The paper presents the results of an experimental study on the effect of a variable frequency-modulated electric field on the course of a wound process in a burn wound. The evaluation of the effectiveness of the antimicrobial action of wound healing agents against the background of an alternating frequency-modulated electric field has been carried out. The bacteriostatic effect of some wound healing agents used in combination with electrical physical and antiseptic action has been proven.

The results of experimental evaluation of the effectiveness of topical application of the peptide human leukemia differentiation factor-6 in carbopol hydrogel easier to disperse 2020 in thermal burns of skin of III degree are presented. It was found that local application of human leukemia differentiation factor-6 peptide in hydrogel carbopol in concentration from 0,01% to 0,00001% causes acceleration of wound epithelialization processes by 28 days by 71,11% (p0,01) and reduces the incidence of purulent complications by 62,5% (p0,05). Histological examination of biopsy specimens of wounds revealed essential features of morphometry, considering the chosen method of treatment and concentration of peptide of human leukemic factor-6 differentiation in carbopol hydrogel. The proposed method of local treatment of burn skin lesions is very promising for optimizing the flow of reparative regeneration processes. The low frequency of purulent complications in the group of animals treated with a minimum peptide content may be due to the combined action of carbopol hydrogel as determinants of wound healing in a wet environment, and the optimal concentration of the peptide human leukemia differentiation factor-6, providing anti-inflammatory effect in a minimum concentration. Analysis of the histological pattern in the studied groups of animals allows us to conclude about the acceleration of the formation of the extracellular matrix, and epithelialization of the wound surface on the background of an application of the peptide human leukemia differentiation factor-6 minimum concentration. On the 14th day of observation the number of microvessels in granulation tissue among animals treated with human leukemia differentiation factor-6 in carbopol hydrogel easier to disperse 2020 with minimum concentration (0,00001%) was 40,6-43,75% more than in the comparison groups. The use of biologically active peptides is one of the promising methods of wound process optimization. human leukemia differentiation factor-6 peptide can selectively stimulate proliferation by enhancing redox processes in cells.

Results of experimental assessment of efficiency of use of low-temperature air plasma of the arc category of atmospheric pressure at treatment of burns of skin of the III degree in small laboratory animals are given. It is established that use of low-temperature air plasma of the arc-type discharge of atmospheric pressure allows to accelerate terms of final healing of wounds for 49% (p0,05), to reduce development frequency in them purulent inflammation by 45,5% (p0,01). Positive influence of air and plasma processing on dynamics of the area of a hem which after single influence by 35 days is reduced for 67% is revealed (p0,01). The analysis of a histologic picture confirms acceleration of formation of an extracellular matrix and epitelization against the background of impact of an air and plasma stream on wounds. Processing of a wound of low-temperature air plasma of the arc category of atmospheric pressure after an early surgical escharotomy allows to form on a wound surface a so-called nanofilm of a layer the coagulated proteins of wound exudate and cellular fragments which has selective vapor permeability, reduces an rate of wounds pathogenic infection, prevents drying and as a result, allows to reduce the frequency of development of purulent complications and extent of zones of a secondary necrosis. Influence of a stream of low-temperature air plasma of the arc-type discharge of atmospheric pressure allows to optimize significantly processes of reparative regeneration in a zone of a deep burn of skin at a stage of preparation for their surgical treatment.