced-second-harmonic-generation (EFISHG) measurement shows some advantages over an EO sampling technique. We demonstrate a 3D electric-field visualization utilizing the EFISHG effect in nematic liquid crystals. The visualization was easily achieved with a second-harmonic-generation (SHG) confocal microscope with high spatial resolution on the order of or less than 1 /spl mu/m. With the great sectioning power in a confocal microscope, 3D images can be easily obtained combining different height images.

Summary from only given. We present a study using a multi-modal nonlinear microscopy, which reveals nonlinear optical activities in those naturally occurring biophotonic crystalline structures. This technique, based on a femtosecond Cr:forsterite laser at 1230 nm, combines different imaging modalities including second-, third-harmonic generations, and multi-photon fluorescence.